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The effect of EPS to LPS injure RAW264.7 cell NF-κB and MAPK signaling pathway

Author(s): Q.D. Jiang, X.G. Zhou, Xiao.Z, X.P. Li and F.R. Nie

This study effects of Echinacea polysaccharide(EPS) to Lipopolysaccharides(LPS) injure RAW264.7 cell NF-κB and MAPK signaling pathway. The test was divided into blank group、LPS group and 100 mu g/mL EPS + LPS group, The respectively extract Cytoplasm protein and nucleoprotein. After Gel electrophoresis, the Western Blot detected NF-κB, p-p65, IκB-α, p-IκB-α, JNK/p-JNK, ERK/p-ERK, p38/p-p38 several protein expressions. The Results showed that when LPS stimulate RAW264.7, the NF-κB, p-p65, p-IκB-α protein expression increased within the nucleus. Compared with the LPS group, and at 3h, 6h EPS group can effectively restrain increase of NF-κBp65, at 1h, 3h, 6h to restrain expression increases of NF-κB, p-p65 protein expression. At 1h, 3h, EPS group can effectively restrain decrease of IκB-α protein expression (p﹤0.05) and p-IκB-α protein expression increase (p﹤0.05) at 6h. It showed that EPS inhibiting inflammatory reaction and inhibit nuclear factor protein expression. When LPS stimulate RAW264.7, the JNK, ERK, p38 protein expression quantity obviously decreased with longer duration of action at 1h, 3h, 6h. and p-JNK, p-ERK, p-p38 protein expression quantity increased significantly. EPS group JNK, ERK, p38 protein expression were higher than in LPS group. But the p-JNK, p-ERK, p-p38 express quantity significantly reduced. It indicated that EPS inhibition of inflammatory response was associated with activation MAPK signaling pathway