Sexing single bovine blastomeres using TSPY gene amplification
Authors: M.C.A. Carneiro, P.L. Takeuchi, A. Ara�ºjo, R.B. L�´bo, F.P. Elias, R.A. Vila, C.L. Miranda-Furtado and E.S. Ramos
The testis-specific protein Y-encoded gene (TSPY) is a Y-specific gene present in variable copy number in many mammalian species, including cattle. We tested the applicability of the TSPY gene as a Y-specific marker to predict preimplantation embryo sex in Nelore (Bos indicus) cattle. Two blastomeres were removed from each emb.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.25.1
- DOI:
- 10.4238/2011.October.25.1
Evolution of CD4+CD25hi T cell subsets in Aspergillus-infected liver transplantation recipients reduces the incidence of transplantation rejection via upregulating the production of anti-inflammatory cytokines
Authors: T. Xing, L. Zhong, G. Qiu, L. Huang and Z. Peng
Infection is the leading risk factor of liver transplantation-related death. Aspergillosis is a life-threatening complication in immune-compromised patients, and is the cause of approximately 2/3 of deaths in liver transplant recipients. In our previous studies, we found a regulatory T cell (Treg) population that showed signif.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.4.7
- DOI:
- 10.4238/2014.July.4.7
Isolation and characterization of microsatellite loci in Sisyrinchium (Iridaceae) and cross amplification in other genera
Authors: R.B. Miz, L.O. Tacuati�¡, F.W. Cidade, A.P. de Souza, F. Bered, L. Eggers and T.T. de Souza-Chies
Recent phylogenetic studies on Sisyrinchium strongly suggest that species classified in section Hydastylus and section Viperella belong to a single group of plants in recent adaptive radiation (Clade IV). These species neither present clear morphological differentiation among them nor show clear identification using DNA barcode markers. Thus, the main goa.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038474
- DOI:
- 10.4238/gmr.15038474
Genetic dissimilarity of putative gamma-ray-induced �Preciosa - AAAB-Pome type� banana (Musa sp) mutants based on multivariate statistical analysis
Authors: R.K.N. Pestana, E.P. Amorim, C.F. Ferreira, V.B.O. Amorim, L.S. Oliveira, C.A.S. Ledo and S.O. Silva
Bananas are among the most important fruit crops worldwide, being cultivated in more than 120 countries, mainly by small-scale producers. However, short-stature high-yielding bananas presenting good agronomic characteristics are hard to find. Consequently, wind continues to damage a great number of plantations each year, leadi.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.25.2
- DOI:
- 10.4238/2011.October.25.2
A glimpse of the endophytic bacterial diversity in roots of blackberry plants (Rubus fruticosus)
Authors: M. Contreras1, P.D. Loeza2, J. Villegas1, R. Farias1 and G. Santoyo1
The aim of this study was to explore the diversity of culturable bacterial communities residing in blackberry plants (Rubus fruticosus). Bacterial endophytes were isolated from plant roots, and their 16S rDNA sequences were amplified and sequenced. Our results show that the roots of R. fruticosus exhibit low colony forming units of bacterial endophytes pe.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038542
- DOI:
- 10.4238/gmr.15038542
Mitochondrial gene expression in the liver and muscle of high and low feed efficiency Japanese quail layers subjected to different environmental temperatures
Authors: D.M. Voltolini, A.P. Del Vesco, E. Gasparino, S.E.F. Guimar�£es, A.R. Oliveira Neto, E. Batista and A.P.S. Ton
We evaluated the adenine nucleotide translocator (ANT) and cytochrome oxidase subunit III (COX III) mRNA expressions in the muscle and liver of Japanese quails presenting high and low feed efficiency (FE), and subjected them to three different environmental temperatures: comfort, heat stress (38°C), and cold stress (10°.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.4.8
- DOI:
- 10.4238/2014.July.4.8
A novel DDX5 gene in the freshwater crayfish Cherax quadricarinatus is highly expressed during ontogenesis and spermatogenesis
Authors: D.-A. Fang, Q. Wang1, J. Wang, L. He, L.-H. Liu and Y. Wang
The freshwater crayfish Cherax quadricarinatus, originally from Australia, is an invasive species that is also widely used in aquaculture. DEAD-box helicase family genes are found throughout evolution and encode RNA-binding proteins. The human DDX5 (p68) is important for normal cell growth, differentiation and proliferation. W.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.25.3
- DOI:
- 10.4238/2011.October.25.3
Cloning and sequence analysis of an actin gene in aloe
Authors: S.S. Wen, D.W. He, C.M. Liao, J. Li, G.Q. Wen, and X.H. Liu
Aloe (Aloe spp), containing abundant polysaccharides and numerous bioactive ingredients, has remarkable medical, ornamental, calleidic, and edible values. In the present study, the total RNA was extracted from aloe leaf tissue. The isolated high-quality RNA was further used to clone actin gene by using reverse transcr.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.4.9
- DOI:
- 10.4238/2014.July.4.9
Associations of serotonin receptor gene HTR3A, HTR3B, and HTR3A haplotypes with bipolar disorder in Chinese patients
Authors: J. Jian, C. Li, J. Xu, D. Qiao, G. Mi, X. Chen and M. Tang
Single nucleotide polymorphisms (SNPs) in HTR3A and HTR3B have been reported to be associated with bipolar disorder in European and Japanese populations. We explored the roles of 21 tag SNPs in HTR3A and HTR3B in susceptibility to bipolar disorder in a Chinese cohort. Twenty-one Tag SNPs were genotyped in a study consisting of 130 patients with bipolar di.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038671
- DOI:
- 10.4238/gmr.15038671
Microarray analysis in caudal medulla of cattle orally challenged with bovine spongiform encephalopathy
Authors: L.M. Almeida, U. Basu, J.L. Williams, S.S. Moore and L.L. Guan
Bovine spongiform encephalopathy (BSE) is a fatal disorder in cattle characterized by progressive neurodegeneration of the central nervous system. We investigated the molecular mechanisms involved in neurodegeneration during prion infection through the identification of genes that are differentially expressed (DE) between expe.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.25.5
- DOI:
- 10.4238/2011.October.25.5