Gene
MEF2A gene mutations and susceptibility to coronary artery disease in the Chinese population
Authors: J. Li1, H.-X. Chen2, J.-G. Yang3, W. Li3, R. Du3 and L. Tian3
We investigated mutations and polymorphisms of the coronary artery disease (CAD)-related myocyte enhancer factor 2A (MEF2A) gene in a Chinese population. Polymerase chain reaction-single-strand conformation polymorphism and DNA sequencing were used to detect exon 11 of the MEF2A gene in 210 Hubei patients with CAD and 190 healthy controls... Read More»
- Genet. Mol. Res. 13(4):
- /2014.October.20.15
- DOI:
- /10.4238/2014.October.20.15
Expression patterns of Doppel in differential ovine PRNP genotypes: quantification using real-time RT-PCR
Authors: C. Wang, C.-L. Zhao, L. Liu, R. Wu and X.-L. Zhang
Doppel is a homologue of cellular prion protein (PrP)-like protein (PrPC). Different tissue samples were collected from the central nervous system plus four regions of lymphoid system, eleven regions of digestive tract and two reproductive organs from four ARR/ARQ and four ARH/ARQ sheep, genotypes of the PrP gene. Total RNA wa.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.3
- DOI:
- 10.4238/2015.October.9.3
Assessment of correlation between pre-miRNA-1757 polymorphism and chicken performance traits
Authors: H. Li, K.R. Jiang, S.H. Wang, X.J. Liu, X.T. Kang, R.R. Jiang,Z.J. Li and G.R. Sun
Single nucleotide polymorphism in microRNAs (miRNA) may influence their target gene selection and regulation efficiency, leading to animal phenotypic variation. The aim of this study was to evaluate the possible effect of single nucleotide polymorphisms in the miRNA-1757 gene precursor region (pre-mir-1757) on economic-related.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.7
- DOI:
- 10.4238/2015.October.9.7
Development of SNP markers and their application for genetic diversity analysis in the oil palm (Elaeis guineensis)
Authors: P.W. Ong, I. Maizura, N.A.P. Abdullah, M.Y. Rafii, L.C.L. Ooi, E.T.L. Low and R. Singh
The genetic evaluation of oil palm germplasm collections is required for insight into the variability among populations. The information obtained is also useful for incorporating new genetic materials into current breeding programs. Single nucleotide polymorphisms (SNPs) have been widely used in many plant genetic studies due .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.9
- DOI:
- 10.4238/2015.October.9.9
Gene expression profiling of somatic and pluripotent cells reveals novel pathways involved in reprogramming
Authors: Y.N. Cai, X.H. Dai, Q.H. Zhang, R.Hu and Z.M. Dai
We investigated gene expression in embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, and fibroblasts. Microarray expression data sets obtained from the Gene Expression Omnibus were analyzed using the Partek software. Human genes from ES cells, iPS cells, and fibroblasts were ranked from low to high according to .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.21
- DOI:
- 10.4238/2015.October.5.21
New microsatellite markers for bananas (Musa spp)
Authors: E.P. Amorim, P.H. Silva, C.F. Ferreira, V.B.O. Amorim, V.J. Santos, A.D. Vilarinhos, C.M.R. Santos, M.T. Souza J�ºnior and R.N.G. Miller
Thirty-four microsatellite markers (SSRs) were identified in EST and BAC clones from Musa acuminata burmannicoides var. Calcutta 4 and validated in 22 Musa genotypes from the Banana Germplasm Bank of Embrapa-CNPMF, which includes wild and improved diploids. The number of alleles per locus ranged from 2 to 14. The markers were .. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.27.8
- DOI:
- 10.4238/2012.April.27.8
Genetic analyses, phenotypic adaptability and stability in sugarcane genotypes for commercial cultivation in Pernambuco
Authors: J.A. Dutra Filho; T.C. Junior and D.E. Sim�µes Neto
In the present study, we assessed the agro-industrial performance of 22 sugarcane genotypes adaptable to edaphoclimatic conditions in production microregions in the State of Pernambuco, Brazil, and we recommended the commercial cultivation of select genotypes. The variables analyzed were as follows: sucrose percentage in cane .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.23
- DOI:
- 10.4238/2015.October.5.23
Effects of BCL2 transfection on the cell cycle and proliferation of human GES-1 cells
Authors: G.Y. Shi, L.L. Zhao, K. Zhang, H.X. Zhou, A.H. Liu, J. Li, G. Li and L.H. Zhu
We investigated the effects of BCL2 transfection on the cell cycle and proliferation of GES-1 cells. A pcDNA3-BCL2 plasmid was used to transfect GES-1 cell line human gastric epithelial cells. Clones were obtained by G418 screening. BCL2-positive cells were identified by fluorescence immunohistochemistry. The pcDNA3-BCL2 vecto.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.15
- DOI:
- 10.4238/2015.October.5.15
DNA methyltransferase 3B -149C/T polymorphism and the risk of laryngeal squamous cell carcinoma: a case-control study
Authors: X.M. Zhang, S. Li and Q.M. Zhang
A variety of molecular epidemiological studies have been conducted to examine the association between the DNMT3B -149C/T polymorphism and cancer susceptibility; however, there has been no study investigating the association between the DNMT3B -149C/T polymorphism and the risk of laryngeal squamous cell carcinoma (LSCC) until now. To determine the role of .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.6
- DOI:
- 10.4238/2015.October.21.6
Construction of a hepatocellular carcinoma cell line that stably expresses stathmin with a Ser25 phosphorylation site mutation
Authors: J. Du, Z.H. Tao, J. Li , Y.K. Liu and L. Gan
We constructed hepatocellular carcinoma (HCC) cells that stably express stathmin with a Ser25 phosphorylation site mutation (stathmin S25A). We used the polymerase chain reaction for site-directed mutagenesis, constructed a stathmin S25A plasmid, and verified the results by restriction enzyme cleavage and sequencing technology.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.24
- DOI:
- 10.4238/2015.October.5.24