Plant Genetics
Genetic diversity analysis of Jatropha curcas L. (Euphorbiaceae) based on methylation-sensitive amplification polymorphism
Authors: T. Kanchanaketu, N. Sangduen, T. Toojinda and V. Hongtrakul
Genetic analysis of 56 samples of Jatropha curcas L. collected from Thailand and other countries was performed using the methylation-sensitive amplification polymorphism (MSAP) technique. Nine primer combinations were used to generate MSAP fingerprints. When the data were interpreted as amplified fragment length polymorphism (.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.13.2
- DOI:
- 10.4238/2012.April.13.2
Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain
Authors: F.J. Delgado-Martinez, I. Amaya, J.F. S�¡nchez-Sevilla and M.C. Gomez-Jimenez
Seventy-seven olive accessions corresponding to 25 cultivars from the Extremadura region of Spain were studied using four microsatellite or SSR markers in order to fingerprint them, and evaluate genetic similarity and relationships between local and introduced olive cultivars. The number of alleles per locus ranged from 4 to 8.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.10.7
- DOI:
- 10.4238/2012.April.10.7
Methylation-sensitive amplified polymorphism-based genome-wide analysis of cytosine methylation profiles in Nicotiana tabacum cultivars
Authors: J. Jiao, J. Wu, Z. Lv, C. Sun, L. Gao, X. Yan, L. Cui, Z. Tang, B. Yan and Y. Jia
This study aimed to investigate cytosine methylation profiles in different tobacco (Nicotiana tabacum) cultivars grown in China. Methylation-sensitive amplified polymorphism was used to analyze genome-wide global methylation profiles in four tobacco cultivars (Yunyan 85, NC89, K326, and Yunyan 87). Amplicons with meth.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.25.6
- DOI:
- 10.4238/2015.November.25.6
Genetic structure and diversity analysis of the primary gene pool of chickpea using SSR markers
Authors: P. Choudhary, S.M. Khanna, P.K. Jain, C. Bharadwaj, J. Kumar, P.C. Lakhera and R. Srinivasan
Members of the primary gene pool of the chickpea, including 38 accessions of Cicer arietinum, six of C. reticulatum and four of C. echinospermum grown in India were investigated using 100 SSR markers to analyze their genetic structure, diversity and relationships. We found considerable diversity, with a mean of 4.8 alleles per.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.10.5
- DOI:
- 10.4238/2012.April.10.5
Characterization of ESTs from black locust for gene discovery and marker development
Authors: J.X. Wang, C. Lu, C.Q. Yuan, B.B. Cui, Q.D. Qiu, P. Sun, R.Y. Hu, D.C. Wu, Y.H. Sun and Y. Li
Black locust (Robinia pseudoacacia L.) is an ecologically and economically important species. However, it has relatively underdeveloped genomic resources, and this limits gene discovery and marker-assisted selective breeding. In the present study, we obtained large-scale transcriptome data using a next-generation sequencing pl.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.19.12
- DOI:
- 10.4238/2015.October.19.12
Molecular characteristics and cloning of two pepper genes AN2 and UPA20
Authors: H.X. Chen, H. Jiang, J.L. Huo, J.F. Wen, H.S. Zhu, C.H. Ma, H. Zhou, J.H. Lv and M.H. Deng
The complete coding sequences (CDSs) of “Yunnan Purple Pepper No.1” (Capsicum annuum L.) AN2 and UPA20 genes were amplified using the reverse transcriptase polymerase chain reaction on the basis of the conserved sequence information of some Solanaceae plants and known highly homologous pepper expressed sequence tags. The nucleotide sequence an.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.January.17.6
- DOI:
- 10.4238/2014.January.17.6
Cloning and functional analysis of the chitinase gene promoter in peanut
Authors: X.L. Chen, R.T. Song, M.Y. Yu, J.M. Sui, J.S. Wang and L.X. Qiao
Chitinase is an important pathogenesis-related protein in plants, and it can accumulate when induced by salicylic acid (SA) or other elicitors. Here, we found that chitinase mRNA levels were 4.5-times greater when peanut seedlings were sprayed with 1.5 mM SA, as compared to water. The upstream promoter sequence of the chitinas.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.19.15
- DOI:
- 10.4238/2015.October.19.15
Homology-based analysis of the GRAS gene family in tobacco
Authors: Y.Q. Chen, S.S. Tai, D.W. Wang, A.M. Ding, T.T. Sun, W.F. Wang and Y.H. Sun
Members of the GRAS gene family are important transcriptional regulators. In this study, 21 GRAS genes were identified from tobacco, and were classified into eight subgroups according to the classification of Arabidopsis thaliana. Here, we provide a preliminary overview of this gene family in tobacco, describ.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.25.7
- DOI:
- 10.4238/2015.November.25.7
Effects of exogenous 5-aminolevulinic acid on PIP1 and NIP aquaporin gene expression in seedlings of cucumber cultivars subjected to salinity stress
Authors: F. Yan, D. Qu, Y.Y. Zhao, X.H. Hu, Z.Y. Zhao, Y. Zhang and Z.R. Zou
Aquaporins play a direct role in plant water relation under salt stress, but the effects of 5-aminolevulinic acid (ALA) on aquaporin gene expression in salt-treated plants remain unknown. This study investigated the potential effects of exogenous ALA (50 mg/dm3) on aquaporin expression levels under salt stress (75 mM NaCl) in .. Read More»
- Genet. Mol. Res. 13(2):
- 2014.January.22.12
- DOI:
- 10.4238/2014.January.22.12
Analysis of diversity and relationships among orchardgrass (Dactylis glomerata L.) accessions using start codon-targeted markers
Authors: L.F. Jiang, X. Qi, X.Q. Zhang, L.K. Huang, X. Ma and W.G. Xie
Orchardgrass, or cocksfoot, is an important perennial forage grass worldwide. The comprehensive understanding of orchardgrass accessions will benefit germplasm collection and breeding progress, and it will enhance efforts to improve forage yield and quality. Therefore, 24 novel, simple, polymorphic, and reliable start codon-ta.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.11.4
- DOI:
- http://dx.doi.org/10.4238/2014.June.11.4