Molecular cloning, sequence characterization, and gene expression profiling of a novel water buffalo (Bubalus bubalis) gene, AGPAT6
Authors: S. Song, J.L. Huo,D.L. Li, Y.Y. Yuan, F. Yuan and Y.W. Miao
Several 1-acylglycerol-3-phosphate-O-acyltransferases (AGPATs) can acylate lysophosphatidic acid to produce phosphatidic acid. Of the eight AGPAT isoforms, AGPAT6 is a crucial enzyme for glycerolipids and triacylglycerol biosynthesis in some mammalian tissues. We amplified and identified the complete coding sequence (CDS) of the water buffalo AGPAT6 gene .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.1.2
- DOI:
- 10.4238/2013.October.1.2
Molecular cloning and site-directed mutagenesis of leucine-based sorting motifs of the porcine invariant chain
Authors: F.Z. Xu, Y. Dai, L.M. Li, X.L. Ding and W.Y. Yu
Invariant chain (Ii) is a transmembrane protein that associates with MHC class II molecules in the endoplasmic reticulum. The cytoplasmic tail of Ii contains two leucine residues able to direct Ii to the endocytic pathway. We obtained the pig Ii gene by RT-PCR. Mutated Ii was prepared via site directed mutagenesis by the PCR Megaprimer method to study the.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.January.4.10
- DOI:
- 10.4238/2013.January.4.10
Genetic diversity of Cosmos species revealed by RAPD and ISSR markers
Authors: A. RodrÃ?Âguez-Bernal, J.L. PiÃ?±a-Escutia, L.M. VÃ?¡zquez-GarcÃ?Âa and A.M. Arzate-FernÃ?¡ndez
The genus Cosmos is native of America and is constituted by 34 species; 28 of them are endemic of Mexico. The cosmos are used as a nematicide, antimalarial, and antioxidative agent. The aim of this study was to estimate the genetic diversity among 7 cosmos species based on random amplified polymorphic DNA (RAPD) and inter-simple sequences repeats (ISSR) m.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.13
- DOI:
- 10.4238/2013.December.4.13
Cellular responses induced in vitro by pestheic acid, a fungal metabolite, in a gastric adenocarcinoma cell line (PG100)
Authors: J.M.C. Sousa, L.A. Matos, D.F.A. Alc�¢ntara, H.F. Ribeiro, L.S. Santos, M.N. Oliveira, L.C. Brito-Junior, A.S. Khayat, A.C. Guimar�£es, L.A. Cunha, R.R. Burbano and M.O. Bahia
There is a constant search for new cancer treatments that are less aggressive and economically affordable. In this context, natural products extracted from plants, fungi, and microorganisms are of great interest. Pestheic acid, or dihidromaldoxin, is a chlorinated diphenylic ether extracted from the phytopathogenic fungus Pestalotiopsis guepinii (Amphisph.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.1.1
- DOI:
- 10.4238/2013.October.1.1
A generic plant RNA isolation method suitable for RNA-Seq and suppression subtractive hybridization
Authors: Y.Q. Zhu1,2, W.J. Wu1,2, H.W. Xiao1,2, H.B. Chen1,2, Y. Zheng1,2, Y.J. Zhang2, H.X.Wang2 and L.Q. Huang1,2
A recently developed revolutionary approach to transcriptomics, RNA-Seq, and suppression subtractive hybridization are powerful tools for gene expression research. However, currently, the difficulty of isolating high-quality RNAs from plant tissues bearing abundant complex polysaccharides, polyphenolics, and secondary metabolites is a serious problem that.. Read More»
- Genet. Mol. Res. 12(4):
Survey and analysis of visual acuity of Kazakhs in different lighting environments
Authors: C.W. Zhang, J.H. Xu, Y.L. Wang, W. Xu and K. Li
The effect of different lighting environments on the vision and refractive error were investigated in 427 Kazakhs (828 eyes) aged from 40 to 60 years old, of which 279 were pastoral (546 eyes) and 148 were urban (282 eyes). Pastoral Kazakhs use natural illumination, whereas city Kazakhs mainly use artificial illumination. Of a.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.3.17
- DOI:
- 10.4238/2014.April.3.17
Influence of the human MIF promoter polymorphism on hepatocellular carcinoma prognosis
Authors: T. Yuan, C. Tang, M. Chen, S. Deng and P. Chen
Hepatocellular carcinoma (HCC) is one of the most common worldwide malignancies. A relative complete diagnosis system for primary carcinoma of liver has already been established, but the surgical prognosis for HCC, which depends mainly on postoperative pathological classification and data of recurrence and metastasis, lacks valid experimental indicators. .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.January.4.3
- DOI:
- 10.4238/2013.January.4.3
Expression profile of insulin-like growth factor system genes in muscle tissues during the postnatal development growth stage in ducks
Authors: C.-L. Song, H.-H. Liu, J. Kou, L. Lv, L. Li, W.-X. Wang and J.-W. Wang
Insulin-like growth factors (IGFs) are regulators that modulate the proliferation and differentiation of muscle tissues. We quantified the messenger RNA (mRNA) expression of IGF-I, IGF-II, and type I and II IGF receptors (IGF-IR and IGF-IIR) in muscle tissues including the breast, leg, and myocardium during an early postnatal development growth stage (pos.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.May.6.3
- DOI:
- 10.4238/2013.May.6.3
Evidence for inbreeding depression in the tree Robinia pseudoacacia L. (Fabaceae)
Authors: C.Q. Yuan, Y.F. Li, L. Wang, K.Q. Zhao, R.Y. Hu, P. Sun, Y.H. Sun, Y. Li, W.X. Gu and Z.Y. Zhou
The magnitude of inbreeding depression within populations is important for the evolution and maintenance of mixed mating systems. However, data are sparse on the magnitude of inbreeding depression in Robinia pseudoacacia. In this study, we compared differences in the mature seed set per fruit, seed mass, germination success, and seedling growth between se.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.12
- DOI:
- 10.4238/2013.December.4.12
Phylogenetic classification and clinical aspects of a new putative Deltapapillomavirus associated with skin lesions in cattle
Authors: T.C. Melo, R.F. Carvalho, J. Mazzucchelli-de-Souza, N. Diniz, S. Vasconcelos, S.L.M.R. Assaf, R.P. Araldi, R.M. Ruiz, I. Kerkis, W. Be�§ak and R.C. Stocc
Bovine papillomaviruses (BPVs) are recognized as causal agents of benign and malignant tumors in cattle. Thirteen types of BPVs have already been described and classified into 3 distinct genera. Divergences in the nucleotide sequence of the L1 gene are used to identify new viral types through the employment of PCR assays with .. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.3.18
- DOI:
- 10.4238/2014.April.3.18