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Composition of constitutive heterochromatin of Pseudonannolene strinatii Mauri�¨s, 1974 (Diplopoda, Spirostreptida) analyzed by AT/CG specific fluorochromes

Author(s): Kleber Agari Campos and Carmem Silvia Fontanetti

Triple staining with fluorochromes (DA/DAPI/CMA) and C-banding were used to characterize the composition of Pseudonannolene strinatii heterochromatin. C-banding showed C+ bands of different labeling intensity on chromosomes 1 and 2 in some cells. Fluorochrome staining revealed DAPI+ regions corresponding to the C-banding pattern, indicating that the heterochromatin of this species is abundant in AT-rich sequences.