A.M. Waldschmidt
An optimized protocol for DNA extraction in plants with a high content of secondary metabolites, based on leaves of Mimosa tenuiflora (Willd.) Poir. (Leguminosae)
Some species are characterized by a high content of tannins, alkaloids, and phenols in their leaves. These secondary metabolites are released during DNA extraction and might hinder molecular studies based on PCR (polymerase chain reaction). To provide an efficient method to extract DNA, Mimosa tenuiflora, an important leguminous plant from Brazilian semiarid region .. Read More»
Genet. Mol. Res. 16(3): gmr16039063
DOI: 10.4238/gmr16039063
Genetic variability in Melipona scutellaris from Recôncavo, Bahia, Brazil
Bees play a key role in pollination and thereby help maintain plant diversity. The stingless bee Melipona scutellaris is an important pollinator in northeastern Brazil because it is endemic to this region. Both deforestation and timber harvesting have reduced the nesting sites for this species, thus reducing its population and range. Genetic studies may help reverse this process by providing im.. Read More»
Genet. Mol. Res. 12(3): 2013.September.10.1
DOI: 10.4238/2013.September.10.1
Transferability of microsatellite primers developed for stingless bees to four other species of the genus Melipona
Microsatellite markers are a useful tool for ecological monitoring of natural and managed populations. A technical limitation is the necessity for investment in the development of primers. Heterologous primers can provide an alternative to searching for new loci. In bees, these markers have been used in populational and intracolonial genetic analyses. The genus Meli.. Read More»
Genet. Mol. Res. 10(4): 2011.November.22.11
DOI: 10.4238/2011.November.22.11
Optimization of DNA extraction from fresh leaf tissues of Melanoxylon brauna (Fabaceae)
Melanoxylon brauna (Fabaceae - Caesalpinioideae) is an endemic and valuable hardwood tree species in the Brazilian Atlantic rainforest; it is comparable to African ebony wood. We tested three protocols of DNA extraction based on the citrimonium bromide (CTAB) method and evaluated the quantity, purity and integrity of the DNA. We also determined whether these procedu.. Read More»
Genet. Mol. Res. 11(2): 2012.May.22.8
DOI: 10.4238/2012.May.22.8
Population structure and genetic diversity in Gynaikothrips uzeli (Thysanoptera: Phlaeothripidae): is there a correlation between genetic and geographic proximity?
Gynaikothrips uzeli (Thysanoptera: Phlaeothripidae) is a minuscule insect species, which forms galls, is subsocial, and parthenogenetic. It is associated with Ficus benjamina L. (Moraceae) and has a pantropical occurrence. The paucity of genetic studies on the order Thysanoptera led us to use inter-simple sequence repeat molecular marker to assess intra- and inter-gall, as well as intra- and in.. Read More»
Genet. Mol. Res. 14(3): 2015.August.19.12
DOI: 10.4238/2015.August.19.12
Transferability of microsatellite markers in Syagrus coronata (Mart.) Becc. (Arecaceae), an iconic palm tree from the Brazilian semiarid region
The licuri palm Syagrus coronata plays a key role in the ecology and economy of Brazilian semiarid region. Nonetheless, genetic data about populations of this species are absent even though the intensive and uncontrolled exploitation since colonial periods has threatened the sustainability and viability of licuri populations. Therefore, we attempted to test the effi.. Read More»
Genet. Mol. Res. 16(2): gmr16029699
DOI: 10.4238/gmr16029699
Genetic diversity and structure in natural populations of Maytenus truncata Reiss, 1861, a medicinal plant vulnerable to extractivism in Bahia State, Brazil
Maytenus truncata (Celastraceae) is a plant species widely used in the treatment of ulcers and tumors. Despite the intensive harvest of native specimens in the State of Bahia, northeastern Brazil, there is no information about the genetic variability or structure of this species. Therefore, the goal of this study was to estimate the genetic diversity and population structure of M. truncata base.. Read More»
Genet. Mol. Res. 14(4): 2015.December.23.11
DOI: 10.4238/2015.December.23.11
Occurrence of multiple nucleolus organizer regions and intraspecific karyotype variation in Scaptotrigona xanthotricha Moure (Hymenoptera, Meliponini)
Scaptotrigona xanthotricha has a wide geographic distribution in the Brazilian Atlantic rainforest. One population from southeast and two from northeast Brazil were analyzed and were found to have chromosome polymorphisms. Although the chromosome number 2n = 34 is conserved in this species, karyotypic analysis revealed clear differences between the three populations. Congruent an.. Read More»
Genet. Mol. Res. 8(3): vol8-3gmr598
DOI: 10.4238/vol8-3gmr598
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