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Discovery of somatic mutations in the progression of chronic myeloid leukemia by whole-exome sequencing
Authors: Y. Huang, J. Zheng, J.D. Hu, Y.A. Wu, X.Y. Zheng, T.B. Liu and F.L. Chen
We performed whole-exome sequencing in samples representing accelerated phase (AP) and blastic crisis (BC) in a subject with chronic myeloid leukemia (CML). A total of 12.74 Gb clean data were generated, achieving a mean depth coverage of 64.45 and 69.53 for AP and BC samples, respectively, of the target region. A total of 148.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.19.5
- DOI:
- 10.4238/2014.February.19.5
Changes in peripheral blood natural killer T cells in hepatitis B e antigen-positive chronic hepatitis B patients and efficacy prediction after pegylated interferon therapy
Authors: F. Huang, M.H. Lu, H.Y. Gong and Z.P. Xiong
We examined the expression of peripheral blood natural killer T (NKT) cells in hepatitis B e antigen (HBeAg)-positive chronic hepatitis B (CHB) patients and predicted its efficacy after pegylated interferon a-2a (Peg-INFa-2a) therapy. Sixty-three cases of HbeAgpositive CHB inpatients and outpatients, treated in the Third Xiangya Hospital of Central South .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.11.26
- DOI:
- 10.4238/2015.May.11.26
Relationship of polymorphisms in the cholesteryl ester transport protein gene R451Q with coronary heart disease and diabetes in Uyghur and Han Chinese
Authors: C.J. Zhang, H.M. Qiu and J.P. Qiu
To investigate the relationship of polymorphisms in the cholesteryl ester transport protein (CETP) gene with coronary heart disease (CHD) and diabetes in subjects of Uyghur and Han Chinese origin, 266 subjects with CHD including 154 subjects with type 2 diabetes mellitus and 136 healthy subjects (as a control group) were enrol.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.19.6
- DOI:
- 10.4238/2014.February.19.6
Salt stress represses production of extracellular proteases in Bacillus pumilus.
Authors: R.F. Liu, C.L. Huang and H. Feng
Bacillus pumilus is able to secrete subtilisin-like proteases, one of which has been purified and characterized biochemically, demonstrating great potential for use in industrial applications. In the current study, the biosynthesis and transcription of extracellular proteases in B. pumilus (BA06) under salt stress were investigated using various methods, .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.11.27
- DOI:
- 10.4238/2015.May.11.27
Development of expressed sequence tag-based microsatellite markers for the critically endangered Iso�«tes sinensis (Isoetaceae) based on transcriptome analysis
Authors: A.W. Gichira, Z.C. Long, Q.F. Wang, J.M. Chen and K. Liao
Isoëtes sinensis is a critically endangered quillwort. To facilitate studies on the conservation genetics of this species, we developed expressed sequence tag-simple sequence repeat (EST-SSR) markers. A total of 50,063 unigenes were predicted by transcriptome sequencing, 5294 (10.6%) of which significantly matched 3011 Gene Ontology annotations and 2.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038497
- DOI:
- 10.4238/gmr.15038497
Effect of mitochondrial ATP-sensitive potassium channel opening on the translocation of protein kinase C epsilon in adult rat ventricular myocytes
Authors: H. Li, T. Yang, Z. Long and J. Cheng
Twenty healthy male Sprague-Dawley (SD) rats (weight = 180 to 250 g) were supplied by the Animal Center of Daping Hospital of the Third Military Medical University, China. This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.17.3
- DOI:
- http://dx.doi.org/10.4238/2014.June.17.3
Detection of two morphologically cryptic species from the cursor complex (Akodon spp; Rodentia, Cricetidae) through the use of RAPD markers
Authors: G.M. Yazbeck, R.L. Brand�£o, H.M. Cunha and A.P. Paglia
The cursor complex is a group within the Akodon genus of South American rodents, formed by Akodon cursor and A. montensis. Correct distinction between these two species is of great importance since they can harbor different Hantavirus strains. These species are only distinguishable by means of karyotypic or internal anatomic f.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.22.2
- DOI:
- 10.4238/2011.November.22.2
Complete sequence of the mitochondrial genome of the Japanese buff-tip moth, Phalera flavescens (Lepidoptera: Notodontidae)
Authors: Q.-Q. Sun X.-Y. Sun X.-C. Wang Y.-H. Gai J. Hu C.-D. Zhu J.-S. Hao
We sequenced the complete mitochondrial genome of Phalera flavescens. The mitogenome is 15,659 bp in length, including 13 protein-coding genes (atp6, atp8, cox1-3, nad1-6, nad4L, cob), two ribosomal RNAs (rrnS and rrnL), 22 transfer RNAs and an AT-rich region, a putative control region (D-loop). Gene order and orientation were found to be identical to those .. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.10.2
- DOI:
- 10.4238/2012.September.10.2
Semi-quantitative detection of gene expression using bisbenzimide dye
Authors: P. Kittimongkolsuk, T. Tencomnao, R. Santiyanont
An electrochemical biosensor, using a disposable electrochemical printed chip aggregation by the bisbenzimide dye (Hoechst 33258), was used for detecting the expression of b-actin and RAGE genes. Using linear sweep voltammetry, the expression of these two genes in HeLa and HepG2 cell lines was determined based on anodic peak c.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.22.8
- DOI:
- 10.4238/2011.November.22.8
Application of 16S rRNA, cytochrome b and control region sequences for understanding the phylogenetic relationships in Oryx species
Authors: H.A. Khan, I.A. Arif, A.A. Al Homaidan and A.H. Al Farhan
The present study reports the application of mitochondrial markers for the molecular phylogeny of Oryx species, including the Arabian oryx (AO), scimitar-horned oryx (SHO) and plains oryx (PO), using the Addax as an outgroup. Sequences of three molecular markers, 16S rRNA, cytochrome b and a control region, for the above four taxa were aligned and the top.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr514
- DOI:
- 10.4238/vol7-4gmr514