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Animal Genetics   Research Article

Isolation and characterization of microsatellite loci in Branchiostoma belcheri Gray (Amphioxus)

Authors: Z.B. Li, Y.S. Huang, J.B. Shangguan, Y.F. Ning, Y. Yi and G. Dai

Branchiostoma belcheri Gray is a second-class, nationally protected protochordate in China. We developed 10 novel polymorphic sites in B. belcheri, which were examined using a population of 30 wild individuals from Xiamen, China. The polymorphism information content ranged between 0.141 and 0.681, and the number of alleles varied from 2 to 5. The expected.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.August.28.5
DOI:
http://dx.doi.org/10.4238/2015.August.28.5
Microbial Genetics   Research Article

Isolation and characterization of microsatellite loci for Cunninghamia lanceolata (Lamb.) Hook

Authors: Y.X. Li, Z.S. Wang, J.K. Sui, Y.F. Zeng, A.G. Duan and J.G. Zhang

As a result of human activities, wild populations of Cunninghamia lanceolata (Cupressaceae) have sharply declined in recent years. The development and implementation of a valid conservation strategy require a clear understanding of the genetic makeup of this species. Eleven polymorphic microsatellite loci were isolated and characterized from samples of 52.. Read More»

Genet. Mol. Res. 14(1):
2015.January.23.19
DOI:
10.4238/2015.January.23.19
Animal Genetics   Research Article

Differential expression of peroxisome proliferator-activated receptor �³, fatty acid synthase, and hormone-sensitive lipase in fat-tailed and thin-tailed sheep breeds

Authors: X.C. Xu, B.B. Li, X. Wei, Y.X. Yang, X.L. Wang, Y.L. Chen

Tail fat content affects meat quality, and it varies in different sheep breeds. Theoretically, lipid metabolism contributes to variation in tail fat content. Tail length, tail width, and tail girth were measured in live Tong sheep (with both short fat tail and long fat tail), Shaanbei fine wool sheep (long thin tail), Tan shee.. Read More»

Genet. Mol. Res. 14(4):
Animal Genetics   Research Article

Duplication polymorphisms in exon 4 of �º-casein gene in yak breeds/populations

Authors: S. Pingcuo, J. Gao, Z.R. Jiang, S.Y. Jin, C.Y. Fu, X. Liu, L. Huang and Y.C. Zheng

The objective of this study was to compare 12 bp-duplication polymorphisms in exon 4 of the κ-casein gene among 3 breeds/populations of yak (Bos grunniens). Genomic DNA was extracted from yak blood or muscle samples (N = 211) and a partial sequence of exon 4 of κ-casein gene was amplified by polymerase chain reaction. A polyacrylamide gel elec.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.August.28.8
DOI:
http://dx.doi.org/10.4238/2015.August.28.8
Human Genetics   Research Article

Inheritance of balanced translocation t(17; 22) from a Down syndrome mother to a phenotypically normal daughter

Authors: X.Y. Liu, Y.T. Jiang, R.X. Wang, L.L. Luo, Y.H. Liu and R.Z. Liu

We report that a 30-year-old woman with mental retardation was referred for prenatal diagnoses during pregnancy. An ultrasound scan showed that the heart structure and function of the fetus were normal. Cytogenetic analysis showed that the female karyotype was 47,XX, t(17; 22) (q21; q11), +21. The woman’s husband had a normal male karyotype and was .. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.August.28.11
DOI:
http://dx.doi.org/10.4238/2015.August.28.11
Human Genetics   Research Article

Integrated microRNA-mRNA analysis of pancreatic ductal adenocarcinoma

Authors: P.F. Liu, W.H. Jiang, Y.T. Han, L.F. He, H.L. Zhang and H. Ren

The main aim of this study was to explore the underlying molecular mechanisms and potential target molecules of pancreatic adenocarcinoma. The miRNA (GSE32678) and mRNA (GSE32676) expression profiles of patients with pancreatic ductal adenocarcinoma and healthy controls were downloaded from the Gene Expression Omnibus database. Differentially expressed mi.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.August.28.14
DOI:
http://dx.doi.org/10.4238/2015.August.28.14
Animal Genetics   Research Article

Characterization of a novel CAPN3 transcript generated by alternative splicing in cattle

Authors: S.Y. Liu, H. Jiang, B. Yuan, Y. Gao, L.S. Dai and J.B. Zhang

Calpain-3 (CAPN3) is a member of the calpain family of Ca2+-regulated cysteine proteases, which play an important role in sarcomere remodeling and mitochondrial protein turnover, and thus, regulating beef tenderness in cattle. Currently, multiple CAPN3 transcripts have been detected in human, monkey, rat, and rabbit. However, whether this transcript is pr.. Read More»

Genet. Mol. Res. 14(1):
2015.January.23.20
DOI:
10.4238/2015.January.23.20
Medical Genetics   Research Article

Inhibitory effects of spironolactone on myocardial fibrosis in spontaneously hypertensive rats

Authors: H. Zhao, D.W. Gu, H.T. Li, Q.F. Ge and G.P. Li

This study evaluated the inhibitory effects of spironolactone, a non-selective aldosterone receptor antagonist, on hypertension-induced myocardial fibrosis. Collagen I and III contents was detected in the myocardial tissue of spontaneously hypertensive rats (SHRs) after spironolactone administration. Twenty male SHRs were assigned to the spironolactone gr.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.August.28.17
DOI:
http://dx.doi.org/10.4238/2015.August.28.17
Human Genetics   Research Article

Expression and clinical significance of CD4+ CD45+ peripheral blood T cells in patients with ulcerative colitis

Authors: L.Y. Guo, Y.R. Li and X.P. Jin

The objectives of this study were to explore the expression of peripheral blood CD4+CD45+ T cells in patients with ulcerative colitis (UC) and determine its clinical value. We selected 80 patients with UC from the First Affiliated Hospital of Liaoning Medical University from March 2012 to December 2013. Of these, 27 had mildly active, 28 moderately active.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.August.28.20
DOI:
http://dx.doi.org/10.4238/2015.August.28.20
Microbial Genetics   Research Article

First transcriptional survey of the Malpighian tubules of giant mealworm, Zophobas morio (Coleoptera: Tenebrionidae)

Authors: J.R. Silva, R.A. Prado, D.T. Amaral and V.R. Viviani

The Malpighian tubules play a key role in insect osmoregulation. Although a transcriptional analysis has been done for the Malpighian tubules in Drosophila melanogaster (Diptera), no functional genomics analysis has yet been carried out for any Coleoptera species. Recently, we constructed a cDNA library from Malpighian tubules of larval Zophobas morio, a .. Read More»

Genet. Mol. Res. 14(1):
2015.January.23.21
DOI:
10.4238/2015.January.23.21