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L.F. Marins

Publications of : L.F. Marins
Human Genetics   Research Article

Improving the PCR protocol to amplify a repetitive DNA sequence

Although PCR-based techniques have become an essential tool in the field of molecular and genetic research, the amplification of repetitive DNA sequences is limited. This is due to the truncated nature of the amplified sequences, which are also prone to errors during DNA polymerase-based amplification. The complex structure of repetitive DNA can form hairpin loops, which promote dissociation of.. Read More»

Genet. Mol. Res. 16(3): gmr16039796

DOI: 10.4238/gmr16039796

Bioinformatics   Research Article

Characterization of glucose-tolerant β-glucosidases used in biofuel production under the bioinformatics perspective: a systematic review

    D.C.B. Mariano, C. Leite, L.H.S. Santos, L.F. Marins, K.S. Machado, A.V. Werhli, L.H.F. Lima and R.C. de Melo-Minardi

β-glucosidases are enzymes that catalyze the hydrolysis of oligosaccharides and disaccharides, such as cellobiose. These enzymes play a key role in cellulose degrading, such as alleviating product inhibition of cellulases. Consequently, they have been considered essential for the biofuel industry. However, the majority of the characterized β-glucosidases i.. Read More»

Genet. Mol. Res. 16(3): gmr16039740

DOI: 10.4238/gmr16039740