Animal Genetics
Selecting representative microsatellite loci for genetic monitoring and analyzing genetic structure of an outbred population of orange tabby cats in China
Authors: X.Y. Du, S. Yi, X.Y. Huo, C. Wang, D.F. Liu, W.Z. Ren and Z.W. Chen
We optimized a panel of microsatellite markers from cat and tiger genetic data for efficient genetic monitoring and used it to analyze the genetic structure of an outbred cat stock in China. We selected a set of rich polymorphic microsatellite loci from 131 cat microsatellite loci and 3 Sumatran tiger microsatellite loci using agarose gel electrophoresis... Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.13.6
- DOI:
- 10.4238/2015.March.13.6
Use of PCR-DHPLC with fluorescence detection for the characterization of the bacterial diversity during cassava (Manihot esculenta crantz) fermentation
Authors: C.S. Kodama, S. Cuadros-Orellana, C.H.M.M. Bandeira, D.A. Gra�§as, A.S. Santos and A. Silva
Denaturing high-performance liquid chromatography (DHPLC) has been described as a suitable method to study DNA polymorphisms. Here, cassava (Manihot esculenta Crantz) fermentation liquor was examined using DHPLC analysis to characterize the bacterial diversity during the fermentation process. GC-clamped amplicons corresponding to a variable region of the .. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.February.28.2
- DOI:
- http://dx.doi.org/10.4238/2014.February.28.2
Genetic variability in maned wolf based on heterologous short-tandem repeat markers from domestic dog
Authors: Salim DC, Akimoto AA, Carvalho CB, Oliveira SF, Grisolia CK, Moreira JR and Klautau-Guimaraes MN
The maned wolf (Chrysocyon brachyurus) is the largest South American canid. Habitat loss and fragmentation, due to agricultural expansion and predatory hunting, are the main threats to this species. It is included in the official list of threatened wildlife species in Brazil, and is also protected by IUCN and CITES. Highly var.. Read More»
- Genet. Mol. Res. 6(2):
Genetic structure in Brazilian breeding colonies of the Roseate Spoonbill (Platalea ajaja, Aves: Threskiornithidae)
Authors: Mino CI and Del Lama SN
Roseate Spoonbills (Platalea ajaja, Linnaeus) are wading birds present in two of the most important Brazilian wetlands: the Pantanal wetlands and Rio Grande do Sul marshes. Natural populations of these species have not been previously studied with variable nuclear molecular markers. In order to support decision making regardin.. Read More»
- Genet. Mol. Res. 6(2):
Identification of 15 novel polymorphic microsatellite loci in pearl oyster (Pinctada fucata)
Authors: S.G. Fan, J.X. Wang, D.L. Zhang, Y.H. Guo and D.H. Yu
The pearl oyster Pinctada fucata is a commercially important marine shellfish. As a result, genetic improvement and selective-breeding program have been conducted for this species. Polymorphic microsatellites are effective molecular markers to investigate molecular marker-assisted selection and genetic variance. In this study,.. Read More»
- Genet. Mol. Res. 15(4):
- gmr.15048655
- DOI:
- 10.4238/gmr.15048655
Transferability of microsatellite loci from Cervidae species to the endangered Brazilian marsh deer, Blastocerus dichotomus
Authors: Leite KCE, Collevatti RG, Menegasso TR, Tomas WM and Duarte JMB
Blastocerus dichotomus, the marsh deer, is the largest Brazilian Cervidae species. The species is endangered because of hunting and loss of its natural habitat, i.e., flood plain areas, because of hydroelectric power station construction and agricultural land expansion. In the present study, we tested 38 microsatellite loci fr.. Read More»
- Genet. Mol. Res. 6(2):
In vitro differentiation of sperm from male germline stem cell
Authors: P. Zheng, X.W. Zhao, X.M. Zheng, A. Khalid, Q. Zhao and G.X. Zhang
In this study, we developed a model of sperm in vitro differentiation to study the mechanism of spermatogenesis. We isolated newborn male germ cells for in vitro differentiation. We found that after 4-5 weeks of culture, sperm-like cells were occasionally observed in cell co-culture and the feed-layer. After 1-2 weeks of culture followed by 4-5 weeks of c.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.10.5
- DOI:
- http://dx.doi.org/10.4238/2015.April.10.5
New evidence for nucleolar dominance in hybrids of Drosophila arizonae and Drosophila mulleri
Authors: C.I. Oliveira, H.E.M.C. Bicudo and M.M. Itoyama
Drosophila mulleri (MU) and D. arizonae (AR) are cryptic species of the mulleri complex, mulleri subgroup, repleta group. Earlier cytogenetic studies revealed that these species have different regulatory mechanisms of nucleolar organizing activity. In these species, nucleolar organizing regions are found in both the X chromoso.. Read More»
- Genet. Mol. Res. 5(4):
A novel polymorphism of the MYPN gene and its association with meat quality traits in Bos taurus
Authors: Y. Jiao, L.S. Zan, Y.F. Liu, H.B. Wang and B.L. Guo
Myopalladin (MYPN) is a multifunctional protein that maintains sarcomeric integrity and regulates Z-line structure. It is an important candidate gene for meat quality selection through marker-assisted selection. Using PCR-RFLP technology, we discovered a single-nucleotide polymorphism (SNP) (A1795G in exon 9) of the MYPN gene... Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr906
- DOI:
- 10.4238/vol9-3gmr906
Gene expression profiles of primary retinal pigment epithelial cells from apolipoprotein E knockout and human apolipoprotein E2 transgenic mice
Authors: D.H. Jo, J.H. Lee, H.J. Jun, J. Kim, Q. Wen, M.H. Hoang,Y.S. Yu, J.H. Kim and S.J. Lee
Age-related macular degeneration (AMD) causes visual impairment in the elderly. In non-neovascular AMD, studies involving human subjects have suggested potential involvement of aberrant lipid metabolism. However, there have been no reports on gene expression patterns in animal models of non-neovascular AMD with abnormal lipid metabolism such as apolipopro.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.13.14
- DOI:
- 10.4238/2015.March.13.14