Collagen
Comparison of physical and chemical characteristics of collagen from the skin of cod (Gadus macrocephaius)
Authors: Y. Shu, H. Ren, R. Ao, W.C. Qi and Z.S. Zhang
Acid and pepsin-soluble collagen (ASC and PSC, respectively) were extracted from cod (Gadus macrocephaius) skin, and yields of 37.36 and 55.96% were obtained for ASC and PSC, respectively. The total yield of ASC and PSC was 93.92%, based on the lyophilized dry weight, which is higher than that obtained from other sources. Elec.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16027940
- DOI:
- 10.4238/gmr16027940
Molecular characteristic and expression analysis of collagenolytic serine protease from the Chinese mitten crab Eriocheir sinensis with defense response to Vibrio anguillarum challenge
Authors: Q.Z. Yang, Z.J. Yang, Y. Zhang, X.L. Li and W. Zhang
A novel collagenolytic serine protease (CLSP) was cloned from the hemocytes of the Chinese mitten crab Eriocheir sinensis (Es-CLSP). The full-length cDNA of Es-CLSP contains 990 nucleotides. It encodes a 270-amino acid-long peptide with the mature peptide containing 221 amino acids. It contains the conserved catalytic triad (H.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.1
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.1
Co-transfection of adeno-associated virus-mediated human vascular endothelial growth factor165 and transforming growth factor-�²1 into annulus fibrosus cells of rabbit degenerative intervertebral discs
Authors: Y.M. Xi, Y.F. Dong, Z.J. Wang, Y. Liu, Z.Z. Diao and Y.G. Hu
Intervertebral disc degeneration is a common condition that may lead to low back pain and radiculopathy. Understanding the pathophysiology and cellular and molecular events of degenerative disc disease has resulted in the proposal of a gene therapy approach to halt and reverse disc degeneration. We explored the feasibility of reversing intervertebral disc.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.February.28.11
- DOI:
- 10.4238/2013.February.28.11
A novel frameshift deletion in the COL1A1 gene identified in a Chinese family with osteogenesis imperfecta
Authors: N. Fan, J.B. Jonas, F. He, N.H. Yan, Y. Wang, L. Liu, D.L. Liu, L. Zhao, I.-H. Pang and X.Y. Liu
Osteogenesis imperfecta (OI) is a genetically heterogeneous group of disorders, characterized by abnormal bone fragility, blue sclera, deafness, joint laxity, and soft-tissue dysplasia. The purpose of this study was to elucidate the genetic or molecular basis for OI type IA in a Chinese family. We evaluated the members of a fa.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.30.5
- DOI:
- 10.4238/2015.November.30.5
The role of smoking status and collagen IX polymorphisms in the susceptibility to cervical spondylotic myelopathy
Authors: Z.C. Wang, J.G. Shi, X.S. Chen, G.H. Xu, L.J. Li and L.S. Jia
We investigated a possible association of collagen IX tryptophan (Trp) alleles (Trp2 and Trp3) and smoking with cervical spondylotic myelopathy (CSM) in 172 Chinese patients and 176 age- and gender-matched controls. The smoking status was evaluated by smoking index (SI). The CSM cases had a significantly higher prevalence of T.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.9.2
- DOI:
- 10.4238/2012.May.9.2
Influence of COL1A2 gene variants on the incidence of hypertensive intracerebral hemorrhage in a Chinese population
Authors: D.Z. Tian, W. Wei and Y.J. Dong
ype I collagen (transcribed by COL1A1 and COL1A2 genes) is important for maintaining vessel wall elasticity and is a critical part of the extracellular matrix. We conducted a case-control study to investigate the role of the COL1A2 rs42524 polymorphism in the development of hypertensive intracerebral hemorrhage. Between January 2012 and December 2014, a t.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017369
- DOI:
- 10.4238/gmr.15017369
Molecular response of human cervical and lumbar nucleus pulposus cells from degenerated discs following cytokine treatment
Authors: J.Y. Park Y.S. Yoon H.S. Park S.U. Kuh
We investigated the molecular response of degenerated human cervical and lumbar nucleus pulposus (NP) cells following cytokine treatment. Degenerated cervical and lumbar discs (8 each) were obtained from patients who underwent discectomy for degenerative disc disease; NP cells were isolated and cultured. The mRNA expressions of aggrecan, alkaline phosphat.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.March.15.4
- DOI:
- 10.4238/2013.March.15.4
Inhibitory effects of spironolactone on myocardial fibrosis in spontaneously hypertensive rats
Authors: H. Zhao, D.W. Gu, H.T. Li, Q.F. Ge and G.P. Li
This study evaluated the inhibitory effects of spironolactone, a non-selective aldosterone receptor antagonist, on hypertension-induced myocardial fibrosis. Collagen I and III contents was detected in the myocardial tissue of spontaneously hypertensive rats (SHRs) after spironolactone administration. Twenty male SHRs were assigned to the spironolactone gr.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.August.28.17
- DOI:
- http://dx.doi.org/10.4238/2015.August.28.17
Effects of hydroxycamptothecin on the expression of matrix metalloproteinase-1 (MMP-1), tissue inhibitor of MMP-1, and type I collagen in rats with pulmonary fibrosis
Authors: G.-X. Hu, S.-T. Yao, L.-H. Zeng, Y.-Z. Peng and J. Zheng
The aim of this study was to investigate the effects of hydroxycamptothecin (HCPT) on the expression of matrix metalloproteinase-1 (MMP-1), tissue inhibitor of MMP-1 (TIMP-1), and type I collagen in the lung tissue of rats with pulmonary fibrosis induced by bleomycin A5. We used hematoxylin eosin staining to observe the degree of pulmonary fibrosis in rat.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.4.21
- DOI:
- 10.4238/2015.May.4.21
Effect of co-culturing human primary basic fibroblasts with respiratory syncytial virus-infected 16-HBE cells
Authors: J.-H. Wang, L.-H. Sun, S.-K. Huang and A.-H. Chen
This study investigated the effects induced by co-culturing human primary basic fibroblasts (HPBFs) with 16-human bronchial epithelial cells (16-HBE) infected with respiratory syncytial virus (RSV), in particular the transformation of HPBFs into myofibroblasts and secretion of extracellular matrix proteins. HPBFs were co-cultured with 16-HBE cells infecte.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017339
- DOI:
- 10.4238/gmr.15017339