P.S.C. Lima
Comparison of eight methods to isolate genomic DNA from Hancornia speciosa
Mangabeira (Hancornia speciosa) is a native fruit tree found mainly in the Cerrado biome and shows great economic potential due to its multiple uses; the fruits are used in agriculture, are important as a food resource, and can be consumed in natura or processed. Due to a reduction in the area of ecosystems where it occurs, mangabeira is threatened by genetic erosio.. Read More»
Genet. Mol. Res. 16(3): gmr16039724
DOI: 10.4238/gmr16039724
Investigating genetic diversity in sapucaia using inter simple sequence repeat markers
Sapucaia is a tree species originating from the Brazilian Amazon and is widely distributed in Brazil, especially in the mid-north region (Piauí and Maranhão states). Its seeds are rich in calories and proteins, and possess great potential for commercialization. Little is known about the genetic variability in the germplasm of most Lecythis species. Here, 11 inter-simple sequence r.. Read More»
Genet. Mol. Res. 15(3): gmr.15038565
DOI: 10.4238/gmr.15038565
Molecular characterization of cajá, Spondias mombin (Anacardiaceae), by RAPD markers
The arboreal species Spondias mombin L. (Anacardiaceae) is widely distributed in Brazil, where the fruits, known by the common name of cajá, are an important commercial commodity. We evaluated genetic variability among 32 cajá accessions of the Germplasm Collection of Embrapa Meio-Norte using RAPD technique. Reaction conditions for efficient RAPD ampli.. Read More»
Genet. Mol. Res. 10(4): 2011.November.25.1
DOI: 10.4238/2011.November.25.1
RAPD analysis of the genetic diversity of mango (Mangifera indica) germplasm in Brazil
We evaluated genetic variability of mango (Mangifera indica) accessions maintained in the Active Germplasm Bank of Embrapa Meio-Norte in Teresina, Piauí, Brazil, using RAPDs. Among these accessions, 35 originated from plantings in Brazil, six from the USA and one from India. Genomic DNA, extracted from leaf material using a commercial purification kit, was su.. Read More»
Genet. Mol. Res. 10(4): 2011.December.14.1
DOI: 10.4238/2011.December.14.1
Determination of the genetic diversity among accessions of Senna spectabilis (canafístula) by using RAPD markers
Senna spectabilis (DC.) H.S. Irwin & Barneby (Fabaceae; Caesalpinioideae), commonly known as “canafístula” or “cassia”, is widely used in the semi-arid region of northeastern Brazil as a source of forage and timber. The plant presents a high nutritional content in comparison with other forage species that are native to the Brazilian Caatinga; thus, it represen.. Read More»
Genet. Mol. Res. 13(4): 2013.December.2.12
DOI: 10.4238/2013.December.2.12
DNase concentration assay to obtain DNA-free RNA from sugarcane leaves
The success of gene expression studies, protein synthesis, and construction of cDNA libraries directly depends on the purity and integrity of the RNA used in these tests, as even minor amounts of contaminant DNA (<1%) can produce a false positive amplification signal in quantitative real-time PCR. For RNA contaminated with genomic DNA, an essential step in the st.. Read More»
Genet. Mol. Res. 15(4): gmr15049073
DOI: 10.4238/gmr15049073
Genetic variation detected by RAPD markers in natural populations of babassu palm (Attalea speciosa Mart.)
The purpose of this study was to analyze the effects of management on the genetic structure of natural populations of Attalea speciosa in the State of Piauí, Brazil, using randomamplified polymorphic DNA (RAPD) markers. Three babassu populations under different management systems were selected. Polymerase chain reactions were performed for 20 RAPD primers. A total of 146 bands were gener.. Read More»
Genet. Mol. Res. 14(2): 2015.June.8.10
DOI: 10.4238/2015.June.8.10
Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers
Cratylia argentea (Desv.) Kuntze (Fabaceae) is a drought-tolerant, perennial legume found primarily in Brazil, Bolivia, and Peru. The shrub is well adapted to acid soils and exhibits high productivity and nutritional value, characteristics that would favor its use as a dry season animal forage supplement in semiarid regions. In plant improvement programs, t.. Read More»
Genet. Mol. Res. 14(4): 2015.November.25.12
DOI: 10.4238/2015.November.25.12
RAPD analysis of the genetic diversity among accessions of Fabaceous forages (Poincianella spp) from the Caatinga
Among members of the Fabaceae family, native to the Brazilian Caatinga, the species Poincianella pyramidalis and P. bracteosa exhibit particular potential as forage for cattle, sheep and goats. With the aim of establishing genetic relationships within Poincianella, random amplified polymorphic DNA analysis was performed on eight accessions of P.. Read More»
Genet. Mol. Res. 13(3): 2014.August.1.1
DOI: 10.4238/2014.August.1.1
Evaluation of genetic similarity between accessions of Pityrocarpa moniliformis (angico-de-bezerro) using RAPD markers
Pityrocarpa moniliformis (Benth.) Luckow and Jobson, commonly known as angico-de-bezerro, is a forage legume that occurs naturally in the Caatinga of northeastern Brazil. This fast growing, vigorous, melliferous tree is well adapted to arid terrains and its branches and leaves possess high nutritional value. However, the scarcity of information regarding genetic variability within the .. Read More»
Genet. Mol. Res. 13(3): 2014.July.29.9
DOI: 10.4238/2014.July.29.9
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