S.E.S. Valente
Comparison of eight methods to isolate genomic DNA from Hancornia speciosa
Mangabeira (Hancornia speciosa) is a native fruit tree found mainly in the Cerrado biome and shows great economic potential due to its multiple uses; the fruits are used in agriculture, are important as a food resource, and can be consumed in natura or processed. Due to a reduction in the area of ecosystems where it occurs, mangabeira is threatened by genetic erosio.. Read More»
Genet. Mol. Res. 16(3): gmr16039724
DOI: 10.4238/gmr16039724
Investigating genetic diversity in sapucaia using inter simple sequence repeat markers
Sapucaia is a tree species originating from the Brazilian Amazon and is widely distributed in Brazil, especially in the mid-north region (Piauí and Maranhão states). Its seeds are rich in calories and proteins, and possess great potential for commercialization. Little is known about the genetic variability in the germplasm of most Lecythis species. Here, 11 inter-simple sequence r.. Read More»
Genet. Mol. Res. 15(3): gmr.15038565
DOI: 10.4238/gmr.15038565
RAPD analysis of the genetic diversity of mango (Mangifera indica) germplasm in Brazil
We evaluated genetic variability of mango (Mangifera indica) accessions maintained in the Active Germplasm Bank of Embrapa Meio-Norte in Teresina, Piauí, Brazil, using RAPDs. Among these accessions, 35 originated from plantings in Brazil, six from the USA and one from India. Genomic DNA, extracted from leaf material using a commercial purification kit, was su.. Read More»
Genet. Mol. Res. 10(4): 2011.December.14.1
DOI: 10.4238/2011.December.14.1
DNase concentration assay to obtain DNA-free RNA from sugarcane leaves
The success of gene expression studies, protein synthesis, and construction of cDNA libraries directly depends on the purity and integrity of the RNA used in these tests, as even minor amounts of contaminant DNA (<1%) can produce a false positive amplification signal in quantitative real-time PCR. For RNA contaminated with genomic DNA, an essential step in the st.. Read More»
Genet. Mol. Res. 15(4): gmr15049073
DOI: 10.4238/gmr15049073
Chloroplast diversity of Casearia grandiflora in the Cerrado of Piauí State
Casearia grandiflora (Salicaceae) is a typical Cerrado species adapted to disturbed environments, making it useful for restoration projects. Knowledge of genetic diversity is important for establishing conservation strategies for this species. This study aimed to compare chloroplast haplotype diversity and structure of C. grandiflora, under the assumption that prote.. Read More»
Genet. Mol. Res. 16(1): gmr16019572
DOI: 10.4238/gmr16019572
Comparison of eight methods of genomic DNA extraction from babassu
Babassu (Orbignya phalerata Martius) is one of the most important palms in Brazil because of the largest morphological variation, wide geographic distribution, and high socio-economic importance. The diversity present in babassu germplasm should be protected against loss to ensure their use with high productivity. Study of the available variability in populations of babassu is necessary to deve.. Read More»
Genet. Mol. Res. 14(4): 2015.December.22.26
DOI: 10.4238/2015.December.22.26
Comparison of methods to isolate DNA from Caesalpinia ferrea
Molecular markers are important for characterizing the genetic diversity of plants and can provide the basis for strategies to protect and conserve endangered populations. However, numerous molecular techniques are used, requiring an evaluation of fast and efficient methods to extract DNA. Since molecular studies of Caesalpinia ferrea are rare, it is important to de.. Read More»
Genet. Mol. Res. 13(2): http://dx.doi.org/2014.June.16.7
DOI: http://dx.doi.org/10.4238/2014.June.16.7
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