Mechanism of rat osteosarcoma cell apoptosis induced by a combination of low-intensity ultrasound and 5-aminolevulinic acid in vitro
Authors: Y.N. Li, Q. Zhou, B. Yang, Z. Hu, J.H. Wang, Q.S. Li and W.W. Cao
We investigated the killing effect of low-intensity ultrasound combined with 5-aminolevulinic acid (5-ALA) on the rat osteosarcoma cell line UMR-106. Logarithmic-phase UMR-106 cells were divided into a control group, ultrasound group and 5-ALA group. The cell apoptotic rate, production of reactive oxygen species, and the change in mitochondrial membrane p.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.23
- DOI:
- 10.4238/2015.August.14.23
Incidence of fibroblast growth factor receptor 3 gene (FGFR3) A248C, S249C, G372C, and T375C mutations in bladder cancer
Authors: Y. Dodurga, C. Tataroglu, Z. Kesen and N.L. Satiroglu-Tufan
Bladder cancer is the most frequent cancer of the urinary system. Fibroblast growth factor receptors (FGFR) belong to the tyrosine kinase family and have important roles in cell differentiation and proliferation and embryogenesis. FGFR3 is located on chromosome 4p16.3, and missense mutations of FGFR3 are associated with autoso.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr923
- DOI:
- 10.4238/vol10-1gmr923
MicroRNA profiling in cutaneous wounds of diabetic rats
Authors: Y.-F. Liu, M. Ding, D.-W. Liu, Y. Liu, Y.-G. Mao and Y. Peng
Despite years of effort, current therapies for diabetic wounds are still not fully efficacious. Emerging evidence has suggested that microRNAs (miRNAs) play key roles in multiple physiological and pathological processes in eukaryotes, and could potentially be powerful therapeutic tools. This study investigated the differential expression profiling of miRN.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.24
- DOI:
- 10.4238/2015.August.14.24
cDNA, genomic sequence cloning and overexpression of the ribosomal protein S13 gene in the giant panda (Ailuropoda melanoleuca)
Authors: Y. Song, Y.-L. Hou, W.-R. Hou, G.-F. Wu and T. Zhang
The cDNA and the genomic sequence of ribosomal protein S13 (RPS13) of the giant panda (Ailuropoda melanoleuca) was cloned using reverse transcription-polymerase chain reaction (RT-PCR) and touchdown-PCR, respectively. These two sequences were sequenced and analyzed, and the cDNA of the RPS13 gene was overexpressed in Escherich.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1000
- DOI:
- 10.4238/vol10-1gmr1000
Adaptability and stability of soybean genotypes in off-season cultivation
Authors: R.O. Batista, R.L. Hamawaki, L.B. Sousa, A.P.O. Nogueira and O.T. Hamawaki
The oil and protein contents of soybean grains are important quantitative traits for use in breeding. However, few breeding programs perform selection based on these traits in different environments. This study assessed the adaptability and stability of 14 elite early soybean breeding lines in off-season cultivation with respect to yield, and oil and prot.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.26
- DOI:
- 10.4238/2015.August.14.26
Characteristics of mature wheat embryos with different resistance to scab cultured in vitro with Fusarium graminearum crude toxin
Authors: S. Wang, M. Wang and C.Y. Zhang
Five wheat varieties with different resistance to scab were used to study the effect of Fusarium graminearum crude toxin on culture characteristics of mature wheat embryos in vitro. The results showed that the rate of induction and differentiation of mature embryo callus differed significantly between varieties, toxin concentration, and concentration x va.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.3
- DOI:
- 10.4238/2015.December.21.3
Estimating the genetic parameters for liver fat traits in broiler lines divergently selected for abdominal fat
Authors: M.J. Liang, Z.P. Wang, L. Xu, L. Leng1, S.Z. Wang, P. Luan, Z.P. Cao, Y.M. Li and H. Li
Intensive selection of broilers for improved growth rate is known to exert a negative effect on broiler health, such as an increase in body fat (and its related diseases). Excessive fat deposition in the liver can cause fatty liver hemorrhagic syndrome (FLHS); in addition, traits associated with liver fat have also been associated with FLHS. This study ex.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.27
- DOI:
- 10.4238/2015.August.14.27
Genetic diversity and differentiation in Dalbergia sissoo (Fabaceae) as revealed by RAPD
Authors: B.-Y. Wang, L. Shi, Z.-Y. Ruan and J. Deng
Dalbergia sissoo, a wind-dispersed tropical tree, is one of the most preferred timber tree species of South Asia. Genetic diversity and differentiation among natural populations of D. sissoo were examined for the first time. We found a relatively high level of genetic diversity in D. sissoo, both at the species level (percenta.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr995
- DOI:
- 10.4238/vol10-1gmr995
Effect of TIMP1 transfection on PTEN expression in human kidney proximal tubular cells
Authors: J.X. Chen, G.Y. Cai, X.M. Chen, H. Liu, X. Chen, Y.M. Peng, F.Y. Liu, Z. Li and S.Z. Shi
To explore the role of metalloproteinase-1 (TIMP-1) tissue inhibitor in the mechanisms of kidney aging, we observed the effects of sense and antisense transfection of TIMP-1 and of metalloproteinase (MMP) inhibitors on phosphatase and tensin homolog (PTEN), vascular endothelial growth factor (VEGF), and Flk-1 expression in TIMP-1 transgenic human proximal.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.6
- DOI:
- 10.4238/2015.December.21.6
Toll-like receptor 4 promotes fibrosis in bleomycin-induced lung injury in mice
Authors: X.X. Li, D.Y. Jiang, X.X. Huang, S.L. Guo, W. Yuan and H.P. Dai
The specific role of Toll-like receptor 4 (TLR4) in bleomycin-induced lung fibrosis of mice, a model of human idiopathic pulmonary fibrosis, has not been characterized. We injected bleomycin intratracheally into TLR4 knockout (TLR4-/-) and wild-type (WT) mice. Twenty-one days after injection, mice were sacrificed and their lungs were harvested for patholo.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.8
- DOI:
- 10.4238/2015.December.21.8