Limitation of high-resolution melting curve analysis for genotyping simple sequence repeats in sheep
Authors: M. Yang, Y.J. Yue, T.T. Guo, J.L. Han, J.B. Liu, J. Guo, X.P. Sun, R.L. Feng, Y.Y. Wu, C.F. Wang, L.P. Wang and B.H. Yang
Variation in microsatellite or simple sequence repeat (SSR) loci has, until recently, relied heavily on the use of gel-based methods that can be both time consuming and difficult to genotype. Non gel-based systems are therefore important to increase simplicity and improve turn-around time without compromising assay sensitivity.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.8.7
- DOI:
- 10.4238/2014.April.8.7
Comparative proteomic analysis between early developmental stages of the Coffea arabica fruits
Authors: G.B. Bandil1, R.M. Etto2, C.W. Galv�£o2, H.J.O. Ramos3, E.M. Souza4, F.O. Pedrosa4, D.F.S. Chaves4, L.F. Huergo4 and R.A. Ayub1
Coffee is one of the most valuable agricultural commodities. There is much agronomic research on coffee, but molecular knowledge of its fruit development and ripening is limited... Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.29.4
- DOI:
- 10.4238/2013.October.29.4
Preventive effect of tert-butylhydroquinone on scrotal heat-induced damage in mouse testes
Authors: Y.S. Li1, Y.G. Piao1, K. Nagaoka2,3, G. Watanabe2,3, K. Taya2,3 and C.M. Li1
To investigate the effect of tert-butylhydroquinone (tBHQ) on scrotal heat-induced damage in mice testes, 8-week-old mice were divided into 6 groups and administered with or without tBHQ through diet (10 mg/g), intraperitoneal injection (100 mg/kg body weight), or intratestis injection (12.5 mg/kg body weight), respectively. After single scrotal heat expo.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.11.5
- DOI:
- 10.4238/2013.November.11.5
Antibody-based detection of alkaline phosphatase in lepidopteran insects (Lepidoptera: Noctuidae)
Authors: R.A. Khan1, M. Rashid1, D. Wang2 and Y.L. Zhang1
Alkaline phosphatase (ALP) of Helicoverpa armigera Hub. (Lepidoptera; Noctuidae) (GenBank accession No. EU729322) was cloned and expressed. The target gene H.a-ALP, having an open reading frame of 1608 bp, was reverse-transcribed from cDNA by the polymerase chain reaction. The open reading frame of the target gene was cloned into the pET-32a expression ve.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.10.3
- DOI:
- 10.4238/2013.October.10.3
Global comparison of gene expression between subcutaneous and intramuscular adipose tissue of mature Erhualian pig
Authors: W.X. Sun1, H.H. Wang1,2, B.C. Jiang1, Y.Y. Zhao1, Z.R. Xie1, K. Xiong1 and J. Chen1
Adipose tissue plays an important role in energy metabolism and related diseases. The content of intramuscular fat significantly influences the pork quality. In this study, the whole gene expression of dorsal subcutaneous (s.c.) adipose tissue and intramuscular (i.m.) adipose tissue isolated from longissimus dorsi muscle tissue were compared using Affymet.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.29.3
- DOI:
- 10.4238/2013.October.29.3
Karyotype variation in cultivars and spontaneous cocoa mutants (Theobroma cacao L.)
Authors: G.S.F. Figueiredo, C.A.F. Melo, M.M. Souza, I.S. Ara�ºjo, H.A. Zaidan, J.L. Pires and D. Ahnert
Four mutant cocoa accessions with morphological changes and a cultivar sample were karyomorphologically characterized. Slides were prepared by enzymatic digestion of the root meristem and squashed in 45% acetic acid, followed by 2% Giemsa staining. The chromosome number of 2n = 20 was seen in all accessions. The karyotype formula for Cacau Comum and Cacau.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.5
- DOI:
- 10.4238/2013.October.18.5
Overexpression of an alternative oxidase gene, OsAOX1a, improves cold tolerance in Oryza sativa L.
Authors: C.R. Li1,2*, D.D. Liang1,2*, R.F. Xu1,2*, H. Li2,3, Y.P. Zhang2, R.Y. Qin2, L. Li1,2, P.C. Wei2,3 and J.B. Yang2
Low temperature is a major environmental stress in rice cultivating and production. The alternative oxidase 1 (AOX1) gene is potentially important for genetic engineering to increase cold adaptation. However, previous studies related to this effect have mostly focused on the dicot plants Arabidopsis and tobacco, whereas functional research on rice is limi.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.11.4
- DOI:
- 10.4238/2013.November.11.4
An efficient algorithm for finding attractors in synchronous Boolean networks with biochemical applications
Authors: D. Zheng, G. Yang, X. Li, Z. Wang and W.N.N. Hung
Self-organized systems, genetic regulatory systems and other living systems can be modeled as synchronous Boolean networks with stable states, which are also called state-cycle attractors (SCAs). This paper summarizes three classes of SCAs and presents a new efficient binary decision diagram based algorithm to find all SCAs of synchronous Boolean networks.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.4
- DOI:
- 10.4238/2013.October.18.4
Genome-wide analysis of the homeodomain-leucine zipper (HD-ZIP) gene family in peach (Prunus persica)
Authors: C.H. Zhang, R.J. Ma, Z.J. Shen, X. Sun, N.K. Korir and M.L. Yu
In this study, 33 homeodomain-leucine zipper (HD-ZIP) genes were identified in peach using the HD-ZIP amino acid sequences of Arabidopsis thaliana as a probe. Based on the phylogenetic analysis and the individual gene or protein characteristics, the HD-ZIP gene family in peach can be classified into 4 subfamilies, HD-ZIP I, II.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.8.8
- DOI:
- 10.4238/2014.April.8.8
Association of NPP1 polymorphism with postoperative progression of ossification of the posterior longitudinal ligament in Chinese patients
Authors: Z. He, H. Zhu, L. Ding, H. Xiao, D. Chen and F. Xue
Ossification of the posterior longitudinal ligament (OPLL) is a condition of the spine that can cause paralysis by compressing the spinal cord. The aim of this study was to evaluate the possible role of nucleotide pyrophosphatase phosphodiesterase 1 gene (NPP1) polymorphism in the etiology and pathology of the OPLL in Chinese patients. DNA from patients w.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.3
- DOI:
- 10.4238/2013.October.18.3