Authors: T.-J. Xu, D.-Q. Sun, G. Shi and R.-X. Wang
Using an (AG)13 enriched genomic library of Mugil cephalus, 12 polymorphic microsatellite loci were isolated and characterized in a test population; the number of alleles ranged from 2 to 11. The observed and expected heterozygosities ranged from 0.2593 to 0.8966 and from 0.3047 to 0.8454, respectively. Two loci deviated from .. Read More»
Authors: F.M. Rodrigues, M.P.C. Telles, L.V. Resende, T.N. Soares, J.A.F. Diniz-Filho, A.T.A. J�¡como and L. Silveira
The maned wolf (Chrysocyon brachyurus) and the crab-eating fox (Cerdocyon thous) are two wild-canid species found in the Brazilian Cerrado. We tested cross-amplification and transferability of 29 short tandem repeat primers originally developed for cattle and domestic dogs and cats on 38 individuals of each of these two specie.. Read More»
Authors: Al-Seaf A, Keown JF and Van Vleck LD
Records of Holstein cows were used to examine how different models account for the effect of bovine somatotropin (bST) treatment on genetic evaluation of dairy sires for yield traits and somatic cell score. Data set 1 included 65,720 first-lactation records. Set 2 included 50,644 second-lactation records. Set 3 included 45,505.. Read More»
Authors: Al-Seaf A, KeownJF and Van Vleck LD
Records of Holstein cows from the Dairy Records Processing Center at Raleigh, NC were edited to obtain three data sets: 65,720 first, 50,694 second, and 65,445 later lactations. Correlations among yield traits and somatic cell score were estimated with three different models: 1) bovine somatotropin (bST) administration ignored.. Read More»
Authors: W. Jiang, H.Y. Ma, C.Y. Ma, S.J. Li, Y.X. Liu, Z.G. Qiao and L.B. Ma
The mud crab (Scylla paramamosain) is considered a potentially important marine crab species for selective breeding. Here, we first examined sex ratio and differences in 16 growth traits between females and males in a G1 population of S. paramamosain, and we then analyzed the correlation between these growth .. Read More»
Authors: Y.-H. Qi, W.-L. Yao, C.-H. Zhang and Y.-Q. Guo
This study investigated cadherin-1 (Cdh1) expression in the sensorimotor cortex of rats after spinal cord injury (SCI). The repairing effect of Cdh1 was evaluated by silencing its expression with lentivirus-mediated RNAi. Twenty male Sprague-Dawley (SD) rats were randomly divided into a normal group and an operation group. Rat.. Read More»
Authors: A.R.V.R. Horimoto, J.B.S. Ferraz, J.C.C. Balieiro and J.P. Eler
Models for estimation of frame scores in Nellore beef cattle (FRAME_GMA) were developed, comparing them with frame scores estimated using equations proposed by the Beef Improvement Federation (FRAME_BIF, USA). Correlation among frame scores obtained by these two methodologies, along with the independent variables considered in.. Read More»
Authors: S. Trivedi, H.S. Gehlot and S.R. Rao
In order to survive at high temperatures, thermophilic prokaryotes (Archaea and Eubacteria) adopt different strategies. Among several important contributing factors for stability of proteins are CGrich codons, the ratio of charged amino acids compared to uncharged amino acids, ionic interactions, amino acid preferences and the.. Read More»
Authors: P.R.L. Moreira and E. Morielle-Versute
Random amplified polymorphic DNA molecular marker was utilized as a means of analyzing genetic variability in seven bat species: Molossus molossus, M. rufus, Eumops glaucinus, E. perotis, Myotis nigricans, Eptesicus furinalis, and Artibeus planirostris. The determination of genetic diversity was based on 741 bands produced by .. Read More»
Authors: B. Yuan, X.Y. Li, T. Zhu, L. Yuan, J.P. Hu, J. Chen, W. Gao and W.Z. Ren
The gene for the nucleocapsid (N) protein of canine distemper virus was cloned into the pMD-18T vector, and positive recombinant plasmids were obtained by enzyme digestion and sequencing. After digestion by both EcoRI and KpnI, the plasmid was directionally cloned into the eukaryotic expression vector pcDNA; the positive clone pcDNA-N was screened by elec.. Read More»