Plant Genetics
Acanthopanax senticosus attenuates inflammation in lipopolysaccharide-induced acute lung injury by inhibiting the NF-�ºB pathway
Authors: X.J. Fei, L.L. Zhu, L.M. Xia, W.B. Peng and Q. Wang
The dried roots of the plant Acanthopanax senticosus (AS) are used in traditional Oriental medicine and reportedly possess anti-inflammatory properties in vitro. However, whether AS has the same anti-inflammatory effect in vivo and the underlying mechanisms of this action remain unknown. In this study, we pretreated a mouse model of lipopolysaccharide-ind.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.12.16
- DOI:
- 10.4238/2014.December.12.16
Genetic divergence of physiological-quality traits of seeds in a population of peppers
Authors: A.M.S. Pessoa, P.A. Barroso, E.R. do R�ªgo, G.D.A. Medeiros, R.L.A. Bruno and M.M. do R�ªgo
Brazil has a great diversity of Capsicum peppers that can be used in breeding programs. The objective of this study was to evaluate genetic variation in traits related to the physiological quality of seeds of Capsicum annuum L. in a segregating F2 population and its parents. A total of 250 seeds produced by selfing in the F1 g.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.16.15
- DOI:
- 10.4238/2015.October.16.15
Analysis of gene expression patterns and levels in maize hybrids and their parents
Authors: H.S. Nie, S.P. Li, X.H. Shan, Y. Wu, S.Z. Su, H.K. Liu, J.Y. Han and Y.P. Yuan
Heterosis has greatly contributed to conventional plant breeding and is widely used to increase crop plant productivity. However, although some studies have explored the mechanisms of heterosis at the genomic and transcriptome level, these mechanisms still remain unclear. The growth and development of maize seedlings and immat.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.30.17
- DOI:
- 10.4238/2015.November.30.17
Evaluation of genetic divergence among clones of conilon coffee after scheduled cycle pruning
Authors: J.M. Dalcomo, H.D. Vieira, A. Ferreira, W.L. Lima, R.G. Ferr�£o, A.F.A. Fonseca, M.A.G. Ferr�£o and F.L. Partelli
Coffea canephora genotypes from the breeding program of Instituto Capixaba de Pesquisa e Extensão Rural were evaluated, and genetic diversity was estimated with the aim of future improvement strategies. From an initial group of 55 genotypes, 18 from the region of Castelo, ES, were selected, and three clones of .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.30.19
- DOI:
- 10.4238/2015.November.30.19
LC-MS/MS: a rapid and simple new method for the determination of carbapenem �²-lactamases
Authors: J.L. Shen, Y.H. Xu and T.T. She
We investigated the application of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for pan-drug-resistant Acinetobacter baumannii (PDR-AB) with carbapenem resistance. Eight strains were randomly selected from 84 clinical isolates of PDR-AB strains obtained by the Kirby-Bauer and agar dilution methods. An efflux pump inhibition test was used to s.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.8
- DOI:
- 10.4238/2015.November.18.8
Cloning of flanking sequence in transgenic plants by restriction site-anchored single-primer polymerase chain reaction
Authors: J. Ma, N.N. Wang, S. Ren, Y.P. Fu, S. Lu, Y.P. Wang and P.W. Wang
Determining the insertion position of an exogenous gene in the target plant genome is one of the main issues in the transgenic plant field. This study introduced a simple, rapid, and accurate method to clone the flanking sequences of the transgenic bar gene as the anchoring gene in the transgenic maize genome using single-primer polymerase chain reaction .. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.12.18
- DOI:
- 10.4238/2014.December.12.18
Proteome analysis of tobacco leaves reveals dynamic changes in protein expression among different cultivation areas
Authors: L.M. Lu, K.Y. Ye, Z.X. Tang, L. Liu, Y. Chen, J. Luo and Y.B. Huang
The leaves of tobacco plants were used to analyze differences in protein content of tobacco grown in the four main flue-cured tobacco-producing areas of Sichuan Province, China. An improved protein extraction method, isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis two-dimensional gel electrophore.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.30.3
- DOI:
- 10.4238/2015.November.30.3
Protein expression and characterization of SEP3 from Arabidopsis thaliana
Authors: Q. Shi, J. Zhou, P. Wang, X. Lin and Y. Xu
SEPALLATA (SEP) MADS-box genes play crucial roles in the regulation of floral growth and development. They are required for the specification of sepals, petals, stamens, and carpels as well as for floral determinacy. SEPs perform their functions through the formation of homo- or hetero-polymers, which are the molecular basis o.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.16.20
- DOI:
- 10.4238/2015.October.16.20
Cloning and characterization of a benzoic acid/salicylic acid carboxyl methyltransferase gene involved in floral scent production from lily (Lilium �Yelloween�)
Authors: H. Wang, M. Sun, L.L. Li, X.H. Xie and Q.X. Zhang
In lily flowers, the volatile ester methyl benzoate is one of the major and abundant floral scent compounds; however, knowledge regarding the biosynthesis of methyl benzoate remains unknown for Lilium. In this study, we isolated a benzoic acid/salicylic acid carboxyl methyltransferase (BSMT) gene, LiBSMT, from petals of Lilium ‘Yelloween’. The.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.14
- DOI:
- 10.4238/2015.November.18.14
Molecular characterization of aflatoxigenic and non-aflatoxigenic Aspergillus flavus isolates collected from corn grains
Authors: M.A. Mahmoud1, H.M. Ali2, A.R.M. El-Aziz2, M.R. Al-Othman2 and A.S. Al-Wadai2
Twelve species from six fungal genera were found to be associated with corn (Zea mays L.) grain samples collected from three main regions of Saudi Arabia. The average frequencies of the most common genera were Aspergillus (11.4%), Fusarium (9.5%), Penicillium (5.1%), and Alternaria (5.8%). Fifteen isolates of Aspergillus flavus were screened by HPLC for t.. Read More»
- Genet. Mol. Res. 13(4):