Cloning, characterization, and expression of the macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (Penaeidae)
Authors: D.G. Zeng, A.Y. Lei, X.H. Chen
The macrophage migration inhibitory factor (MIF) is an important proinflammatory cytokine that mediates both innate and adaptive immune responses. In this study, we identified a homolog of MIF in the Pacific white shrimp Litopenaeus vannamei. The MIF cDNA contained a 363-bp open reading frame encoding a 120-amino acid protein .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.22.15
- DOI:
- 10.4238/2013.November.22.15
Genetic diversity in natural populations of Theobroma subincanum Mart. in the Brazilian Amazon
Authors: L.H. Rivas1, L.D. Giustina1, L.N. Luz2, I.V. Karsburg1, T.N.S. Pereira2 and A.A.B. Rossi1
The genus Theobroma, recently reclassified in the family Malvaceae, comprises some species with high economic potential, including the cupuí, Theobroma subincanum Mart., which has not yet been domesticated, and whose genetics and population structure are mostly unknown. This study aimed to assess the population structure and genetic diversity in na.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.24.12
- DOI:
- 10.4238/2013.October.24.12
Association of novel single-nucleotide polymorphisms of the vascular endothelial growth factor-A gene with high-altitude adaptation in yak (Bos grunniens)
Authors: X.Y. Wu1,2, C.N. Liang1,2, X.Z. Ding1,2, X. Guo1,2, P.J. Bao1,2, M. Chu1,2, W.B. Liu1,2 and P. Yan1,2
Vascular endothelial growth factor-A gene (VEGF-A) is a key regulator of angiogenesis and an endothelial cell mitogen that plays an important role in high-altitude adaptation. In this study, we detected 2 novel single-nucleotide polymorphisms (SNPs) of VEGF-A by screening for genetic variation in 700 individuals of 3 domestic Chinese yak breeds - namely G.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.1
- DOI:
- 10.4238/2013.November.18.1
Tumor-associated fibroblast-conditioned medium promotes tumor cell proliferation and angiogenesis
Authors: L.N. Xu, B.N. Xu, J. Cai, J.B. Yang, N. Lin
This study aimed to explore how tumor-associated fibroblasts (TAFs) promote the proliferation and angiogenesis of tumor cells via the paracrine mechanism in vitro. Conditioned media (CM) of ovarian TAFs and normal fibroblasts (NFs) were collected. Ovarian cancer cells (OCCs) were treated with 2 mL TAFs-CM and NFs-CM in experim.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.22.14
- DOI:
- 10.4238/2013.November.22.14
Genetic variants of the endothelial NO synthase gene (eNOS) may confer increased risk of sporadic congenital heart disease
Authors: K. Zhou, Y. Wang, W. Peng, J. Sun, Y.M. Qing and X.M. Mo
The endothelial NO synthase (eNOS) enzyme is expressed during the early stages of cardiogenesis and plays an important role in normal heart development. Genetic variations of eNOS G894T have been shown to influence individual susceptibility to some phenotypes of congenital heart disease (CHD) in different populations. We condu.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.16.4
- DOI:
- http://dx.doi.org/10.4238/2014.May.16.4
Efficient detection of the V600E mutation of the BRAF gene in papillary thyroid carcinoma using multiplex allele-specific polymerase chain reaction combined with denaturing high-performance liquid chromatography
Authors: R.X. Gong1, Y.P. Gong1, J. Yang1, T. Wei1, J. Li-Ling2,3 and J.Q. Zhu1
Mutations in the V-raf murine sarcoma viral oncogene homolog B1 gene (BRAF) play an important role in the pathogenesis of papillary thyroid cancer (PTC). In this study, a BRAF V600E mutation was detected in formalin-fixed and paraffin-embedded PTC samples using multiplex allele-specific polymerase chain reaction and denaturing high-performance liquid chro.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.24.11
- DOI:
- 10.4238/2013.October.24.11
Implication of polymorphisms in DNA repair genes with an increased risk of hepatocellular carcinoma
Authors: J.S. Wu, Y.P. Chen, L.C. Wang, Y.J. Yang, C.W. Deng, B.X. Hou, Z.L. He and J.X. Chen
We explored the association between 4 XRCC1 (Arg194Trp and Arg399Gln) and XPD (Asp312Asn and Lys751Gln) polymorphisms with the development and prognosis of hepatocellular carcinoma (HCC). A total of 218 cases with HCC and 277 healthy controls were included in the study. Genotyping of the XRCC1 (Arg194Trp and Arg399Gln) and XPD.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.16.5
- DOI:
- http://dx.doi.org/10.4238/2014.May.16.5
A novel TET2 mutation in a patient with refractory cytopenia with multilineage dysplasia
Authors: D.F. Coutinho, C. Diniz, R.L.D. Filgueiras, R.L.R. Baptista,J.P. Ayres-Silva, B.C.R. Monte-M�³r, M.H. Bonamino, I.R. Zalcberg
Myelodysplastic syndrome diagnosis of karyotypically normal patients may be elusive because it relies exclusively on morphological and clinical data. In routine practice, finding of an acquired mutation or a cytogenetic abnormality provides irrefutable evidence of the clonal nature of that disease. Recurrent deletions and soma.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.22.13
- DOI:
- 10.4238/2013.November.22.13
Analysis of sequence diversity through internal transcribed spacers and simple sequence repeats to identify Dendrobium species
Authors: Y.T. Liu, R.K. Chen, S.J. Lin, Y.C. Chen, S.W. Chin, F.C. Chen and C.Y. Lee
The Orchidaceae is one of the largest and most diverse families of flowering plants. The Dendrobium genus has high economic potential as ornamental plants and for medicinal purposes. In addition, the species of this genus are able to produce large crops. However, many Dendrobium varieties are very similar in outward appearance.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.8.15
- DOI:
- 10.4238/2014.April.8.15
High-sensitivity cardiac troponin T is a predictor of recurrent acute coronary syndrome in patients with acute myocardial infarction
Authors: S.-C. Sheng, X.-M. Lu, Y. Kang, Y.-P. Du and G.-X. Wang
High-sensitivity cardiac troponin T is a useful tool for diagnosing myocardial ischemia. However, its role in the prognosis of patients with acute myocardial infarction has not been studied. Here, the prognostic value of high-sensitivity cardiac troponin T for patients with acute myocardial infarction was investigated. The con.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.16.6
- DOI:
- http://dx.doi.org/10.4238/2014.May.16.6