Dynamic changes of virus load in supernatant of primary CEK cell culture infected with different generations of avian infectious bronchitis virus strains Sczy3 as revealed by real-time reverse transcription-polymerase chain reaction
Authors: Z.J. Wei, F.Y. Wang, M.P. Guo, Z.Z. Duan, N.L. Zou, P. Liu, Q.G. Yan, X.T. Wen, S.J. Cao and Y. Huang
Infectious bronchitis virus (IBV) can multiply effectively in chick embryo kidney (CEK) cells after adapting to the chick embryo. To investigate the dynamic changes in IBV load in the supernatant of primary CEK cells, we developed an SYBR Green I-based real-time polymerase chain reaction assay to quantify nucleic copy numbers of the IBV-Sczy3 strain. The .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.9
- DOI:
- 10.4238/2015.June.11.9
Whole-exome analysis of a child with polycystic kidney disease and ventriculomegaly
Authors: M.M. Nabhan,H. Abdelaziz,Y. Xu,R. El Sayed,M. Santibanez-Koref,N.A. Soliman and J.A. Sayer
Autosomal recessive polycystic kidney disease (ARPKD) is an inherited ciliopathy leading to progressive kidney and liver disease. Biallelic mutations in the PKHD1 gene underlie this condition. We describe a child with bilaterally enlarged cystic kidneys, portal hypertension, and cerebral ventriculomegaly. Molecular genetic investigations using whole-exome.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.17.11
- DOI:
- http://dx.doi.org/10.4238/2015.April.17.11
Positive association between PPARD rs2016520 polymorphism and coronary heart disease in a Han Chinese population
Authors: H.D. Ye, Y.R. Li, Q.X. Hong, A.N. Zhou, Q.L. Zhao, L.M. Xu, M.Q. Xu, X.T. Xu, L.L. Tang, D.J. Dai, D.J. Jiang, Y. Huang,D.W. Wang and S.W. Duan
PPARD encodes peroxisome proliferator-activated receptor delta, which has been shown to play an important role in controlling lipid metabolism and atherosclerosis. In this case-control study, we explored the relationship between PPARD rs2016520 polymorphism and coronary heart disease (CHD) in a Han Chinese population. A total of 657 CHD cases and 640 cont.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.10
- DOI:
- 10.4238/2015.June.11.10
Activity of matrix metalloproteinases 2 and 9 in cultured rabbit corneal epithelium cells stimulated by tumor necrosis factor alpha
Authors: Z.-Q. Wu, Z.-L. Zhang, S.-W. Nie, J. Yuan and Y.-N. Yang
We studied the activity of matrix metalloproteinases (MMP) 2 and 9 generated by cultured rabbit corneal epithelium cells that had been stimulated with tumor necrosis factor alpha (TNF-a), to investigate the possible regulative mechanisms of MMP-2/9 and their potential effect on corneal inflammatory diseases. The rabbit corneal epithelium cells were cultur.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.11
- DOI:
- 10.4238/2015.June.11.11
Polymorphisms in the nuclear factor kappa B gene association with recurrent embryo implantation failure
Authors: L. Luo, D.H. Li, X.P. Li, S.C. Zhang, C.F. Yan, J.F. Wu, Y.H. Qi and J. Zhao
Despite more than a century of intensive study, the mechanisms of successful pregnancy remain unclear. Recent research suggests that NF-κB (nuclear factor kappa B) plays an important role in embryo implantation. In the current study, we aimed to identify SNPs that contribute to genetic susceptibility for recurrent implan.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027759
- DOI:
- 10.4238/gmr.15027759
Cloning and analysis of the soybean MEKK gene
Authors: A.-H. Sha,H.-P. Ba,Z.-H. Shan,H.-F. Chen,S.-L. Chen,D.-Z. Qiu,X.-A. Zhou and Y.-H. Chen
In this paper, homologous cloning methods were used to clone the soybean GmMEKK gene, which possesses a high degree of similarity to Arabidopsis thaliana AtMEKK1. AtMEKK1 is formed by 595 amino acids, and its secondary structure is formed by 38 irregular curls, 24 α helix, 14 β, with S-TKc domain, transmembrane domain and does not have membrane.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.17.12
- DOI:
- http://dx.doi.org/10.4238/2015.April.17.12
A simplified genomic DNA extraction protocol for pre-germination genotyping in rice
Authors: Y.B. Duan, F.L. Zhao, H.D. Chen, H. Li, D.H. Ni, P.C. Wei, W. Sheng, J.T. Teng, A.M. Zhang and J.P. Xue
Genotyping is a critical step for molecular markerassisted selection in rice. Rice genomic DNA samples for genotyping are typically isolated from living tissues such as seedlings. This requires the germination of all candidate seeds and extraction of DNA from the seedlings. Currently, an ideal individual is selected from a very large number of plants, whi.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.12
- DOI:
- 10.4238/2015.June.11.12
A replicating plasmid-based vector for GFP expression in Mycoplasma hyopneumoniae
Authors: H.Z.A. Ishag, M.J. Liu, R.S. Yang, Q.Y. Xiong, Z.X. Feng and G.Q. Shao
Mycoplasma hyopneumoniae (M. hyopneumoniae) causes porcine enzootic pneumonia (PEP) that significantly affects the pig industry worldwide. Despite the availability of the whole genome sequence, studies on the pathogenesis of this organism have been limited due to the lack of a genetic manipulation system. Therefore, the aim of.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027832
- DOI:
- 10.4238/gmr.15027832
FlgN plays important roles in the adhesion of Aeromonas hydrophila to host mucus
Authors: X. Jiang, Y.X. Qin, G.F. Lin, L. Huang, B. Huang, W.S. Huangand Q.P. Yan
Adhesion to the host mucus is a crucial step in the early infection stage of pathogenic bacteria. To investigate the mechanisms of the adhesion of Aeromonas hydrophila to its host mucus, a mutant library was constructed using the mini-Tn10 transposon mutagenesis system. Of 276 individual colonies, the mutant strain with the most attenuated adhesion abilit.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.13
- DOI:
- 10.4238/2015.June.11.13
Clinical value of concurrent radiochemotherapy in cervical cancer and comparison of ultrasonography findings before and after radiochemotherapy
Authors: W.M. Yan,X.Z. Li,Z.L. Yu,J. Zhang and X.G. Sun
Herein, we investigated the clinical value of concurrent radiochemotherapy for patients with advanced cervical cancer and its effects on adverse clinical symptoms. Forty patients with cervical cancer were recruited from January 2011 to January 2014 for this study. Participants were randomly allocated into a test or control group, with 20 patients in each .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.17.13
- DOI:
- http://dx.doi.org/10.4238/2015.April.17.13