J. Wang

Mac-1 deficiency induces respiratory failure by affecting type I alveolar epithelial cells

• J. Wang
• Y.B. Ci
• C.L. Liu
• H.M. Sun

As a β2 integrin family member, Mac-1 plays an important role in the inflammatory response. Inflammation and lung injury are closely associated, but the involvement of Mac-1 in the occurrence and development of such pathologies remains poorly understood. We aimed to investigate the relationship between Mac-1 deficiency and respiratory failure in Mac-1 knockout .. Read More»

Genet. Mol. Res. 16(3): gmr16039045

DOI: 10.4238/gmr16039045

Borax-induced apoptosis in HepG2 cells involves p53, Bcl-2, and Bax

Y. Wei1, F.J. Yuan, W.B. Zhou, L. Wu, L. Chen, J.J. Wang and Y.S. Zhang

Borax, a boron compound and a salt of boric acid, is known to inhibit the growth of tumor cells. HepG2 cells have been shown to be clearly susceptible to the anti-proliferative effects of borax. However, the specific mechanisms regulating this effect are poorly understood. This study aimed to investigate the pathways underlying the growth inhibition induced by borax.. Read More»

Genet. Mol. Res. 15(2):

Molecular cloning and characterization, and prokaryotic expression of the GnRH1 gene obtained from Jinghai yellow chicken

T. Zhang, G.X. Zhang, K.P. Han, Y. Tang, J.Y. Wang, Q.C. Fan, X.S. Chen, Y. Wei and Y.J. Wang

The gonadotropin-releasing hormone (GnRH) plays an important role in the control of reproductive functions. Recent studies have reported the occurrence of GnRH molecular variants in numerous species. In this study, the GnRH1 gene from Jinghai yellow chicken was cloned by reverse transcriptase-polymerase chain reaction and transformed into BL.. Read More»

Genet. Mol. Res. 14(1): http://dx.doi.org/2015.March.31.14

DOI: http://dx.doi.org/10.4238/2015.March.31.14

Apoptosis, proliferation, and morphology during vein graft remodeling in rabbits

L. Wan, S.H. Dai, S.Q. Lai, L.Q. Liu, Q. Wang, H. Xu, W.J. Wang and J.C. Liu

Neo-intima development and atherosclerosis limit the long-term use of vein grafts for revascularization of ischemic tissues. Recently, studies have confirmed that proliferating cell nuclear antigen (PCNA) plays an important role in cell proliferation. Our research confirmed that 28 days after vein transplantation, PCNA expression increases significantly. Using rabbi.. Read More»

Genet. Mol. Res. 15(4): gmr.15048701

DOI: 10.4238/gmr.15048701

Expression analysis of differentially expressed miRNAs in male and female chicken embryos

Y.P. Feng, J.F. Chen, P. Huang, X. Wang, J. Wang, X.L. Peng and Y.Z. Gong

MicroRNAs (miRNAs) are small non-coding RNA molecules that play key roles in the regulation of development processes of many tissues and organs at the post-transcriptional level. However, little is known about how they affect chicken gonadal development. We examined the expression of four miRNAs (miR-218, -200b, -196, and -206) in chicken embryonic gonads at embryon.. Read More»

Genet. Mol. Res. 13(2): 2014.April.17.2

DOI: 10.4238/2014.April.17.2

Association of PS1 1/2, ACE I/D, and LRP C/T polymorphisms with Alzheimer’s disease in the Chinese population: a meta-analysis of case-control studies

L. Yang, H.-H. Zhou, Y.-F. Ye, X.-W. Fan, Y.-J. Wang and Y. Meng

The objective of this study was to assess the associations of presenilin 1 (PS1) 1/2, angiotensin I-converting enzyme (ACE) insertion/deletion (I/D), and low-density lipoprotein receptor-related protein (LRP) C/T polymorphisms with the risk of Alzheimer’s disease (AD) in the Chinese population. PS1 1/2, ACE I/D, and LRP C/T, which are commonly investigated polymorphisms, were evaluated to.. Read More»

Genet. Mol. Res. 14(1): 2015.February.6.5

DOI: 10.4238/2015.February.6.5

Influence of the DCC gene on proliferation and carcinoembryonic antigen expression in the human colorectal cancer cell line SW1116

H.W. Jiang, J. Wang, H.J. Li, J.K. Peng, X.P. Gao and F. Chen

This study investigated the effects of stable transfection of the exogenous wild-type DCC gene on growth of the human colorectal carcinoma cell line SW1116 in vitro. The DCC gene was amplified from normal human colon tissue by reverse transcription-polymerase chain reaction and used to construct a recombinant expression plasmid, pcDNA3.1(+)-DCC. DCC-negative SW1116 cells were transfected with p.. Read More»

Genet. Mol. Res. 14(3): 2015.August.28.12

DOI: 10.4238/2015.August.28.12

Analysis of synonymous codon usage in FAD7 genes from different plant species

Q.P. Ma, C. Li, J. Wang, Y. Wang and Z.T. Ding

In this study, the codon bias of the FAD7 genes among 10 different plant species was analyzed to identify general patterns of codon usage in the FAD7 genes. Our results showed that U-ended or A-ended codons were preferentially used in FAD7 for dicots, whereas G-ended or C-ended codons were preferentially used in FAD7 for monocots. An ENC-plot showed that some other factors may influence the cod.. Read More»

Genet. Mol. Res. 14(1): 2015.February.13.20

DOI: 10.4238/2015.February.13.20

Role of glutathione and other antioxidants in the inhibition of apoptosis and mesenchymal transition in rabbit lens epithelial cells

H.J. Wang, J. Zhu and G.Y. Zheng

This study aimed to discuss the effect and mechanism of glutathione (GSH) and catalase (CAT) on transforming growth factor beta (TGF-β)-induced lens epithelial cell (LEC) apoptosis and epithelial mesenchymal transition (EMT) to prevent and control cataracts. Healthy rabbits (4 weeks old) were randomly selected, and LECs from their lenses were cultured in vitro... Read More»

Genet. Mol. Res. 13(3): 2014.September.1.1

DOI: 10.4238/2014.September.1.1

Comparison of EGFR mutation rates in lung adenocarcinoma tissue and pleural effusion samples

Y. Guan, Z.J. Wang, L.Q. Wang, D.F. Hua and J. Liu

The goal of the current study was to investigate the differences in epidermal growth factor receptor (EGFR) mutation rates in tumor tissue and pleural effusion specimens from patients with lung adenocarcinoma. PCR amplification and gene sequencing were used to detect EGFR mutations in exons 18, 19, 20, and 21 in tumor tissue and pleural effusion samples from 50 pati.. Read More»

Genet. Mol. Res. 15(2): gmr.15027001

DOI: 10.4238/gmr.15027001