Plant Genetics

Establishing references for gene expression analyses by RT-qPCR in Theobroma cacao tissues

Authors: T.T. Pinheiro, C.G. Litholdo Jr., M.L. Sereno, G.A. Leal Jr., P.S.B. Albuquerque and A. Figueira

Lack of continuous progress in Theobroma cacao (Malvaceae) breeding, especially associated with seed quality traits, requires more efficient selection methods based on genomic information. Reverse transcript quantitative PCR (RT-qPCR) has become the method of choice for gene expression analysis, but relative expression analysi.. Read More»

Genet. Mol. Res. 10(4):

2011.November.17.4

DOI:

10.4238/2011.November.17.4

Pattern of CsICE1 expression under cold or drought treatment and functional verification through analysis of transgenic Arabidopsis

Authors: Z.T. Ding, C. Li, H. Shi, H. Wang and Y. Wang

CsICE1 is thought to be involved in hardiness resistance of tea plants. Using seedling cuttings of biennial Wuniuzao in this study, the pattern of CsICE1 expression under cold temperature (4°, -5°C), drought [20% polyethylene glycol 6000 (PEG-6000)], and plant hormone [200 mg/L abscisic acid (ABA), 1 mg/L brassinolide (BR)] treatment was studied b.. Read More»

Genet. Mol. Res. 14(3):

http://dx.doi.org/2015.September.22.20

DOI:

http://dx.doi.org/10.4238/2015.September.22.20

Karyotype characterization of two populations of Vernonia geminata (Asteraceae, Vernonieae) using banding and FISH techniques

Authors: V.M. Oliveira M.E. Mansanares J. Semir E.R. Forni-Martins

In order to extend our knowledge concerning karyotypes of the genus Vernonia, we applied various techniques of chromosome banding, including AgNOR and triple staining with the fluorochromes CMA/DA/DAPI (CDD), and of fluorescent in situ hybridization (FISH) for the 45S rDNA probe to specimens of two populations of Vernonia geminata collected from an open-p.. Read More»

Genet. Mol. Res. 11(4):

2012.September.25.1

DOI:

10.4238/2012.September.25.1

Science, Evolution and Creationism

Authors: D. De Jong

Genet. Mol. Res. 7(4):

vol7-4gmr565

DOI:

10.4238/vol7-4gmr565

Estimation of genetic diversity among sunflower genotypes through random amplified polymorphic DNA analysis

Authors: M.A. Iqbal, H.A. Sadaqat and I.A. Khan

The genetic diversity among eight sunflower lines was determined through the estimation of the random amplified polymorphic DNA method. One hundred and fifty-six DNA fragments were generated by 20 random primers, for an average of about 7.8 bands per primer. Of these amplified DNA fragments, 104 were polymorphic among the eight sunflower lines. Nei and Li.. Read More»

Genet. Mol. Res. 7(4):

vol7-4gmr494

DOI:

10.4238/vol7-4gmr494

Application of 16S rRNA, cytochrome b and control region sequences for understanding the phylogenetic relationships in Oryx species

Authors: H.A. Khan, I.A. Arif, A.A. Al Homaidan and A.H. Al Farhan

The present study reports the application of mitochondrial markers for the molecular phylogeny of Oryx species, including the Arabian oryx (AO), scimitar-horned oryx (SHO) and plains oryx (PO), using the Addax as an outgroup. Sequences of three molecular markers, 16S rRNA, cytochrome b and a control region, for the above four taxa were aligned and the top.. Read More»

Genet. Mol. Res. 7(4):

vol7-4gmr514

DOI:

10.4238/vol7-4gmr514

Activity and expression of polygalacturonase vary at different fruit ripening stages of sweet pepper cultivars

Authors: S.S. Ahmed, Z.-H. Gong, M.A. Khan, Y.-X. Yin, W.-L. Guo and J. Imran

Activity and expression of polygalacturonase (PG), a hydrolytic enzyme involved in ultrastructural changes in the pericarp of sweet pepper (Capsicum annaum), were investigated at different ripening stages of the pepper cultivars Mandi and Talanduo. Molecular cloning of CaPG was carried out by constructing a cDNA library from t.. Read More»

Genet. Mol. Res. 10(4):

2011.November.22.10

DOI:

10.4238/2011.November.22.10

Heteropicnotic chromatin and nucleolar activity in meiosis and spermiogenesis of Limnogonus aduncus (Heteroptera, Gerridae): a stained nucleolar organizing region that can serve as a model for studying chromosome behavior

Authors: M.M.U. Castanhole, L.L.V. Pereira, H.V. Souza, H.E.M.C. Bicudo, L.A.A. Costa and M.M. Itoyama

Males of Limnogonus aduncus were found to have the sex chromosome system X0 and chromosome number 2n = 23 (22A + X0). Testis cells were stained with lacto-acetic orcein and silver nitrate so that changes in the morphology and degree of staining of the heteropicnotic chromatin and the nucleolar material could be observed during meiosis and spermiogenesis. .. Read More»

Genet. Mol. Res. 7(4):

vol7-4gmr527

DOI:

10.4238/vol7-4gmr527

Evaluation of the genotoxic activity of ethanol extract and secondary metabolites isolated from Aiouea trinervis Meisn. (Lauraceae)..

Authors: Z.R. Guterres, F.R. Garcez, W.S. Garcez, L.M.G.E. Silva, A.F.G. Silva, C.U.N.B.D. Duarte and V.F. Batista-Silva

Aiouea trinervis Meisn. is a shrub that grows in the “Cerrado” (a savanna ecosystem) of Brazil. In this study, fractionation of ethanol extracts (EEs) from the leaves of A. trinervis led to the isolation of butanolides, namely isoobtusilactone A and obtusilactone A, as well as lignans, namely sesamin, methylpiperit.. Read More»

Genet. Mol. Res. 13(1):

http://dx.doi.org/2014.February.19.8

DOI:

http://dx.doi.org/10.4238/2014.February.19.8

Cloning and expression pattern analysis of BkGRAS2 from Betula kirghisorum

Authors: G.Y. Li, C.J. Yang and G.J. Liu

GRAS proteins are plant-specific transcription factors that are involved in the regulation of root and shoot growth. Here, we cloned BkGRAS2 from Betula kirghisorum (abbreviated to Bk) and analyzed the physicochemical properties and expression pattern of the encoded protein. BkGRAS2 had an open reading frame of 1614 bp encoding 537 amino acid residues. Th.. Read More»

Genet. Mol. Res. 14(3):

http://dx.doi.org/2015.September.22.27

DOI:

http://dx.doi.org/10.4238/2015.September.22.27