Animal Genetics
Inexpensive metagenomic DNA extraction protocol with high quality from marine sediments contaminated by petroleum hydrocarbons
Authors: I. GarcÃ?Âa-Bautista, T. Toledano-Thompson, E. DantÃ?¡n-GonzÃ?¡lez, J. GonzÃ?¡lez-Montilla and R. Valdez-Ojeda
Marine environments are a reservoir of relevant information on dangerous contaminants such as hydrocarbons, as well as microbial communities with probable degradation skills. However, to access microbial diversity, it is necessary to obtain high-quality DNA. An inexpensive, reliable, and effective metagenomic DNA (mgDNA) extra.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039743
- DOI:
- 10.4238/gmr16039743
Full-length genomic and molecular characterization of Canine parvovirus in dogs from North of Brazil
Authors: S.P. Silva, L.N.P.P. Silva, E.D.L. Rodrigues, J.F. Cardoso, F.N. Tavares, W.M. Souza, C.M.P. Santos, F.M.S. Martins, I.S. Jesus, T.C. Brito, T.P.C. Moura, M.R.T. Nunes, L.M.N. Casseb, E. Silva Filho and A.R. Casseb
With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Belém city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Belém. Through sequencing o.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039719
- DOI:
- 10.4238/gmr16039719
A comparative study on genetic characteristics of two new varieties of Pelodiscus sinensis and their hybrid
Authors: H.Q. Zhang, X.J. Xu, Z.Y. He, J.Z. Shao, X.H. Zhang, Q.H. Meng and F.Y. Huang
Chinese soft-shelled turtle Pelodiscus sinensis has been an important aquaculture species in Southeast Asian countries. To breed a new variety of soft-shelled turtle with excellent properties and to evaluate the effect of hybridization of two turtle strains with a highly different trait phenotype, inheritance, microsatellite l.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039148
- DOI:
- 10.4238/gmr16039148
Isolation and characterization of polymorphic microsatellite markers for the chub mackerel (Scomber japonicus) and cross-species amplification in the blue mackerel (S. australasicus)
Authors: F.F. Chen, C.Y. Ma, L.P. Yan, H. Zhang, W. Wang, Y. Zhang and L.B. Ma
In this study, 10 polymorphic microsatellite markers were developed in Scomber japonicus and were examined on 30 individuals collected from the North Pacific. The number of alleles per locus ranged from 4 to 17. The observed and expected heterozygosities per locus ranged from 0.2759 to 0.8621 and from 0.43071 to 0.9177, respec.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039712
- DOI:
- 10.4238/gmr16039712
Association of CYP2C19*2 with hyperlipidemia in patients with the wild-type CYP2C9
Authors: K. Khalighi, G. Cheng, S. Mirabbasi, B. Khalighi, Y. Wu and W. Fan
Arachidonic acids are metabolized by cytochromes P450 (CYP450) to epoxyeicosatrienoic acids (EETs). EETs have many cardiovascular protective effects such as vasodilation, anti-hypertension, anti-inflammation, lipid-lowering effects, and favorable effects on glucose homeostasis. This study aimed to investigate whether gene poly.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039737
- DOI:
- 10.4238/gmr16039737
Alteration of -656(G/T) and -607(C/A) polymorphisms in interleukin-18 (IL-18) gene in house dust mite-sensitive allergic rhinitis patients in Thailand
Authors: A. Tungtrongchitr, J. Jumpasri, N. Sookrung, N. Visitsunthorn, P. Tantilipikorn, O. Piboonpocanan, N. Indrawattana, R. Tungtrongchitr and W. Chaicumpa
Allergic rhinitis (AR) is an IgE-mediated inflammation of the nasal membranes, which is naturally triggered by aeroallergens. House dust mites (HDM) are the most common inhalant allergens. Interleukin-18 (IL-18) has been established as an essential cytokine that can activate the generation of IgE. This randomized controlled st.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039641
- DOI:
- 10.4238/gmr16039641
Bayesian analyses of genetic parameters for growth traits in Nellore cattle raised on pasture
Authors: F.B. Lopes, J.L. Ferreira, R.B. Lobo and G.J.M. Rosa
This study was carried out to investigate (co)variance components and genetic parameters for growth traits in beef cattle using a multi-trait model by Bayesian methods. Genetic and residual (co)variances and parameters were estimated for weights at standard ages of 120 (W120), 210 (W210), 365 (W365), and 450 days (W450), and f.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039606
- DOI:
- 10.4238/gmr16039606
Promoter identification and analysis of key glycosphingolipid biosynthesis-globo series pathway genes in piglets
Authors: W.Y. Qin, L.N. Gan, R.W. Xia, W.H. Dong, S.Y. Sun, G.Q. Zhu,S.L. Wu and W.B. Bao
Glycosphingolipid biosynthesis-globo series pathway genes (FUT1, FUT2, ST3GAL1, HEXA, HEXB, B3GALNT1, and NAGA) play an important regulatory role in the defense against Escherichia coli F18 in piglets. In this study, we identified the transcription initiation site and promoter of this gene cluster by mined previous RNA-seq res.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029574
- DOI:
- 10.4238/gmr16029574
Genetic correlation and genome-wide association study (GWAS) of the length of productive life, days open, and 305-days milk yield in crossbred Holstein dairy cattle
Authors: P. Saowaphak, M. Duangjinda, S. Plaengkaeo, R. Suwannasing and W. Boonkum
In this study, we estimated the genetic parameters and identified the putative quantitative trait loci (QTL) associated with the length of productive life (LPL), days open (DO), and 305-day milk yield for the first lactation (FM305) of crossbred Holstein dairy cattle. Data comprising 4,739 records collected between 1986 and 20.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029091
- DOI:
- 10.4238/gmr16029091
Comparison of physical and chemical characteristics of collagen from the skin of cod (Gadus macrocephaius)
Authors: Y. Shu, H. Ren, R. Ao, W.C. Qi and Z.S. Zhang
Acid and pepsin-soluble collagen (ASC and PSC, respectively) were extracted from cod (Gadus macrocephaius) skin, and yields of 37.36 and 55.96% were obtained for ASC and PSC, respectively. The total yield of ASC and PSC was 93.92%, based on the lyophilized dry weight, which is higher than that obtained from other sources. Elec.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16027940
- DOI:
- 10.4238/gmr16027940