Association between the Rs3087243 polymorphism and risk for diabetes: a meta-analysis
Authors: J. Liu and H.-X. Zhang
The aim of this study was to evaluate the association between the rs3087243 polymorphism of the cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) gene and the risk of type 1 diabetes mellitus (T1D). A comprehensive meta-analysis of case-control studies was conducted to determine the association between the rs3087243 polymorphism (CT60A/G) and T1D and a.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.6.1
- DOI:
- 10.4238/2013.December.6.1
Association of the tumor necrosis factor-alpha -308G>A polymorphism with breast cancer in Mexican women
Authors: L. GÃ?³mez Flores-Ramos1,2, A. Escoto-De Dios1, A.M. Puebla-PÃ?©rez3, L.E. Figuera-Villanueva4, A. Ramos-Silva1,5, R. RamÃ?Ârez-PatiÃ?±o1,2, J.I. Delgado-Saucedo3, E. Salas-GonzÃ?¡lez6, G.M. ZÃ?ºÃ?±iga-GonzÃ?¡lez7, A. Alonzo-Rojo1,2, I. GutiÃ?©rrez-Hurtado1,2 and M.P. Gallegos-Arreola1
The tumor necrosis factor-alpha (TNF-α) gene plays an important role in cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, insulin resistance, and endothelial function. Polymorphisms of TNF-α have been associated with cancer. We examined the role of the -308G>A polymorphism in this gene by comparing the genotypes.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.17
- DOI:
- 10.4238/2013.November.18.17
Predicting the target genes of microRNA based on microarray data
Authors: B. Cao, T. Ji, B. Zhou, J. Zou, G.Q. Jiao
MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 nucleotides in length, which play important roles in regulating gene expression post-transcriptionally. Several computational methods and algorithms have been developed to predict miRNA targets. In this study, we described a method that can be used to integrate m.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.2.4
- DOI:
- 10.4238/2013.December.2.4
A high-throughput, high-quality plant genomic DNA extraction protocol
Authors: H. Li1,2, J. Li1,2, X.H. Cong2, Y.B. Duan2,3, L. Li2, P.C. Wei2, X.Z. Lu2 and J.B. Yang2
The isolation of high-quality genomic DNA (gDNA) is a crucial technique in plant molecular biology. The quality of gDNA determines the reliability of real-time polymerase chain reaction (PCR) analysis. In this paper, we reported a high-quality gDNA extraction protocol optimized for real-time PCR in a variety of plant species. Performed in a 96-well block,.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.15.1
- DOI:
- 10.4238/2013.October.15.1
Effectiveness of olfactory ensheathing cell transplantation for treatment of spinal cord injury
Authors: Y.J .Rao, W.X. Zhu, Z.Q. Du, C.X. Jia, T.X. Du, Q.A. Zhao, X.Y. Cao and Y.J. Wang
The aim of this study was to determine the effectiveness and safety of transplantation of olfactory ensheathing cells for functional repair of the spinal cord. An olfactory bulb was obtained from a 4- to 5-month-old aborted fetus, and it was digested into single olfactory ensheathing cells and then cultured and purified for 1 .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.30.7
- DOI:
- http://dx.doi.org/10.4238/2014.May.30.7
Efficient oil palm total RNA extraction with a total RNA extraction kit
Authors: S.H. Habib, H.M. Saud and H. Kausar
Oil palm tissues are rich in polyphenols, polysaccharides and secondary metabolites; these can co-precipitate with RNA, causing problems for downstream applications. We compared two different methods (one conventional and a kit-based method - Easy-BlueTM Total RNA Extraction Kit) to isolate total RNA from leaves, roots and shoot apical meristems of tissue.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.3.8
- DOI:
- 10.4238/2014.April.3.8
Comparative genetic diversity of wild and hatchery-produced populations of tongue sole (Cynoglossus semilaevis) using multiplex PCR assays with polymorphic microsatellite markers
Authors: H.S. An, E.-M. Kim, H.W. Kang, H.S. Han, J.W. Lee, J.Y. Park,J.I. Myeong and C.M. An
The tongue sole, Cynoglossus semilaevis (Cynoglossidae), is one of the most economically important fishery resources in Korea. This study presents a preliminary investigation of the future viability of the complete aquaculture of tongue sole in Korea. Specifically, possible differences in genetic variability between wild populations of tongue sole from Ko.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.20
- DOI:
- 10.4238/2013.December.4.20
Identification and authentication of Rosa species through development of species-specific SCAR marker(s)
Authors: K.M.I. Bashir, F.S. Awan, I.A. Khan, A.I. Khan and M. Usman
Roses (Rosa indica) belong to one of the most crucial groups of plants in the floriculture industry. Rosa species have special fragrances of interest to the perfume and pharmaceutical industries. The genetic diversity of plants based on morphological characteristics is difficult to measure under natural conditions due to the i.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.30.8
- DOI:
- http://dx.doi.org/10.4238/2014.May.30.8
Myf5 gene polymorphisms and production performance traits in Songliao white geese
Authors: J.B. Wu, L.P. Cao, M.M. Li, N.N. Du, H.L. Huang and Y. Jin
To explore the relationship between Myf5 gene polymorphisms and production performance traits in Songliao white geese, we used the chicken Myf5 sequence to design primers and amplified part of the exon 1 sequence of the Songliao white goose Myf5 gene. Results of single-strand conformation polymorphism polymerase chain reaction.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.2.3
- DOI:
- 10.4238/2013.December.2.3
A novel splicing site mutation of the GPR143 gene in a Chinese X-linked ocular albinism pedigree
Authors: C.Y. Cai1*, H. Zhu2*, W. Shi1*, L. Su3*, O. Shi4, C.Q. Cai5, C. Ling1 and W.D. Li1
Ocular albinism is an X-linked inherited disease characterized by hypopigmentation of the iris and nystagmus. To identify a new disease-causing mutation of ocular albinism, we collected a Han Chinese pedigree with 7 male congenital nystagmus patients over 3 generations. Slit-lamp photography and optical coherence tomography were performed for the proband... Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.16
- DOI:
- 10.4238/2013.November.18.16