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Gene

Plant Genetics   Research Article

Genetic diversity of the Chinese traditional herb Blumea balsamifera (Asteraceae) based on AFLP markers

Authors: Y.X. Pang, W.Q. Wang, Y.B. Zhang, Y. Yuan, J.B. Yu, M. Zhu and Y.Y. Chen

Blumea balsamifera is a commercially important medicinal herb in China and other parts of Asia. It is used to produce borneol. This plant grows in the wild, but resources have diminished greatly in recent years. We examined the genetic diversity of this species to help develop conservation strategies; 35 plants from five provi.. Read More»

Genet. Mol. Res. 13(2):
2014.April.14.1
DOI:
10.4238/2014.April.14.1
Cytogenomics   Research Article

Genetic contribution of CYP2C9, CYP2C19, and APOE variants in acenocoumarol response

Authors: J.A. Nastasi-Catanese1, J.R. Padilla-Guti�©rrez2, Y. Valle2, F. Ortega-Guti�©rrez3, M.P. Gallegos-Arreola4 and L.E. Figuera5,6

Oral anticoagulants of the coumarin type have an inconveniently narrow therapeutic window, making their use difficult. In Mexico, genetic variables that participate in the heterogeneity of the therapeutic response remain poorly investigated. With the focus on warfarin, extensive pharmacogenomic studies have been performed, including those on the CYP450 fa.. Read More»

Genet. Mol. Res. 12(4):
2013.October.10.7
DOI:
10.4238/2013.October.10.7
Human Genetics   Research Article

Complete mitochondrial genome sequence of Marmota himalayana (Rodentia: Sciuridae) and phylogenetic analysis within Rodentia

Authors: Q.J. Chao, Y.D. Li, X.X. Geng, L. Zhang, X. Dai, X. Zhang, J. Li and H.J. Zhang

This is the first report of a complete mitochondrial genome sequence from Himalayan marmot (Marmota himalayana, class Marmota). We determined the M. himalayana mitochondrial (mt) genome sequence by using long-PCR methods and a primer-walking sequencing strategy with genus-specific primers. The complete mt genome of M. himalaya.. Read More»

Genet. Mol. Res. 13(2):
2014.April.14.3
DOI:
10.4238/2014.April.14.3
Medical Genetics   Research Article

Effect of RNAi-mediated silencing of Livin gene on biological properties of colon cancer cell line LoVo

Authors: A.M. Zou, H.F. Wang, W.F. Zhu, F.X. Wang and J.J. Shen

This study aimed to investigate the effect of RNAi-mediated silencing of the Livin gene on biological properties of the colon cancer cell line LoVo. Interference vectors pSilencer4.1-Ll and pSilencer4.1-L2 targeting the Livin gene were constructed and transfected into LoVo cells. The expression of the Livin gene was determined.. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.May.16.8
DOI:
http://dx.doi.org/10.4238/2014.May.16.8
Plant Genetics   Research Article

Isolation and characterization of polymorphic microsatellite markers from Coilia ectenes

Authors: X.J. Rong, Y.J. Xu, Q.Y. Wang, M.J. Liao, X.Z. Liu, C.Y. Pan,Z. Zhang, Y.G. Wang

Coilia ectenes (Jordan and Seale 1905) is an important anadromous species that is an important resource at risk of extinction because of over-fishing, pollution, and coastal construction. To evaluate the genetic diversity of C. ectenes for use in breeding programs, elite microsatellite-enriched libraries were constructed and n.. Read More»

Genet. Mol. Res. 12(4):
2013.November.26.11
DOI:
10.4238/2013.November.26.11
Microbial Genetics   Research Article

Molecular cloning and expression of the porcine S14R gene in Escherichia coli

Authors: Y.J. Guo1*, G.Z. Liu1*, C.M. Wang1, Y.Y. Wang2, H.J. Li2, K. Zhong2, W.F. Lu2, Y.L. Wang2, G.Y. Yang2

We amplified S14R protein gene cDNA of porcine, cloned it into a prokaryotic expression plasmid, and expressed it in Escherichia coli. A pair of primers was designed based on the cDNA sequence of the porcine S14R gene in GenBank. The target gene fragment from porcine liver tissue was amplified by RT-PCR. Confirmed by auto-sequencing, the target gene fragm.. Read More»

Genet. Mol. Res. 12(4):
2013.October.10.6
DOI:
10.4238/2013.October.10.6
Animal Genetics   Research Article

Association of SIRT2 gene polymorphisms with body measurement and growth traits of Qinchuan cattle

Authors: L.S. Gui, W.C. Yang, C.P. Zhao, S.J. Wei, Z.D. Zhao and L.S. Zan

Silent information regulator 2 (SIRT2), a member of the Sirtuin family of class III nicotinamide adenine dinucleotide-dependent protein deacetylases, plays an important role in senescence, metabolism, and apoptosis. This study was conducted to detect potential polymorphisms of the bovine SIRT2 gene and explore their relationships with meat quality and bod.. Read More»

Genet. Mol. Res. 13(4):
Animal Genetics   Research Article

Developmental changes in IGF-I and MyoG gene expression and their association with meat traits in sheep

Authors: W. Sun, R. Su1, D. Li, H.H. Musa, Y. Kong, J.T. Ding, Y.H. Ma, L. Chen, Y.F. Zhang and W.Z. Wu

In the present study, real time-polymerase chain reaction was applied to analyze the expression of IGF-I and MyoG genes in Hu sheep longissimus dorsi at different growth stages and their association with meat traits. Expression of the IGF-I gene in Hu sheep differed significantly between males and females at the two day-old (0.. Read More»

Genet. Mol. Res. 13(2):
2014.April.14.6
DOI:
10.4238/2014.April.14.6
Human Genetics   Research Article

Inbreeding effects in Solanum lycocarpum A. St.-Hil populations, an endangered species of the Brazilian Cerrado

Authors: T.M. Moura, M.V.B.M. Siqueira, G.C.X. Oliveira

The inbreeding effective population size is an estimate of inbreeding and genetic drift in populations. It is an important tool for conservation genetics because it represents the number of individuals that are effectively contributing alleles to the subsequent generations. Several studies have been published in the last decad.. Read More»

Genet. Mol. Res. 12(4):
2013.November.26.10
DOI:
10.4238/2013.November.26.10
Plant Genetics   Research Article

Expression and enzymatic activity of phenylalanine ammonia-lyase and p-coumarate 3-hydroxylase in mango (Mangifera indica �Ataulfo�) during ripening

Authors: H. Palafox-Carlos, C.A. Contreras-Vergara, A. Muhlia-Almaz�¡n, M.A. Islas-Osuna and G.A. Gonz�¡lez-Aguilar

Phenylalanine ammonia lyase (PAL) and p-coumarate 3-hydroxylase (C3H) are key enzymes in the phenylpropanoid pathway. The relative expression of PAL and C3H was evaluated in mango fruit cultivar ‘Ataulfo’ in four ripening stages (RS1, RS2, RS3, and RS4) by quantitative polymerase chain reaction. In addition, enzyme.. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.May.16.10
DOI:
http://dx.doi.org/10.4238/2014.May.16.10