Molecular cloning and expression of drought-induced protein 3 (DIP3) encoding a class III chitinase in upland rice
Authors: X.L. Guo, L.R. Bai, C.Q. Su, L.R. Shi and D.W. Wang
A drought-induced gene, DIP3, encoding a chitinase III protein was isolated from the roots of upland rice by reverse transcription-polymerase chain reaction (RT-PCR). Sequence analysis demonstrated that the cDNA and deduced protein showed high identity to Oryza sativa class III chitinase. The deduced protein contained a signal peptide sequence in the N-te.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.19.5
- DOI:
- 10.4238/2013.December.19.5
Cloning and characterization of a �²-amyrin synthase gene from the medicinal tree Aralia elata (Araliaceae)
Authors: Y. Wu, H.D. Zou, H. Cheng, C.Y. Zhao, L.F. Sun, S.Z. Su, S.P. Li and Y.P. Yuan
Aralia elata is an important medicinal plant in China; it produces large amounts of oleanane type triterpene saponins. A full-length cDNA encoding β-amyrin synthase (designated as AeAS) was isolated from young leaves of A. elata by reverse transcription-PCR. The full-length cDNA of AeAS was found to have a 2292-bp open reading frame, encoding a prote.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.13.4
- DOI:
- 10.4238/2012.August.13.4
Propofol induces apoptosis and inhibits the proliferation of rat embryonic neural stem cells via gamma-aminobutyric acid type A receptor
Authors: J.W. Wang, W.W. Cheng, T. Xu and Z.Y. Yang
We investigated the effect of propofol on the proliferation and viability of rat embryonic neural stem cells (rENSCs) and the potential mechanisms involved. rENSCs were isolated and cultured in vitro and treated with 1, 10, or 50 μM propofol, while the control group was treated with 0.1 μM dimethyl sulfoxide. Th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.57
- DOI:
- 10.4238/2015.November.18.57
Discovery of a large set of SNP and SSR genetic markers by high-throughput sequencing of pepper (Capsicum annuum)
Authors: M. Nicola�¯, C. Pisani, J.-P. Bouchet, M. Vuylsteke and A. Palloix
Genetic markers based on single nucleotide polymorphisms (SNPs) are in increasing demand for genome mapping and fingerprinting of breeding populations in crop plants. Recent advances in high-throughput sequencing provide the opportunity for whole-genome resequencing and identification of allelic variants by mapping the reads to a reference genome. However.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.13.3
- DOI:
- 10.4238/2012.August.13.3
Using SCC8, SCF27 and VMC7f2 markers in grapevine breeding for seedlessness via marker assisted selection
Authors: M. Akkurt, A. ��ak�±r, M. Shidfar, B.P. ��elikkol and G. S�¶ylemezo��lu
We used molecular markers associated with seedlessness in grapes, namely SCC8, SCF27 and VMC7f2, to improve the efficiency of seedless grapevine breeding via marker assisted selection (MAS). DNA from 372 F1 hybrid progeny from the cross between seeded “Alphonse Lavallée” and seedless “Sultani” was amplified by PCR using thre.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.13.2
- DOI:
- 10.4238/2012.August.13.2
MLH1 and XRCC1 polymorphisms in Mexican patients with colorectal cancer
Authors: R. MuÃ?±iz-Mendoza, M.L. Ayala-Madrigal, M. Partida-PÃ?©rez, J. Peregrina-Sandoval, E. Leal-Ugarte, N. MacÃ?Âas-GÃ?³mez, V. Peralta-Leal, J.P. Meza-Espinoza, J.M. Moreno-Ortiz, R. RamÃ?Ârez-RamÃ?Ârez, S. SuÃ?¡rez-Villanueva and M. GutiÃ?©rrez-Angulo
DNA repair proteins maintain DNA integrity; polymorphisms in genes coding for these proteins can increase susceptibility to colorectal cancer (CRC) development. We analyzed a possible association of MLH1 -93G>A and 655A>G and XRCC1 Arg194Trp and Arg399Gln polymorphisms with CRC in Mexican patients. Genomic DNA samples were obtained from peripheral b.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.June.27.6
- DOI:
- 10.4238/2012.June.27.6
Expression profile of the GA733 gene family in colorectal cancer: correlation with clinicopathological parameters
Authors: I. Mamaloudis, D. Zacharoulis, M. Samara, G. Papadopoulos, S. Samara, G. Koukoulis, C. Chatzitheofilou and P. Kollia
GA733-1/-2/-3 genes have been detected in various types of cancer, although their role has not been fully clarified. GA733-2 and GA733-1 have been correlated with lymph node metastases in laryngeal cancer and liver metastases, respectively. Only a few studies have elucidated the mechanisms regulating GA73.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.42
- DOI:
- 10.4238/2015.November.18.42
Geoffroy�s side-necked turtle [Phrynops geoffroanus (Schweigger, 1812), Testudines: Chelidae] as a model for evolutionary ecotoxicology: relationship between environmental contamination, conditions and genetic variability
Authors: L.P.R. Venancio, D.A.P.C. Zuccari and C.R. Bonini-Domingos
The objective of this study was to evaluate the role of human activity factors, such as environmental contamination and habitat changes, as drivers for changing the physiological, biochemical, and genetic diversity of Geoffroy’s side-necked turtle populations in one of the most impacted watersheds in southeastern Brazil. The impact of chemical and o.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.19.4
- DOI:
- 10.4238/2013.December.19.4
Chromosome number variation and evolution in Neotropical Leguminoseae (Mimosoideae) from northeastern Brazil
Authors: E.C.X.R. Santos, R. Carvalho, E.M. Almeida and L.P. Felix
Most members of the subfamily Mimosoideae have pantropical distributions, variable habits, and a basic chromosome number x = 13. We examined karyotypic evolution of 27 species of this subfamily occurring principally in northeastern Brazil by examining chromosomes stained with Giemsa. All of the species had semi-reticulated interphase nuclei and early cond.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.June.27.1
- DOI:
- 10.4238/2012.June.27.1
Association between HLA-C*04 and American cutaneous leishmaniasis in endemic region of southern Brazil
Authors: R.C. Ribas-Silva, A.D. Ribas, E.C. Ferreira, T.G.V. Silveira and S.D. Borelli
Leishmaniasis is a parasitic infectious disease with global repercussions. American cutaneous leishmaniasis (ACL) is endemic in southern Brazil and its pathogenesis varies according to parasite species, immune response, and host genetics. In terms of immunogenetics, many host genes, including HLA (human leukocyte antigen), cou.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.58
- DOI:
- 10.4238/2015.November.18.58