Development of novel and polymorphic microsatellite DNA loci from Haliotis ovina
Authors: Z.B. Li, X.J. Tian, J.B. Shangguan, Y.Y. Cao, G.L. Zhang and L.N. Chen
Twelve microsatellite loci were developed from Haliotis ovina by the magnetic bead hybridization method. Genetic variability was assessed using 30 individuals from 3 wild populations. The number of alleles per locus ranged from 2 to 5, and the polymorphism information content ranged from 0.1228 to 0.6542. Observed and expected heterozygosities ranged from.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.26.6
- DOI:
- 10.4238/2015.January.26.6
Transformation of the CmACS-7 gene into melon (Cucumis melo L.) using the pollen-tube pathway
Authors: H.J. Zhang and F.S. Luan
The aim of the present study was to develop a transformation system that may be useful for introducing agronomically and biotechnologically relevant traits into melon. The production of transplanted melon with maternal inheritance of the transgene could solve problems related to outcrossing between genetically modified crops and conventional crops or thei.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038067
- DOI:
- 10.4238/gmr.15038067
mRNA and protein expression levels of four candidate genes for ear size in Erhualian and Large White pigs
Authors: L.C. Zhang, J. Liang, L. Pu, Y.B. Zhang, L.G. Wang, X. Liu, H. Yan, L.X. Wang
Porcine ear size is an important characteristic for distinguishing among pig breeds. In a previous genome-wide association study of porcine ear size, LEM domain-containing 3 (LEMD3), methionine sulfoxide reductase B3 (MSRB3), high mobility group AT-hook 2 (HMGA2), and Wnt inhibitory factor 1 (WIF1) were implicated as important.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029252
- DOI:
- 10.4238/gmr16029252
Identification and evaluation of endogenous control genes for use in quantitative RT-PCR during wheat (Triticum aestivum L.) grain filling
Authors: D. Wu, J. Dong, Y.J. Yao, W.C. Zhao and X. Gao
The use of appropriate reference genes is essential for the generation of accurate and biologically meaningful results from quantitative real-time PCR (qRT-PCR) analysis. However, studies have found that the expression of most commonly used reference genes is not always independent of the tissues, treatments, or developmental stages studied. geNormPlus, N.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.8.15
- DOI:
- http://dx.doi.org/10.4238/2015.September.8.15
Bioinformatic analyses of GRAS genes in Betula kirghisorum based on transcriptome data
Authors: C.J. Yang, G.Y. Li and Y.L. Cui
The transcriptomes of salt-stressed and unstressed Betula kirghisorum plants were analyzed using high throughput sequencing technology. A total of 52,239,804 and 51,772,998 clean reads were obtained from the two libraries, respectively, and de novo assembled into 60,545 all-unigenes. A total of 39,997 unigenes were annotated using public databases. Overal.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038321
- DOI:
- 10.4238/gmr.15038321
Aspergillus nidulans as a biological system to detect the genotoxic effects of mercury fumes on eukaryotes
Authors: G.D. Sousa, T.D. Zucchi, F.D. Zucchi, R.G. Miller1, R.M.A. Anjos,P. Poli and T.M.A.D. Zucchi
Mercury (Hg) pollution is one of the most serious environmental problems. Due to public concern prompted by the symptoms displayed by people who consumed contaminated fish in Minamata, Japan in 1956, Hg pollution has since been kept under constant surveillance. However, despite considerable accumulation of knowledge on the nox.. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2gmr587
- DOI:
- 10.4238/vol8-2gmr587
Efficient identification of ornamental peach cultivars using RAPD markers with a manual cultivar identification diagram strategy
Authors: J. Han, W.Y. Wang, X.P. Leng, L. Guo, M.L. Yu, W.B. Jiang and R.J. Ma
One of the most important uses of DNA markers is cultivar identification. However, no DNA fingerprint analysis strategy is available for making DNA markers helpful in practical plant cultivar identification, especially for the identification of a large number of cultivars. We developed a manual cultivar identification diagram .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.8.2
- DOI:
- 10.4238/2014.January.8.2
Effects of sacral nerve stimulation with acupuncture on gut transit time and c-kit expression in colon of rats with slow transit constipation
Authors: Y.G. Zhang, W.J. Shao, Y.F. Gu, J.F. Qiu, L. Yuan and G.D. Li
Sacral nerve stimulation (SNS) is an alternative surgical approach to alleviate fecal incontinence and constipation. This study aimed to explore the effects and underlying mechanisms of SNS with acupuncture on gut transit time and colon c-kit protein expression in rats with slow transit constipation (STC). Fifty Sprague-Dawley rats were randomly divided i.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038362
- DOI:
- 10.4238/gmr.15038362
Effects of ABCB1 3435C>T genotype on serum levels of cortisol and aldosterone in women with normal menstrual cycles
Authors: T. Nakamura, N. Okamura, M. Yagi, H. Omatsu, M. Yamamori,A. Kuwahara, K. Nishiguchi, M. Horinouchi, K. Okumura and T. Sakaeda
ABCB1, also known as MDR1/P-glycoprotein, can transport cortisol and aldosterone. We examined the effects of ABCB1 polymorphisms on serum levels of cortisol and aldosterone among different phases of the normal menstrual cycle in 51 non-pregnant healthy Japanese female volunteers (22 ± 1 years old). The menstrual cycle w.. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2gmr574
- DOI:
- 10.4238/vol8-2gmr574
Characterization of RsMYB28 and RsMYB29 transcription factor genes in radish (Raphanus sativus L.)
Authors: X.B. Luo, Z. Liu, L. Xu, Y. Wang, X.W. Zhu, W. Zhang, W. Chen, Y.L. Zhu, X.J. Su, M. Everlyne and L.W. Liu
Glucosinolates (GSLs) are important secondary metabolites in Brassicaceae plants. Previous studies have mainly focused on GSL contents, types, and biosynthesis-related genes, but the molecular characterization patterns of GSL biosynthesis-related transcription factors remain largely unexplored in radish (Raphanus sativus L.). To isolate transcription fact.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038381
- DOI:
- 10.4238/gmr.15038381