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Microbial Genetics   Research Article
A Xanthomonas citri subsp citri hypothetical protein related to virulence contains a non-functional HD domain and is implicated in flagellar motility

Authors: F.C.F. Vieira, A.M. Gon�§alves, E.F.R. Mendoza, R.M. Ferreira, M.L.M. Costa, T.S. Balbuena, H.G. Sebinelli, P. Ciancaglini, J.M. Pizauro Junior and J.A. Ferro

Citrus canker, caused by the Gram-negative bacterium Xanthomonas citri subsp citri (Xac), severely affects most economically important citrus varieties worldwide. A previous study showed that disruption of the ORF XAC1201 from the Xac 306 strain by transposon Tn5 decreased bacterium virulence in the Rangpur lime host (Citrus l.. Read More»

Genet. Mol. Res. 16(3): gmr16039728

DOI: 10.4238/gmr16039728

Plant Genetics   Research Article
ITS2 as a molecular marker for the identification of Diatraea saccharalis and D. flavipennella and possible infection with Cotesia spp

Authors: M.S. Regueira Neto, R.P. Barros, M.A.J. Morais, V.Q. Balbino and V. Loreto

In Brazil, the species Diatraea flavipennella and D. saccharalis play an important role in the sugar and alcohol agribusiness by causing many damages in sugarcane fields. The egg, larva, pupa, and adult stages are very morphologically similar between these species, and the identification can be confused. The internal transcribed spacer 2 (ITS 2) from ribo.. Read More»

Genet. Mol. Res. 16(3): gmr16039732

DOI: 10.4238/gmr16039732

Plant Genetics   Research Article
Diallel and prediction (REML/BLUP) for yield components in intervarietal maize hybrids

Authors: I.R. Carvalho, A.J. de Pelegrin, V.J. Szareski, M. Ferrari, T.C. da Rosa, T.S. Martins, N.L. dos Santos, M. Nardino, V.Q. de Souza, A.C. de Oliveira and L.C. da Maia

Genetic improvement is essential to achieve increments in maize (Zea mays L.) grain yield components. It may be obtained through crosses, which enable to exploit the effects of intervarietal heterosis, allelic complementarity, as well as gene actions and effects. This study estimated the components of variance and genetic para.. Read More»

Genet. Mol. Res. 16(3): gmr16039734

DOI: 10.4238/gmr16039734

Plant Genetics   Research Article
Analysis of genetic variability of commercial melon cultivars using SSR molecular markers

Authors: N. Carvalho, F.M. Canela, P.H.S. Leite, M.A. Ferreira, V.R. Oliveira, M.F. Santos, N.O.S. Souza and G.S.C. Buso

Taking into account the scenario of melon production (Cucumis melo L.) in Brazil, it is notable the importance of the culture in the fruit market. The preference of the national market is concentrated in the fruits of the yellow type. Therefore, it is possible that the genetic base of the commercial cultivars is relatively nar.. Read More»

Genet. Mol. Res. 16(3): gmr16039739

DOI: 10.4238/gmr16039739

Human Genetics   Research Article
Mutational screening in the LDLR gene among patients presenting familial hypercholesterolemia in the Southeast of Brazil

Authors: G.A. Molfetta, D.L. Zanette, J.E. Santos and W.A. Silva Jr

Familial hypercholesterolemia (FH) is a dominant, autosomal disease characterized by high LDL levels in blood plasma, and is caused by a defect in the gene encoding the LDL receptor (LDLR). The clinical diagnosis is based on personal and familial history, physical examination findings, and measures of high LDL cholesterol conc.. Read More»

Genet. Mol. Res. 16(3): gmr16039226

DOI: 10.4238/gmr16039226

Plant Genetics   Research Article
Morphological diversity of cassava accessions of the south-central mesoregion of the State of Mato Grosso, Brazil

Authors: B.W. Zago, M.A.A. Barelli, E.S.S. Hoogerheide, C.L. CorrĂ?ÂȘa,G.I.S. Delforno and C.J. da Silva

Genetic variability of cassava (Manihot esculenta Crantz) in Brazil is wide, being this the result of natural and cultural selection during pre- and post-domestication of the species in different environments. Given the number of species of the genus found in the region (38 of a total of 98 species), the central region of Braz.. Read More»

Genet. Mol. Res. 16(3): gmr16039725

DOI: 10.4238/gmr16039725

Plant Genetics   Research Article
Internal amplification control of PCR for the Glu1-Dx5 allele in wheat

Authors: H.N. Heim, E.S.N. Vieira, L.R.T. Polo, N.K. Lima, G.J. Silva, G.A. Linde, N.B. Colauto and I. Schuster

One of the limiting factors in using dominant markers is the unique amplification of the target fragment. Therefore, failures in polymerase chain reaction (PCR) or non-amplifications can be interpreted as an absence of the allele. The possibility of false negatives implies in reduced efficiency in the selection process in gene.. Read More»

Genet. Mol. Res. 16(3): gmr16039718

DOI: 10.4238/gmr16039718

Human Genetics   Research Article
MiR-200c inhibits metastasis of breast tumor via the downregulation of Foxf2

Authors: T. Zhang, J.G. Wan, J.B. Liu and M. Deng

The forkhead box F2 (Foxf2) gene suppresses epithelial-mesenchymal transition via the modulation of transcription of zinc finger E-box-binding homeobox 1 (Zeb1) and epithelial (E)-cadherin, thereby inhibiting tumor metastasis. Additionally, the specific binding of microRNA (miR)-200c to Foxf2 mRNA impedes metastatic pulmonary .. Read More»

Genet. Mol. Res. 16(3): gmr16038971

DOI: 10.4238/gmr16038971