Analysis of differential selective forces acting on the coat protein (P3) of the plant virus family Luteoviridae
Authors: Marina W. Torres, R�©gis L. Corr�ªa and Carlos G. Schrago
The coat protein (CP) of the family Luteoviridae is directly associated with the success of infection. It participates in various steps of the virus life cycle, such as virion assembly, stability, systemic infection, and transmission. Despite its importance, extensive studies on the molecular evolution of this protein are lack.. Read More»
- Genet. Mol. Res. 4(4):
Effects of sodium lactate Ringerâ��s injection on transfection of human protein kinase C-�± antisense oligonucleotide in A549 lung cancer cells
Authors: Z.H. Wang, W.W. Sun, Y.L. Han and Z. Ma
In the present study, we evaluated the effects of four solutions [Dulbecco’s modified Eagle’s medium (DMEM), sodium lactate Ringer’s injection (SLRI), phosphate-buffered saline (PBS), and NaCl] on the transfection of the human protein kinase C-a antisense oligonucleotide (PKC-a ASO) aprinocarsen in human lung carcinoma A549 cells. Specif.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15037650
- DOI:
- 10.4238/gmr.15037650
A genomic-scale search for regulatory binding sites in the integration host factor regulon of Escherichia coli K12
Authors: M. Trindade dos Santos and Paulo S�©rgio Rodrigues
We examined general aspects of the DNA-protein interaction between the integration host factor (IHF) global regulator and its regulatory binding sites in the Escherichia coli K12 genome. Two models were developed with distinct weight matrices for the regulatory binding sites recognized by IHF. Using these matrices we performed.. Read More»
- Genet. Mol. Res. 4(4):
DNA repair gene polymorphisms and susceptibility to familial breast cancer in a group of patients from Campinas, Brazil
Authors: Rozany Mucha Dufloth, Sandra Costa, Fernando Schmitt and Luiz Carlos Zeferino
Several studies have reported that the genes involved in DNA repair and in the maintenance of genome integrity play a crucial role in protecting against mutations that lead to cancer. Epidemiologic evidence has shown that the inheritance of genetic variants at one or more loci results in a reduced DNA repair capacity and in an.. Read More»
- Genet. Mol. Res. 4(4):
Composition of constitutive heterochromatin of Pseudonannolene strinatii Mauri�¨s, 1974 (Diplopoda, Spirostreptida) analyzed by AT/CG specific fluorochromes
Authors: Kleber Agari Campos and Carmem Silvia Fontanetti
Triple staining with fluorochromes (DA/DAPI/CMA) and C-banding were used to characterize the composition of Pseudonannolene strinatii heterochromatin. C-banding showed C+ bands of different labeling intensity on chromosomes 1 and 2 in some cells. Fluorochrome staining revealed DAPI+ regions corresponding to the C-banding patte.. Read More»
- Genet. Mol. Res. 4(4):
An integrated model for cellular analysis
Authors: Eduardo Battistella, Jos�© G.C. de Souza, Cl�¡udia K. Barcellos, Ney Lemke and Jos�© C.M. Mombach
We present the MOlecular NETwork (MONET) ontology as a model to integrate data from different networks that govern cell function. To achieve this, different existing ontologies were analyzed and an integrated ontology was built in a way to make it possible to share and reuse knowledge, support interoperability between systems,.. Read More»
- Genet. Mol. Res. 4(3):
Genetic characterization of European-Zebu composite bovine using RFLP markers
Authors: Erica Perez Marson, JosÃ?© Bento Sterman Ferraz, FlÃ?¡vio Vieira Meirelles, JÃ?ºlio CÃ?©sar de Carvalho Balieiro, Joanir Pereira Eler, LuÃ?Âs Gustavo Girardi Figueiredo and Gerson Barreto MourÃ?£o
A population of 370 European-Zebu composite beef heifers, consisting of six different breed compositions (A-F), were characterized genetically, using RFLP markers of luteinizing hormone receptor (LHR) and follicle-stimulating hormone receptor (FSHR) genes. Our objectives were to genetically characterize this population and to .. Read More»
- Genet. Mol. Res. 4(3):
Temporal and spatial expression of Smads and their correlation with YAP1 expression in sheep
Authors: W. Gao, R. S, W. Sun, Q.Z. Wang, X.Y. Lv, J.J. Bao, J.R. Yu, L.H. Wang, H.H. Musa and L. Chen
The temporal and spatial patterns of Smad and Yes-associated protein 1 (YAP1) expression were investigated in skeletal muscle (gastrocnemius muscle and extensor digitorum longus) at different growth stages (2 days old, 2 and 6 months old) in Hu sheep. Smads were differentially expressed in sheep skeletal muscle, with high expression in the gastrocnemius m.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15037715
- DOI:
- 10.4238/gmr.15037715
Correlation of the TCF7L2 (rs7903146) polymorphism with an enhanced risk of type 2 diabetes mellitus: a meta-analysis
Authors: Y. Guan, L.H. Yan, X.Y. Liu, X.Y. Zhu, S.Z. Wang and L.M. Chen
Increasing evidence has demonstrated that a transcription factor 7-like 2 (TCF7L2) polymorphism (rs7903146) is significantly associated with type 2 diabetes mellitus (T2DM); however, limited sample size and variance of ethnicity in the studies investigating this association have led to conflicting reports regarding its role. Therefore, a comprehensive met.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15037969
- DOI:
- 10.4238/gmr.15037969
Improved protocol for extracting genomic DNA from frozen formalin-fixed tissue resulting in high-quality whole mtDNA
Authors: H.W. Wang, Y. Xu, H.F. Zhang, Y.J. Zeng, L. Ren, Y.L. Miao, H.Y. Luo and K.H. Wang
Formalin fixation and paraffin embedding is widely used for convenient and long-term storage of tumor tissue and precious sources to perform genetic studies. However, DNA fragmentation is one of the major flaws of genomic DNA isolation from formalin fixation tissues, which limits its further usage. Here, we present an improved method for isolating high-qu.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15037972
- DOI:
- 10.4238/gmr.15037972