Animal Genetics
Quantitative changes in mitochondrial DNA copy number in various tissues of pigs during growth
Authors: Y.M. Xie, L. Jin, X.J. Chen, M.N. He, Y. Wang, R. Liu, M.Z. Li and X.W. Li
Mitochondrial DNA (mtDNA) content is dependent on the energy requirements of tissues. To date, no comprehensive study has been conducted to examine mtDNA copy number variations in pigs. In the current study, quantitative polymerase chain reaction was performed to quantify the mtDNA copy number in 15 pig tissue types at 5 growth stages from embryo to adult.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.6.12
- DOI:
- 10.4238/2015.March.6.12
Phylogeny of major lineages of galliform birds (Aves: Galliformes) based on complete mitochondrial genomes
Authors: X.-Z. Kan, J.-K. Yang, X.-F. Li, L. Chen1, Z.-P. Lei, M. Wang, C.-J. Qian, H. Gao and Z.-Y. Yang
Complete mitochondrial DNA sequences have been used successfully to estimate phylogenetic relationships among animal taxa, and for studies of population genetics and molecular evolution. We made phylogenetic analyses of 22 species of Galliformes, with two species of Anseriformes as outgroups, using maximum likelihood (ML), max.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr898
- DOI:
- 10.4238/vol9-3gmr898
Cross-amplification tests of ungulate primers in the endangered Neotropical pampas deer (Ozotoceros bezoarticus)
Authors: M. Cosse, S. Gonz�¡lez and J.E. Maldonado
In cross-species amplification tests of 15 ungulate primers in pampas deer, five were retained to form a small panel of highly polymorphic loci that could be used to efficiently screen populations of this endangered species. The polymerase chain reactions were performed incorporating the universal fluorescent labeled M13 (-21).. Read More»
- Genet. Mol. Res. 6(4):
Hypoxia-related gene expression in porcine skeletal muscle tissues at different altitude
Authors: J. Zhang, L. Chen, K.R. Long and Z.P. Mu
Hypoxia influences many physiological processes, such as respiration, cardiovascular, neurophysiology, and digestion. Skeletal muscle is an important motor organ, which relies on oxygen of oxidation; however, the study of hypoxia in skeletal muscle is lacking. In order to understand the effect of hypoxia on skeletal muscle, we determined the expression le.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.28.10
- DOI:
- http://dx.doi.org/10.4238/2015.September.28.10
Expression and hypoxia adaptation analysis of the EPO gene in different tissues of plateau Tibetan pigs
Authors: B.Z. Deji, P. Shang, W.J. Danzeng, H. Zhang and Y.Z. Qiangba
This study aimed to observe the expression characteristics of the erythropoietin (EPO) gene in different tissues of Tibetan pigs and to explore the adaptation to hypoxic environments. The cDNA in heart, liver, lung, kidney, muscle, brain, and fat of Tibetan pigs was used as the template. Through the number of cycles of the polymerase chain reaction (PCR),.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.6.16
- DOI:
- 10.4238/2015.March.6.16
Morphological analysis and muscle-associated gene expression during different muscle growth phases of Megalobrama amblycephala
Authors: K.C. Zhu, D.H. Yu, J.K. Zhao, W.M. Wang and H.L. Wang
Skeletal muscle growth is regulated by both positive and negative factors, such as myogenic regulatory factors (MRFs) and myostatin (MSTN), and involves both hyperplasia and hypertrophy. In the present study, morphological changes during muscle development in Megalobrama amblycephala were characterized and gene expression levels were measured by quantitat.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.28.16
- DOI:
- http://dx.doi.org/10.4238/2015.September.28.16
The noggin2 gene of Gekko japonicus (Gekkonidae) is down-regulated in the spinal cord after tail amputation
Authors: Y. Liu, Y.L. Zhou, Y.Y. Qian, Y.J. Wang, F. Ding, X.S. Gu and M. Liu
The cDNA encoding noggin2 protein was obtained from the brain and spinal cord cDNA library of Gekko japonicus. The size of the noggin2 transcript and its expression in different tissues were analyzed by Northern blot analysis. In situ hybridization revealed positive hybridization signals in both gray and white matter of the sp.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr888
- DOI:
- 10.4238/vol9-3gmr888
Genetic parameters for earnings in Quarter Horse
Authors: A.P.A. Silva, R.A. Curi, B. Langlois and J.A.II.V. Silva
In this study, we estimated the heritability (h2) of earnings in the Quarter Horse in order to evaluate the inclusion of this trait in breeding programs. Records from 14,754 races of 2443 horses from 1978-2009 were provided by Sorocaba Hippodrome, São Paulo, Brazil. All ancestors of the registered horses were included i.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.1.2
- DOI:
- 10.4238/2014.August.1.2
Genetic diversity and classification of Tibetan yak populations based on the mtDNA COIII gene
Authors: Q.Q. Song, Z.X. Chai, J.W. Xin, S.J. Zhao, Q.M. Ji, C.F. Zhang, Z.J. Ma and J.C. Zhong
To determine the level of genetic diversity and phylogenetic relationships among Tibetan yak populations, the mitochondrial DNA cytochrome c oxidase subunit 3 (COIII) genes of 378 yak individuals from 16 populations were analyzed in this study. The results showed that the length of cytochrome c oxidase subunit 3 gene sequences was 781 bp, with nucleotide .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.13.3
- DOI:
- 10.4238/2015.March.13.3
Genetic diversity and population structure of Kazakh horses (Equus caballus) inferred from mtDNA sequences
Authors: M. Gemingguli, K.R. Iskhan, Y. Li, A. Qi, W. Wunirifu, L.Y. Ding and A. Wumaierjiang
The Kazakh horse is an important old horse breed in Xinjiang. They have contributed greatly to the breeding and improvement of other local horse breeds, yet their genetic diversity and population structure are not well understood. In the present study, we evaluated the genetic diversity of Kazakh horses and their relationship .. Read More»
- Genet. Mol. Res. 15(4):
- gmr.15048618
- DOI:
- 10.4238/gmr.15048618