Microbial Genetics
Interactome analysis and design of inhibitors against selected protein targets of Ser/Thr protein kinase (STPK) signaling pathways in Mycobacterium tuberculosis H37Rv
Authors: A.B. Gurung, B.J. Mylliemngap, A. Bhattacharjee1, M.A. Ali and F.M.A. Al-Hemaid
Tuberculosis continues to be a major cause of mortality worldwide despite significant advances in chemotherapy and development of the BCG vaccine. Although curable, the tuberculosis treatment period (6-9 months) presents many concerns, including patient noncompliance and the development of drug toxicity and drug resistance. This study aimed to understand .. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.1.6
- DOI:
- http://dx.doi.org/10.4238/2015.September.1.6
Identification of Glomerella cingulata f. sp phaseoli recombinants by RAPD markers
Authors: O.A. Camargo Junior, E.A. Souza, M.C. Mendes-Costa, J.B. Santos, M.A. Soares
We examined the capacity of strains of Glomerella cin-gulata f. sp phaseoli fungus (Colletotrichum lindemuthianum sexual stage) to form recombinants, using random amplified polymorphic DNA (RAPD). Crosses of all possible combinations between strains 40, 42, 20, 21, 22, 23, 24, 25, and 26 were made on Petri dishes using M3 cult.. Read More»
- Genet. Mol. Res. 6(3):
Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide
Authors: L.C.T. Thiesen, E.Y.Y. Sugauara, V. Te?¡evi??, J. Glamo?lija, M. Sokovi??, J.E. Gon?§alves, Z.C. Gazim, G.A. Linde and N.B. Colauto
Brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. However, studies on the antimicrobial activity of Brunfelsia uniflora flower oleoresin have not been found yet. This study aimed to evaluate the chemical composition and antimicrobial activity of.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029548
- DOI:
- 10.4238/gmr16029548
Vegetative compatibility and parasexual segregation in Colletotrichum lindemuthianum, a fungal pathogen of the common bean
Authors: M.A.A. Castro-Prado, C.B. Querol, J.R. Santâ??Anna, C.T. Miyamoto, C.C.S. Franco, C.A. Mangolin, M.F.P.S. Machado
The heterokaryotic and vegetative diploid phases of Colletotrichum lindemuthianum are described using nutritional and biochemical markers. Nitrate non-utilizing mutants (nit), derived from R2047, R89, R73, R65, and R23 isolates, were paired in all possible combinations to obtain heterokaryons. Although pairings R2047/R89, R204.. Read More»
- Genet. Mol. Res. 6(3):
A possible way that Ï?C31 integrase regulates the recombination direction
Authors: T.A. Liu, Y.X. Fang, H. Yin and Z.Z. Jing
φC31 integrase encoded by Streptomyces phage can mediate site-specific recombination between phage and host genomes. The recombination direction is generally considered to be accurately regulated, but the regulatory mechanisms involved are still unclear. Recently, some hyperactive mutants of φC31 integrase that can bypass the regulatory steps have.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.26.4
- DOI:
- 10.4238/2015.January.26.4
Apoptosis in different gastric lesions and gastric cancer: relationship with Helicobacter pylori, overexpression of p53 and aneuploidy
Authors: A.C. Targa, A.C.G. C?©sar, P.M. Cury, A.E. Silva
Apoptosis has an essential function in maintaining the integrity of the gastrointestinal mucosa. Its deregulation is associated with the occurrence of lesions such as in atrophic gastritis, peptic ulcers, intestinal metaplasia, and stomach tumorigenesis. Thus, the aim of the present study was to investigate the frequency of ap.. Read More»
- Genet. Mol. Res. 6(3):
Role of IL-10 and TNF-?± during Mycobacterium tuberculosis infection in murine alveolar macrophages
Authors: J. Shi, B.H. Sun, L.R. Zhou and X.S. Wang
Mycobacterium tuberculosis (Mtb) is known to be responsible for tuberculosis (TB), but the pathogenesis of this disease and the host defense mechanisms involved are, for the most part, poorly understood. In this study, we divided 30 male C57BL/6 mice into control and infection groups, and following injection with physiological saline or Mtb, respectively,.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15037819
- DOI:
- 10.4238/gmr.15037819
Screening and identification of cucumber germplasm and rootstock resistance against the root-knot nematode (Meloidogyne incognita)
Authors: X.Z. Li and S.X. Chen
Root-knot nematodes (Meloidogyne spp) are destructive agricultural pests that reduce the productivity of cultivated vegetables worldwide, especially when vegetables are cropped continuously in greenhouses. Cucumbers (Cucumis sativus L.), in particular, suffer extensive damage due to root-knot nematodes, and only a few wild spe.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029383
- DOI:
- 10.4238/gmr16029383
Phenotypic diversity of starch granules in cassava germplasm
Authors: L.M. Vasconcelos, A.C. Brito, C.D. Carmo, P.H.G.A. Oliveira and E.J. Oliveira
Demand for the development of cassava varieties with different native starches has guided the search for these characteristics in the germplasm of Manihot esculenta Crantz. Therefore, the objective of this study was to estimate the genetic diversity of cassava accessions for root and starch granule characteristics to guide the.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029276
- DOI:
- 10.4238/gmr16029276
Development of novel and polymorphic microsatellite DNA loci from Haliotis ovina
Authors: Z.B. Li, X.J. Tian, J.B. Shangguan, Y.Y. Cao, G.L. Zhang and L.N. Chen
Twelve microsatellite loci were developed from Haliotis ovina by the magnetic bead hybridization method. Genetic variability was assessed using 30 individuals from 3 wild populations. The number of alleles per locus ranged from 2 to 5, and the polymorphism information content ranged from 0.1228 to 0.6542. Observed and expected heterozygosities ranged from.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.26.6
- DOI:
- 10.4238/2015.January.26.6