A real-time RT-PCR assay for rapid detection of coxsackievirus A10
Authors: C.Y. Mu, A.Y. Wang, C. Chen, L. Zhao and Z. Li
Enterovirus 71 (EV71) and coxsackievirus A16 (CA16) have been the primary causative agents of hand, foot, and mouth disease (HFMD) outbreaks in mainland China in the past. Hence, the surveillance of HFMD has mostly focused on these viruses. However, in recent years, coxsackievirus A10 (CA10) has also been associated with the increasing sporadic HFMD cases.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.21
- DOI:
- 10.4238/2015.December.21.21
Molecular and chemical characterization of vetiver, Chrysopogon zizanioides (L.) Roberty, germplasm
Authors: R.S. Celestino, M.I. Zucchi, J.B. Pinheiro, J.B. Campos, A.A. Pereira, F.G. Bianchini, R.N. Lima, M.F. Arrigoni-Blank, P.B. Alves and A.F. Blank
Due to the economic interests in vetiver, Chrysopogon zizanioides (L.) Roberty, molecular and chemical studies are essential to generate information for its sustainable exploitation. The aim of this study was to undertake a molecular and chemical characterization of vetiver accessions of the active germplasm bank of the Universidade Federal de Sergipe. Th.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.9
- DOI:
- 10.4238/2015.August.14.9
Receptor for advanced glycation end-products (RAGE) is overexpressed in human osteosarcoma and promotes the proliferation of osteosarcoma U-2OS cells in vitro
Authors: Q. Zhang, Y. Jin, C.F. Zhao, W.J. Wang and G.Y. Liu
Osteosarcoma (OS) is an aggressive cancer of the long bones, and usually affects children and young adults. The receptor for advanced glycation end-products (RAGE) has recently been recognized as an oncogenic receptor that binds to different ligands, and promotes the progression of various cancers. However, little is known abo.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027817
- DOI:
- 10.4238/gmr.15027817
Multi-modulus algorithm based on global artificial fish swarm intelligent optimization of DNA encoding sequences
Authors: Y.C. Guo, H. Wang, H.P. Wu and M.Q. Zhang
Aimed to address the defects of the large mean square error (MSE), and the slow convergence speed in equalizing the multi-modulus signals of the constant modulus algorithm (CMA), a multi-modulus algorithm (MMA) based on global artificial fish swarm (GAFS) intelligent optimization of DNA encoding sequences (GAFS-DNA-MMA) was proposed. To improve the conver.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.23
- DOI:
- 10.4238/2015.December.21.23
N-ethylmaleimide-sensitive factor siRNA improves cardiac function following myocardial infarction in rats
Authors: Y. Zhou , Y. Liu, S.X. Yang and Z. Wang
This study examined the effects of N-ethylmaleimide-sensitive factor (NSF) small interfering RNA (siRNA) on cardiac function following myocardial infarction (MI) in rats. Thirty-six adult Sprague Dawley rats were randomly divided into three equivalent groups. An acute MI model was established by ligating the anterior descending branch of the left coronary.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.11
- DOI:
- 10.4238/2015.August.14.11
Overexpression of an endo-1,4-�²-glucanase V gene (EGV) from Trichoderma reesei leads to the accumulation of cellulase activity in transgenic rice
Authors: X.Y. Li, F. Liu, Y.F. Hu, M. Xia, B.J. Cheng, S.W. Zhu and Q. Ma
The ectopic expression of cellulase in biomass can reduce the cost of biofuel conversion. This trait modification technique is highly beneficial for biofuel production. In this study, we isolated an endo-1,4-beta-glucanase gene (EGV) from Trichoderma reesei and inserted this gene downstream of a fragment encoding the signal peptide Apo-SP in a modified pC.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.24
- DOI:
- 10.4238/2015.December.21.24
Aldehyde dehydrogenase 2 protects human umbilical vein endothelial cells against oxidative damage and increases endothelial nitric oxide production to reverse nitroglycerin tolerance
Authors: X.Y. Hu, Q. Fang, D. Ma, L. Jiang, Y. Yang, J. Sun, C. Yang and J.S. Wang
Medical nitroglycerin (glyceryl trinitrate, GTN) use is limited principally by tolerance typified by a decrease in nitric oxide (NO) produced by biotransformation. Such tolerance may lead to endothelial dysfunction by inducing oxidative stress. In vivo studies have demonstrated that aldehyde dehydrogenase 2 (ALDH2) plays impor.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027822
- DOI:
- 10.4238/gmr.15027822
Effects of washed platelets vs platelet-rich plasma on the proliferation and mineralization of rat dental pulp cells
Authors: L. Zhang, Y.H. Xie and B.R. Lin
We examined the effects of washed platelets (WPLTs) and platelet-rich plasma (PRP) on the proliferation and mineralization of rat dental pulp cells. Rat dental pulp cells were separated, cultured, and identified. Medium containing 1, 10, 100, or 500 mL/L PRP or WPLTs was added to 4th generation cells. The MTS method was used to determine cell proliferatio.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.12
- DOI:
- 10.4238/2015.August.14.12
Association between PDCD1, CTLA4, and MECP2 gene polymorphisms and systemic lupus erythematosus in the Chinese Northern Han
Authors: H.R. Dong, H.S. Li, S.C. Wang, Q.M. Balin and P.Y. Chang
Systemic lupus erythematosus (SLE) is an autoimmune disease that results in chronic inflammation of different organ systems. Several susceptibility loci for SLE have been suggested in different populations, but the nature of the susceptibility genes has yet to be determined. The programmed cell death 1 gene (PDCD1), the cytotoxic T-lymphocyte-associated p.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.29
- DOI:
- 10.4238/2015.December.21.29
Estimation of genetic distance based on RAPDs between 11 cotton accessions varying in heat tolerance
Authors: A.I. Khan, I.A. Khan, F.S. Awan, H.A. Sadaqat and S. Bahadur
The genetic distance of 11 cotton genotypes varying in heat tolerance was studied using RAPD markers. Fifty-three random decamer primers were used for the estimation of genetic distance. Among the 53 RAPD primers, which were custom synthesized by GeneLink Inc., UK, 32 were polymorphic and 21 were monomorphic. The 32 polymorphi.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr835
- DOI:
- 10.4238/vol10-1gmr835