X.J. Xu

A comparative study on genetic characteristics of two new varieties of Pelodiscus sinensis and their hybrid

• H.Q. Zhang
• X.J. Xu
• Z.Y. He
• J.Z. Shao
• X.H. Zhang
• Q.H. Meng
• F.Y. Huang

Chinese soft-shelled turtle Pelodiscus sinensis has been an important aquaculture species in Southeast Asian countries. To breed a new variety of soft-shelled turtle with excellent properties and to evaluate the effect of hybridization of two turtle strains with a highly different trait phenotype, inheritance, microsatellite loci, and transcriptome analysis were stu.. Read More»

Genet. Mol. Res. 16(3): gmr16039148

DOI: 10.4238/gmr16039148

Differentiation of four strains of Chinese soft-shelled turtle (Pelodiscus sinensis) based on high-resolution melting analysis of single nucleotide polymorphism sites in mitochondrial DNA

H.Q. Zhang, C. Zhang, X.J. Xu, J.J. Zhu, Z.Y. He and J.Z. Shao

The Chinese soft-shelled turtle (Pelodiscus sinensis) has been one of the most economically important aquatic animals in China for thousands of years, and several breeding strains have been formed. Since the morphological characteristics of some strains are similar, a rapid and accurate molecular method to differentiate between strains is required. In this study, partial sequences of mitochondr.. Read More»

Genet. Mol. Res. 14(4): 2015.October.26.10

DOI: 10.4238/2015.October.26.10

Intracellular survival of virulence and low-virulence strains of Vibrio parahaemolyticus in Epinephelus awoara macrophages and peripheral leukocytes

X.J. Xu, B.H. Sang, W.B. Chen, Q.P. Yan, Z.Y. Xiong, J.B. Su and W.Z. Zou

In this study, we examined the virulence factors and pathogenesis of Vibrio parahaemolyticus in Epinephelus awoara. The chemotactic motility of V. parahaemolyticus for phagocytosis and intracellular survival in fish macrophages was determined using virulence strains and low-virulence strains of V. parahaemolyticus. We found that the intracellular mean number of virulence strains of V. parahaemo.. Read More»

Genet. Mol. Res. 14(1): 2015.January.30.14

DOI: 10.4238/2015.January.30.14

Mutation analyses and prenatal diagnosis in families of X-linked severe combined immunodeficiency caused by IL2Rg gene novel mutation

Q.L. Bai, N. Liu, X.D. Kong, X.J. Xu and Z.H. Zhao

We investigated the feasibility of interleukin-2 receptor gamma (IL2Rg) gene based on gene mutation analysis and prenatal diagnosis of X-linked severe combined immunodeficiency (XSCID). Blood samples of patients and their parents of X-SCID (family 1) and X-SCID (family 2) were collected. IL2Rg gene sequences of the 2 families were analyzed using bi-directional direct sequencing by polymerase ch.. Read More»

Genet. Mol. Res. 14(2): 2015.June.9.2

DOI: 10.4238/2015.June.9.2

Mutation analysis and prenatal diagnosis for three families affected by isolated methylmalonic aciduria

X.D. Kong1, H.R. Shi1, N. Liu1, Q.H. Wu1, X.J. Xu2, Z.H. Zhao1, N. Lu1, J. Li-Ling3 and D. Luo4

Isolated methylmalonic acidemia (MMA) is a genetically heterogeneous disorder caused mainly by deficiency of methylmalonyl-CoA mutase. In the present study, we analyzed MUT gene mutations in 3 Chinese couples with a birth history of isolated MMA... Read More»

Genet. Mol. Res. 13(4):

Effect of codon optimization on expression levels of human cystatin C in Pichia pastoris

Y.M. Li, D.J. Li, X.J. Xu, M. Cui, H.H. Zhen and Q. Wang

Human cystatin C (CysC) is a cysteine proteinase inhibitor with many potential applications. To facilitate further studies of the functions and applications of CysC, we improved the heterologous expression of CysC using a basic codon optimization method. In this study, we cloned the high-GC content wild-type sequence of the CysC gene and also designed a sli.. Read More»

Genet. Mol. Res. 13(3): 2014.July.4.14

DOI: 10.4238/2014.July.4.14

Association of paraoxonase polymorphisms with carotid artery atherosclerosis in essential hypertension patients

L. Han1,2, X.J. Xu1,2, X.H. Liang1,2 and J. Ma1,2

We investigated the relationships between paraoxonase genetic polymorphisms and essential hypertension in carotid artery atherosclerotic patients. The study included 353 Han participants and 240 Uighur participants from Xinjiang; they were further divided into two groups: essential hypertension with carotid artery atherosclerosis (CAAD group) and essential hypertension without carotid artery at.. Read More»

Genet. Mol. Res. 12(4): 2013.October.30.2

DOI: 10.4238/2013.October.30.2

Genetic polymorphisms in very important pharmacogenomic (VIP) variants in the Tibetan population

T.B. Jin, X.J. Xun, X.G. Shi, D.Y. Yuan, T. Feng, T.T. Geng and L.L. Kang

Genetic polymorphisms of very important pharmacogenomic (VIP) variants are important for personalized medicine. However, these have not been extensively studied in the Tibetan population. In this study, 82 VIP variants were detected in the Tibetan and Han (HAN) populations from northwestern China. Subsequently, we compared the differences between the Tibetan populat.. Read More»

Genet. Mol. Res. 14(4): 2015.October.16.17

DOI: 10.4238/2015.October.16.17