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Plant Genetics

Plant Genetics   Research Article

Expression patterns of Doppel in differential ovine PRNP genotypes: quantification using real-time RT-PCR

Authors: C. Wang, C.-L. Zhao, L. Liu, R. Wu and X.-L. Zhang

Doppel is a homologue of cellular prion protein (PrP)-like protein (PrPC). Different tissue samples were collected from the central nervous system plus four regions of lymphoid system, eleven regions of digestive tract and two reproductive organs from four ARR/ARQ and four ARH/ARQ sheep, genotypes of the PrP gene. Total RNA wa.. Read More»

Genet. Mol. Res. 14(4):
2015.October.9.3
DOI:
10.4238/2015.October.9.3
Plant Genetics   Research Article

Phylogenetic relationship of Turkish Apis mellifera subspecies based on sequencing of mitochondrial cytochrome C oxidase I region

Authors: F. ��zdil and F. �°lhan

Mitochondrial DNA sequence variation can be used to infer honey bee evolutionary relationships. We examined DNA sequence diversity in the cytochrome C oxidase I (COI or Cox1) gene segment of the mitochondrial genome in 112 samples of Apis mellifera from 15 different populations in Turkey. Six novel haplotypes were found for th.. Read More»

Genet. Mol. Res. 11(2):
2012.April.27.12
DOI:
10.4238/2012.April.27.12
Plant Genetics   Research Article

Characterization and expression analysis of somatic embryogenesis receptor-like kinase genes from Phalaenopsis

Authors: Y.W. Huang, Y.J. Tsai and F.C. Chen

Somatic embryogenesis receptor-like kinase (SERK) genes have been found to be involved in the somatic embryogenesis of several plant species. We identified and characterized 5 PhSERK genes in the Phalaenopsis orchid. The amino acid sequences of PhSERKs and other SERK proteins are highly conserved, with the highest homology observed in the leucine-rich rep.. Read More»

Genet. Mol. Res. 13(4):
2014.December.18.11
DOI:
10.4238/2014.December.18.11
Plant Genetics   Research Article

Development of SNP markers and their application for genetic diversity analysis in the oil palm (Elaeis guineensis)

Authors: P.W. Ong, I. Maizura, N.A.P. Abdullah, M.Y. Rafii, L.C.L. Ooi, E.T.L. Low and R. Singh

The genetic evaluation of oil palm germplasm collections is required for insight into the variability among populations. The information obtained is also useful for incorporating new genetic materials into current breeding programs. Single nucleotide polymorphisms (SNPs) have been widely used in many plant genetic studies due .. Read More»

Genet. Mol. Res. 14(4):
2015.October.9.9
DOI:
10.4238/2015.October.9.9
Plant Genetics   Research Article

Event-specific qualitative and quantitative PCR detection of the GMO carnation (Dianthus caryophyllus) variety Moonlite based upon the 5'-transgene integration sequence

Authors: P. Li, J.W. Jia, L.X. Jiang, H. Zhu, L. Bai, J.B. Wang, X.M. Tang and A.H. Pan

To ensure the implementation of genetically modified organism (GMO)-labeling regulations, an event-specific detection method was developed based on the junction sequence of an exogenous integrant in the transgenic carnation variety Moonlite. The 5êÂ?Â?-transgene integration sequence was isolated by thermal asymmetric interlac.. Read More»

Genet. Mol. Res. 11(2):
2012.April.27.11
DOI:
10.4238/2012.April.27.11
Plant Genetics   Research Article

Gene expression profiling of somatic and pluripotent cells reveals novel pathways involved in reprogramming

Authors: Y.N. Cai, X.H. Dai, Q.H. Zhang, R.Hu and Z.M. Dai

We investigated gene expression in embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, and fibroblasts. Microarray expression data sets obtained from the Gene Expression Omnibus were analyzed using the Partek software. Human genes from ES cells, iPS cells, and fibroblasts were ranked from low to high according to .. Read More»

Genet. Mol. Res. 14(4):
2015.October.5.21
DOI:
10.4238/2015.October.5.21
Plant Genetics   Research Article

New microsatellite markers for bananas (Musa spp)

Authors: E.P. Amorim, P.H. Silva, C.F. Ferreira, V.B.O. Amorim, V.J. Santos, A.D. Vilarinhos, C.M.R. Santos, M.T. Souza J�ºnior and R.N.G. Miller

Thirty-four microsatellite markers (SSRs) were identified in EST and BAC clones from Musa acuminata burmannicoides var. Calcutta 4 and validated in 22 Musa genotypes from the Banana Germplasm Bank of Embrapa-CNPMF, which includes wild and improved diploids. The number of alleles per locus ranged from 2 to 14. The markers were .. Read More»

Genet. Mol. Res. 11(2):
2012.April.27.8
DOI:
10.4238/2012.April.27.8
Plant Genetics   Research Article

Genetic analyses, phenotypic adaptability and stability in sugarcane genotypes for commercial cultivation in Pernambuco

Authors: J.A. Dutra Filho; T.C. Junior and D.E. Sim�µes Neto

In the present study, we assessed the agro-industrial performance of 22 sugarcane genotypes adaptable to edaphoclimatic conditions in production microregions in the State of Pernambuco, Brazil, and we recommended the commercial cultivation of select genotypes. The variables analyzed were as follows: sucrose percentage in cane .. Read More»

Genet. Mol. Res. 14(4):
2015.October.5.23
DOI:
10.4238/2015.October.5.23
Plant Genetics   Research Article

Characterization and development of chloroplast microsatellite markers for Gossypium hirsutum, and cross-species amplification in other Gossypium species

Authors: X.Y. Cai, F. Liu, Z.L. Zhou, X.X. Wang, C.Y. Wang, Y.H. Wang and K.B. Wang

Cotton is an important economic crop worldwide; its fiber, commonly known as cotton lint, is the main natural source for the textile industry. Sixty chloroplast microsatellites were identified and characterized from the complete sequence of the Gossypium hirsutum chloroplast genome using a bioinformatic approach. Twenty chloro.. Read More»

Genet. Mol. Res. 14(4):
2015.October.5.6
DOI:
10.4238/2015.October.5.6
Plant Genetics   Research Article

Arylamine N-acetyltransferase 2 genotypes in a Mexican population..

Authors: L. Taja-Chayeb, J.A. Ag�ºndez, C. Miguez-Mu�±oz, A. Chavez-Blanco and A. Due�±as-Gonzalez

The acetylating activity of N-acetyltransferase 2 (NAT2) has critical implications for therapeutics and disease susceptibility. To date, several polymorphisms that alter the enzymatic activity and/or protein stability of NAT2 have been identified. We examined the distribution and frequency of NAT2 genotypes in the Mexican popu.. Read More»

Genet. Mol. Res. 11(2):
2012.April.27.7
DOI:
10.4238/2012.April.27.7