Plant Genetics
Novel polymorphic microsatellite markers isolated from the pen shell Atrina pectinata (Mollusca: Bivalvia: Pinnidae)
Authors: B. Wu, L.J. Bai, A.G. Yang, L.Q. Zhou and Z.H. Liu
In this study, we isolated 21 novel polymorphic microsatellite DNA loci from the pen shell Atrina pectinata using magnetic-bead hybridization enrichment. The characteristics of these loci were tested using a population of 30 individuals collected from the Penglai coast, Shandong Province. The number of alleles ranged from 2 to 13, and polymorphism informa.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.18.6
- DOI:
- 10.4238/2014.December.18.6
DNA isolation protocol for the medicinal plant lemon balm (Melissa officinalis, Lamiaceae)
Authors: S. Ghaffariyan, S.A. Mohammadi and S. Aharizad
Lemon balm (Melissa officinalis) is a medicinal plant that is widely used as a sedative or calmant, spasmolytic and antibacterial agent and sleep aid. This has led to a high demand for lemon balm products, resulting in the extinction of this species in some of its natural habitats. Molecular techniques have increasingly been u.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.27.3
- DOI:
- 10.4238/2012.April.27.3
Single- and double-SSR primer combined analyses in rice
Authors: J. Ma, S.C. Guan, Z. Zhang and P.W. Wang
Polymerase chain reaction (PCR) is the foundation of SSR molecular marker technology. We used sib rice varieties J518, XD1 and SD23 as experimental materials, selecting 30 pairs of SSR primers, including RM127, RM337 and RM5172, covering the rice genome, and performed single- and double-SSR primer combined analyses. We found t.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.27.1
- DOI:
- 10.4238/2012.April.27.1
An AFLP-based approach for the identification of sex-linked markers in blunt snout bream, Megalobrama amblycephala (Cyprinidae)
Authors: H.O. Rao, J.C. Deng, W.M. Wang and Z.-X. Gao
Sex-specific DNA markers are useful for studying sex-determination mechanisms and establishment of monosex populations. Three widely spaced geographical populations (Liangzi, Poyang and Yuni Lakes in China) of blunt snout bream (Megalobrama amblycephala) were screened with AFLPs to search for sex-linked markers. Female and mal.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.19.7
- DOI:
- 10.4238/2012.April.19.7
Identification of RNA editing sites in cotton (Gossypium hirsutum) chloroplasts and editing events that affect secondary and three-dimensional protein structures
Authors: Y. Jiang, S.L. Fan, M.Z. Song, J.N. Yu and S.X. Yu
RNA editing can alter individual nucleotides in primary transcripts, which can cause the amino acids encoded by edited RNA to deviate from the ones predicted from the DNA template. We investigated RNA editing sites of protein-coding genes from the chloroplast genome of cotton. Fifty-four editing sites were identified in 27 tra.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.19.4
- DOI:
- 10.4238/2012.April.19.4
Cloning flanking sequence by single-primer PCR in transgenic plants
Authors: J. Ma1, Y.P. Wang1, S. Ren2, Z. Zhang1, S. Lu1 and P.W. Wang1
The insertion position of exogenous genes in plant genomes is usually identified by adapter ligation-mediated polymerase chain reaction (PCR), thermal asymmetric interlaced PCR, and restriction site extension PCR in transgenic plant research. However, these methods have various limitations, such as the complexity of designing primers and time-consuming an.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.October.20.16
- DOI:
- 10.4238/2014.October.20.16
Molecular cloning and characterization of a proliferating cell nuclear antigen gene by chemically induced male sterility in wheat (Triticum aestivum L.)
Authors: Y.A. Yu, G.S. Zhang, J. Zhang, L. Ju, Q.D. Zhu, Y.L. Song, J.W. Wang, N. Niu and S.C. Ma
Although a number of studies have shown that chemical hybridizing agents (CHAs) affect anther growth and regulate cell-cycle progression, little is known about the molecular and cellular mechanisms involved. Proliferating cell nuclear antigen (PCNA) is an essential factor in DNA replication, and in many other processes in euka.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.16
- DOI:
- 10.4238/2015.October.5.16
Identification and bioinformatic analysis of a putative calcium-dependent protein kinase (CDPK6) from Toxoplasma gondii
Authors: N.Z. Zhang, S.Y. Huang, D.H. Zhou, Y. Xu, J.J. He and X.Q. Zhu
Toxoplasma gondii is recognized as an opportunistic human pathogen with a worldwide distribution. Development of effective vaccines is considered the only ideal way to control T. gondii infection. However, only one live vaccine is commercially available for use in sheep and goats. Therefore, the identification of more effective antigenic proteins is very .. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.18.9
- DOI:
- 10.4238/2014.December.18.9
An improved strategy based on RAPD markers efficiently identified 95 peach cultivars
Authors: M.L. Yu, W.Y. Wang, R.J. Ma, Z.J. Shen and J.G. Fang
DNA markers have useful applications in cultivar identification. A novel analysis approach called cultivar identification diagram (CID) was developed using DNA markers in the separation of plant individuals. This new strategy is less time- and cost-consuming, has reliable results, and was constructed for fingerprinting. Ten 11.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.7.1
- DOI:
- 10.4238/2012.May.7.1
Molecular cloning and expression analysis of five GhRAXs in upland cotton (Gossypium hirsutum L.)
Authors: T.C. Dai and Z.M. Wang
The formation of axillary meristems in leaf axils is a prerequisite for the development of lateral shoots, which largely contribute to plant architecture. Several transcription factor-encoding genes, including CUC3, RAX, LAS, LOF1, and ROX, have been cloned by screening for axillary meristem mutants in Arabidopsis thaliana. Th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.25
- DOI:
- 10.4238/2015.October.5.25