Z. Xiao
The effect of EPS to LPS injure RAW264.7 cell NF-κB and MAPK signaling pathway
This study effects of Echinacea polysaccharide(EPS) to Lipopolysaccharides(LPS) injure RAW264.7 cell NF-κB and MAPK signaling pathway. The test was divided into blank group、LPS group and 100 mu g/mL EPS + LPS group, The respectively extract Cytoplasm protein and nucleoprotein. After Gel electrophoresis, the Western Blot detected NF-κB, p-p65, IκB.. Read More»
Genet. Mol. Res. 16(4): gmr16039846
DOI: 10.4238/gmr16039846
Expression and serological diagnosis of Mycobacterium tuberculosis CFP-10 and Rv2626c proteins
We constructed a Mycobacterium tuberculosis vector expressing CFP-10 and Rv2626c to examine the expression of these proteins in Escherichia coli as well as their immunoreactivity. The CFP-10 and Rv2626c genes were amplified from tuberculosis H37Rv genomic DNA using polymerase chain reaction. They were ligated into the expression vector PET30a and e.. Read More»
Genet. Mol. Res. 13(3): 2014.September.12.5
DOI: 10.4238/2014.September.12.5
MTHFR C677T polymorphism and osteoporotic fracture in postmenopausal women: a meta-analysis
Numerous studies have evaluated the association between MTHFR C677T polymorphism and osteoporotic fracture risk in postmenopausal women. However, the results have been inconsistent. We performed a meta-analysis of the association between MTHFR C677T polymorphism and osteoporotic fracture risk in postmenopausal women. Only seven case-control studies were retrieved, w.. Read More»
Genet. Mol. Res. 13(3): 2014.September.12.1
DOI: 10.4238/2014.September.12.1
Expression and diagnostic value of proteins in Mycobacterium tuberculosis
We constructed a prokaryotic expression vector expressing the Mycobacterium tuberculosis protein TB16.3, as well as 3 other proteins, including TB15.3, CFP-10, and Rv2626C, which were purified and analyzed for their effectiveness as detection antibodies. The TB16.3 genes of M. tuberculosis H37Rv genomic DNA were amplified by polymerase cha.. Read More»
Genet. Mol. Res. 13(3): 2014.September.26.16
DOI: 10.4238/2014.September.26.16
Molecular cloning and characterization of a chlorophyll degradation regulatory gene (ZjSGR) from Zoysia japonica
The stay-green gene (SGR) is a key regulatory factor for chlorophyll degradation and senescence. However, to date, little is known about SGR in Zoysia japonica. In this study, ZjSGR was cloned, using rapid amplification of cDNA ends-polymerase chain reaction (PCR). The target sequence is 831 bp in length, corresponding to 276 amino acids. Protein BLAST results showe.. Read More»
Genet. Mol. Res. 15(2): gmr.15028176
DOI: 10.4238/gmr.15028176
Isolation and characterization of microsatellite loci in Quercus fabri (Fagaceae)
Quercus fabri is a pioneer species of secondary succession in evergreen broadleaved forests in China. In this study, we isolated and developed 12 polymorphic and 2 monomorphic microsatellite loci for Q. fabri using the biotin-streptavidin capture method. We characterized 12 polymorphic loci in 52 individuals from two populations. The number of alleles per locus rang.. Read More»
Genet. Mol. Res. 15(2): gmr.15028322
DOI: 10.4238/gmr.15028322
Prospective study of MTHFR genetic polymorphisms as a possible etiology of male infertility
The aim of this study was to explore the relationship between 2 genetic polymorphisms of the methylenetetrahydrofolate reductase gene (MTHFR), C677T and A1298C, and determine the long-term reproductive outcome in infertile men. This was a prospective study conducted in an andrology clinic. Men with a 1-year history of infertility were assessed for the M.. Read More»
Genet. Mol. Res. 13(3): 2014.March.24.26
DOI: 10.4238/2014.March.24.26
Combination of high-intensity focused ultrasound irradiation and hydroxyapatite nanoparticle injection to injure normal goat liver tissue in vivo without costal bone incision
The aims of this study were to evaluate the in vivo safety of intravenous nano-hydroxyapatite (nano-HA), to explore how nano-HA might influence the effects of high-intensity focused ultrasound (HIFU) on normal liver tissue, and to investigate whether intravenous nano-HA could enhance HIFU for hepatocellular carcinoma ablation in a goat model... Read More»
Genet. Mol. Res. 13(4): 2014.October.20.6
DOI: 10.4238/2014.October.20.6
Cloning and expression of the 1-aminocyclopropane-1-carboxylic oxidase gene from Agrostis stolonifera
A gene encoding 1-aminocyclopropane-1-carboxylic oxidase (ACO), which catalyzes the terminal step in ethylene biosynthesis, was isolated from Agrostis stolonifera. The AsACO gene is composed of 975 bp, encoding 324 amino acids. Three exons interspersed by two introns form AsACO gDNA. A BLAST search of the nucleotide sequence revealed a high level of similarity (79-9.. Read More»
Genet. Mol. Res. 15(4): gmr15049034
DOI: 10.4238/gmr15049034
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