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Medical Genetics   Research Article
Comparative analysis of genomic DNA extraction protocols: Maxi-preparation of quality DNA for genetic evaluation and phylogenetic studies

Authors: Muhammad Naeem, Shoaib Hassan, Samra Masud, Qurban Ali, Sikandar Hayat, Ammar Danyal Naeem and Zara Naeem

Four different DNA extraction protocols were compared for ability to produce DNA from the various tissues (caudal fin, pectoral fin, pelvic fin, muscle and gills) of three different freshwater fish species (Hypophthalmichthys molitrix, Labeo rohita and Sperata sarwari). Study describes the comparison of Phenol-chloroform; salt extraction; TNES and Urea me.. Read More»

Genet. Mol. Res. 19(1):

Human Genetics   Research Article
Hepatitis B and C prevalence and its associated risk factors among school going children in urban and rural areas of District Muzaffar Garh, Pakistan

Authors: Sikandar Hayat, Muhammad Naeem, Arif Malik, Qurban Ali, Muhammad Khalid, Samrah Masud and Arif Mahmood Bhutta

Viral Hepatitis like hepatitis B and hepatitis C are the serious cause of mortality in Pakistan. Present study estimated the prevalence and risk factors associated with Hepatitis B and C among school going children from urban and rural areas. In urban areas overall prevalence of HBV was 5% where the highest prevalence of HBV was 80% and 20% in males and f.. Read More»

Genet. Mol. Res. 19(1):

Plant Genetics   Review Article
Fusarium wilt’s pathogenic studies and disease management: A review

Authors: Ch. Muhammad Rafiq, Muhammad Tariq Mahmood, Mushtaq Ahmad, Imtiaz Ali, Sadia Kaukab, Muhammad Shafiq, Muhammad Saleem, Umer Iqbal

Fusarium wilt caused by Fusarium oxysporum f. sp. ciceris is one of the most important fungal diseases of chickpea limiting its productivity in almost all chickpea growing countries across the world. F. oxysporum is soil borne and root inhabiting in nature which may survives in soil for longer period (up to six years). Pathogen undergoes asexual reproduct.. Read More»

Genet. Mol. Res. 19(1):

Plant Genetics   Research Article
Genetic studies for yield and related traits in micro and macrosperma exotic lentil genotypes

Authors: Ch. Muhammad Rafiq, Sadia Kaukab, Aqsa Tahir, Masood Qadir, Busharat Hussain, Shakeel Ahmad Anwar, Muhammad Tariq Mahmood, Ali Aziz, Amir Hameed and Khalid Mehmood Mughal

With respect to seed size lentil (Lens culinaris Medikus) species usually are distributed into two main groups namely Microsperma (<2.5 g/100-seeds) with small rounded seeds and Macrosperma (>2.5 g/100-seeds) with larger and more flattened seeds. The seeds/pod and pods/plant will decrease as the seed size increases that adversely affect the seed yie.. Read More»

Genet. Mol. Res. 19(1):

Animal Genetics   Research Article
Uncoupling Proteins and Cardiac Apoptosis in High Fat Diet-Fed Male Rats

Authors: K. Alsolami, Z. Alrefaie

This study aimed to assess impact of high fat diet (HFD) on cardiac apoptosis, and cardiac uncoupling proteins (UCPs) 2&3 expression. Twenty rats were fed either (45%) or (10%) fat diet for 6 months, then cardiac tissue expression of BAX, Bcl2, Fas, Fas-L (markers for apoptotic pathways) and UCP 2&3 were assessed. Results revealed enhancement of i.. Read More»

Genet. Mol. Res. 19(1):

Plant Genetics   Research Article
Identification and Characterization of Δ1-pyrroline-5-carboxylate Synthetase in Lepidium draba Plant and Investigating the Expression Level of this Gene and Some Biochemical Traits under Salt Stress

Authors: Rambod Pakzad, Kiarash Jamshidi Goharrizi, Ali Riahi-Madvar, Farzane Amirmahani, Mojtaba Mortezavi, Leila Esmaeeli

Lepidium draba, a medicinal herb belongs to the Brassicaceae family is more considered because it contains high level of glucoraphanin, as a precursor of sulforaphane (an anti-carcinogenic and anticancer agent). Whilst there has been studies on plant response to salinity stress, to date the response of L. draba to salinity stress is poorly understood. The.. Read More»

Genet. Mol. Res. 19(1):