Plant Genetics
Comparison of methods to isolate DNA from Caesalpinia ferrea
Authors: C.C. Sousa, S.O. Gomes, A.C.A. Lopes, R.L.F. Gomes, F.B. Britto, P.S.C. Lima and S.E.S. Valente
Molecular markers are important for characterizing the genetic diversity of plants and can provide the basis for strategies to protect and conserve endangered populations. However, numerous molecular techniques are used, requiring an evaluation of fast and efficient methods to extract DNA. Since molecular studies of Caesalpini.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.16.7
- DOI:
- http://dx.doi.org/10.4238/2014.June.16.7
Aluminum triggers broad changes in microRNA expression in rice roots
Authors: J.C. Lima, R.A. Arenhart, M. Margis-Pinheiro and R. Margis
MicroRNAs are small 21-nucleotide RNA molecules with regulatory roles in development and in response to stress. Expression of some plant miRNAs has been specifically associated with responses to abiotic stresses caused by cold, light, iron, and copper ions. In acid soils, aluminum solubility increases, thereby causing severe d.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.10.4
- DOI:
- 10.4238/2011.November.10.4
GmPTF1, a novel transcription factor gene,is involved in conferring soybean tolerance to phosphate starvation
Authors: X.H. Li, B. Wu, Y.B. Kong and C.Y. Zhang
Phosphorus plays a pivotal role in plant growth and development. In this study, we isolated and characterized GmPTF1, a basic helix-loop-helix (bHLH) transcription factor (TF) gene from soybean (Glycine max) with tolerance to inorganic phosphate (Pi) starvation. Alignment analysis indicated that GmPTF1 and other reported bHLH .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.19.3
- DOI:
- 10.4238/2014.February.19.3
Adaptability and stability of genotypes of sweet sorghum by GGEBiplot and Toler methods
Authors: U.J. de Figueiredo, J.A.R. Nunes, R.A. da C. Parrella, E.D. Souza, A.R. da Silva, B.M. Emygdio, J.R.A. Machado and F.D. Tardin
Sweet sorghum has considerable potential for ethanol and energy production. The crop is adaptable and can be grown under a wide range of cultivation conditions in marginal areas; however, studies of phenotypic stability are lacking under tropical conditions. Various methods can be used to assess the stability of the crop. Some of these methods generate th.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.22.15
- DOI:
- http://dx.doi.org/10.4238/2015.September.22.15
Generation of induced pluripotent mouse stem cells in an indirect co-culture system
Authors: F.L. Dong H.A. Kaleri Y.D. Lu C.L. Song B.C. Jiang B.L. Zhang L.J. Wang X.G. Wang X.S. Ma B.J. Wu H. Song J. Li H.L. Liu
Typically, production of induced pluripotent stem cells requires direct contact with feeder cells. However, once the stem cells have reached the appropriate maturation point, it is difficult to separate them from feeder cells, which must be irradiated with γ-rays or treated with the antibiotic mitomycin-C. We used a microporous poly-membrane-based i.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.December.6.1
- DOI:
- 10.4238/2012.December.6.1
Morphoagronomic and molecular profiling of Capsicum spp from southwest Mato Grosso, Brazil
Authors: A.L. Campos, T.N. Marostega, N.S.S. Cabral, K.L. Ara�ºjo, M.E. Serafim, S. Seabra-J�ºnior, C.P. Sudr�©, R. Rodrigues and L.G. Neves
The genus Capsicum ranks as the second most exported vegetable in Brazil, which is also considered to be a center of diversity for this genus. The aim of this study was to rescue genetic variability in the genus Capsicum in the southwest region of Mato Grosso, and to characterize and estimate the genetic diversity of accessions based on morphoagronomic de.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038167
- DOI:
- 10.4238/gmr.15038167
In-depth analysis of internal control genes for quantitative real-time PCR in Brassica oleracea var. botrytis
Authors: X.G. Sheng, Z.Q. Zhao, H.F. Yu, J.S. Wang, C.F. Zheng and H.H. Gu
Quantitative reverse-transcription PCR (qRT-PCR) is a versatile technique for the analysis of gene expression. The selection of stable reference genes is essential for the application of this technique. Cauliflower (Brassica oleracea L. var. botrytis) is a commonly consumed vegetable that is rich in vitamin, calcium, and iron. Thus far, to our knowledge, .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038348
- DOI:
- 10.4238/gmr.15038348
Development of polymorphic microsatellite markers based on expressed sequence tags in Populus cathayana (Salicaceae)
Authors: Z.Z. Tian F.Q. Zhang, Z.Y. Cai and S.L. Chen
Populus cathayana occupies a large area within the northern, central, and southwestern regions of China, and is considered to be an important reforestation species in western China. In order to investigate the population genetic structure of this species, 10 polymorphic microsatellite loci were identified based on expressed sequence tags from de novo sequ.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038406
- DOI:
- 10.4238/gmr.15038406
Evaluation of bone matrix gelatin/fibrin glue and chitosan/gelatin composite scaffolds for cartilage tissue engineering
Authors: Z.H. Wang, J. Zhang, Q. Zhang, Y. Gao, J. Yan, X.Y. Zhao, Y.Y. Yang, D.M. Kong, J. Zhao, Y.X. Shi and X.L. Li
This study was designed to evaluate bone matrix gelatin (BMG)/fibrin glue and chitosan/gelatin composite scaffolds for cartilage tissue engineering. Chondrocytes were isolated from costal cartilage of Sprague-Dawley rats and seeded on BMG/fibrin glue or chitosan/gelatin composite scaffolds. After different in vitro culture durations, the scaffolds were su.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038431
- DOI:
- 10.4238/gmr.15038431
Development of expressed sequence tag-based microsatellite markers for the critically endangered Iso�«tes sinensis (Isoetaceae) based on transcriptome analysis
Authors: A.W. Gichira, Z.C. Long, Q.F. Wang, J.M. Chen and K. Liao
Isoëtes sinensis is a critically endangered quillwort. To facilitate studies on the conservation genetics of this species, we developed expressed sequence tag-simple sequence repeat (EST-SSR) markers. A total of 50,063 unigenes were predicted by transcriptome sequencing, 5294 (10.6%) of which significantly matched 3011 Gene Ontology annotations and 2.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038497
- DOI:
- 10.4238/gmr.15038497