Differential expression of resistance to powdery mildew at the early stage of development in wheat line N0308
Authors: M.A. Alam, W. Hongpo, Z. Hong and W.Q. Ji
Powdery mildew, caused by Blumeria graminis f. sp tritici (Bgt) is one of the devastating diseases of wheat and causes yield losses in temperate wheat growing regions. A wheat line, N0308 with resistance to powdery mildew was used in this study. A suppression subtractive hybridization cDNA library was constructed from the whea.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.9.15
- DOI:
- http://dx.doi.org/10.4238/2014.June.9.15
Development of primer pairs from diverse chloroplast genomes for use in plant phylogenetic research
Authors: Y.C. Yang, T.L. Kung, C.Y. Hu and S.F. Lin
Variation in the chloroplast DNA sequence is useful for plant phylogenetic studies. However, the number of variable sequences provided by chloroplast DNA for suggested genes or genomic regions in plant phylogenetic analyses is often inadequate. To identify conserved regions that can be used to design primers and amplify variab.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.51
- DOI:
- 10.4238/2015.November.18.51
Comparison between modified DNA extraction protocols and commercial isolation kits in grapevine (Vitis vinifera L.)
Authors: M. Akkurt
Various protocols have been developed and used for DNA extraction in grapevine. However, owing to the long duration of the isolation steps in previously developed protocols, researchers have preferred to use isolation kits for studies in recent years. In our study, the DNA yield and purity obtained using six methods - namely three DNA isolation protocols .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.13.8
- DOI:
- 10.4238/2012.August.13.8
Familial balanced translocation leading to an offspring with phenotypic manifestations of 9p syndrome
Authors: L.S. Abreu, M.S. Brassesco, M.L.C. Moreira, J.M. Pina-Neto
We report two similarly affected cousins (children of monozygotic twin sisters) with phenotypic features consistent with 9p deletion syndrome, including dysmorphic craniofacial features (trigonocephaly, midface hypoplasia, upward-slanting palpebral fissures and long philtrum), intellectual disability and disorders of sex devel.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.9.16
- DOI:
- http://dx.doi.org/10.4238/2014.June.9.16
Characterization of nine microsatellite loci for the tree species Parapiptadenia rigida (Fabaceae-Mimosoideae) and their transferability
Authors: B.F. Costa, L.A. Rodrigues, E.A. Ruas, L.B. Souza, C.F. Ruas, B.G. Vieira, A.R.O. Conson and P.M. Ruas
Parapiptadenia rigida, locally known as angico, is a tropical tree common in the semideciduous Brazilian forest. Its wood is naturally resistant to insect attack and is useful for construction. Extracts from the tree have medicinal properties. We characterized nine microsatellite loci for P. rigida. Thirty-five alleles were detected in a sample of 45 indi.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.13.7
- DOI:
- 10.4238/2012.August.13.7
Vulnerability of atherosclerotic plaques is associated with type I interferon in a murine model of lupus and atherosclerosis
Authors: C.Y. Zhang, B. Qu, P. Ye, J. Li and C.D. Bao
This study aimed to investigate the relationship between type I interferon (IFN-I) and plaque stability in pristane-treated apolipoprotein E-knockout (ApoE-/-) mice. Antinuclear antibody (ANA) and extractable nuclear antigen antibody (ENA) levels were measured by immunofluorescence and enzyme-linked immunospot assay.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.52
- DOI:
- 10.4238/2015.November.18.52
Meta-analysis demonstrates that the NAD(P)H: quinone oxidoreductase 1 (NQO1) gene 609 C>T polymorphism is associated with increased gastric cancer risk in Asians
Authors: Y. Zhang, Z.T. Wang and J. Zhong
The association between the NAD(P)H: quinone oxidoreductase 1 (NQO1) gene C609T polymorphism and gastric cancer has been widely evaluated, yet with conflicting results. Data were available from seven study populations involving 2600 subjects. Overall, comparison of alleles 609T and 609C indicated a significantly increased risk (46%) for gastric cancer (95.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.13.6
- DOI:
- 10.4238/2012.August.13.6
Screening for genes that are differentially-expressed between gastric cancer cells and gastric tumor sphere cells using the gene chip technique
Authors: W.H. Shi, Y.B. Yu, Z.-Y. Li, X.-C. Guo, Z.-L. Wei and Z.-H. Zheng
The purpose of this study was to screen for genes that were differentially expressed between a human gastric carcinoma cell line (HGC-27) and their tumor spheres, using the gene chip technique. The HGC-27 cells and tumor sphere cells were cultured in vitro in a sterile environment. Total RNA was extracted from both sa.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.54
- DOI:
- 10.4238/2015.November.18.54
Microdeletion on 17p11.2 in a Smith-Magenis syndrome patient with mental retardation and congenital heart defect: first report from China
Authors: C. Huang, Y.-F. Yang, H. Zhang, L. Xie, J.-L. Chen, J. Wang, Z.-P. Tan and H. Luo
Smith-Magenis syndrome (SMS) is a rare syndrome with multiple congenital malformations, including development and mental retardation, behavioral problems and a distinct facial appearance. SMS is caused by haploinsufficiency of RAI1 (deletion or mutation of RAI1). We describe an eight-year-old female Chinese patient with multiple malformations, congenital .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.13.5
- DOI:
- 10.4238/2012.August.13.5
Effects of Tripterygium wilfordii glycosides on regulatory T cells and Th17 in an IgA nephropathy rat model
Authors: F. Chen, Y.L. Ma, H. Ding and B.P. Chen
In this study, we examined the effects of Tripterygium wilfordii glycosides (TWGs) on Th17 and regulatory T cells (Tregs) in an immunoglobulin A nephropathy (IgAN) rat model. IgAN model rats were randomly divided into the model group, TWG treatment group, and prednisone group. Normal rats were included as controls. Th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.55
- DOI:
- 10.4238/2015.November.18.55