Isolation and characterization of polymorphic microsatellite markers for the chub mackerel (Scomber japonicus) and cross-species amplification in the blue mackerel (S. australasicus)
Accepted: November 30, -0001
Published: August 17, 2017
Genet.Mol.Res. 16(3): gmr16039712
In this study, 10 polymorphic microsatellite markers were developed in Scomber japonicus and were examined on 30 individuals collected from the North Pacific. The number of alleles per locus ranged from 4 to 17. The observed and expected heterozygosities per locus ranged from 0.2759 to 0.8621 and from 0.43071 to 0.9177, respectively. The polymorphism information content (PIC) was from 0.3931 to 0.8939. One locus showed moderate polymorphism (0.25 < PIC < 0.5), while the rest were highly polymorphic (PIC > 0.5). Two loci showed significant deviation from Hardy-Weinberg equilibrium after Bonferroni corrections (P < 0.005). No linkage disequilibrium was detected among the loci. Results of cross-species amplification showed that 10 microsatellite markers were successfully amplified in 29 individuals of S. australasicus and 9 indicated polymorphisms. These markers will be useful for investigating the genetic structure, gene flow, and species identification of S. japonicus and S. australasicus, its closely related species.
The chub mackerel (Scomber japonicus) is a widespread pelagic fish in the warm and temperate transition coastal areas and the adjacent seas of Atlantic, Pacific, and Northwest Indian (Collette and Nauen, 1983; Zeng et al., 2012). S. japonicus served as the global marine capture fishery production and provided stability for coastal systems (FAO, 2010). As one of the most important fishery resources of China, the yield of S. japonicus maintained at a higher level. However, the population was already in overfishing situation during the period 1980-2002 (Hua and Shan, 2004). Therefore, S. japonicus are more vulnerable to the impact of marine environment and strong fishing pressure. To protect this important fish resource, it is necessary to take knowledge of its population structure, gene flow levels, and genetic diversity (Utter, 2006; Cha et al., 2010). However, we know little about its ecological genetics and evolutionary biology.
Microsatellites, widely existing along the eukaryotic genome (Weber and May, 1989; O’Reilly and Wright, 1995), are popular genetic markers because of high polymorphism, ease of genotyping, and co-dominant inheritance (Sun et al., 2009). Moreover, they have been widely developed and used in the study of population genetics of many species (Guo et al., 2016; Mohanty et al., 2016; Tan et al., 2016). Furthermore, some of these microsatellite markers developed for Scomber species have been successfully used for amplification in other related species (Tang et al., 2009; Divya et al., 2016). However, it is not very sufficient, and some locus-by-population tests exhibited significant departure from Hardy-Weinberg equilibrium (HWE) (Cheng et al., 2015). So it is necessary to isolate more microsatellite markers for the utility of the population genetic study.
Scomber australasicus is one of the close species of S. japonicus and differ in a series of morphologic characters, including sculpturing of the skull, the number of precaudal vertebrae, the arrangement of palatine teeth, and the number of first dorsal spines (Matsui, 1967). S. australasicus inhabits the west Pacific and the southeast Indian Oceans.
It is known that the RAPD fragments contain rich microsatellites (Cifarelli et al., 1995; Ender et al., 1996). In the present study, we isolated 10 novel polymorphic microsatellite markers in S. japonicus using the RAPD-PCR method (Lunt, 1999). These markers provided a useful tool for investigating the genetic structure and species identification of S. japonicus and its closely related species.
Materials and Methods
Sample collection and DNA extraction
Our project was approved by the East China Sea Fisheries Research Institute. The 30 individuals of S. japonicus in the present study were collected from the North Pacific (145.383E, 41.083N). Moreover, 29 individuals of S. australasicus were collected from the Indian Ocean for the performance of cross-priming tests. Muscle tissues of specimens were preserved in 100% ethanol at room temperature until DNA extraction. Total Genomic DNA was extracted from muscles using the Animal Genomic DNA Extraction Kit (TianGen, Beijing, China) and stored at -20°C until RAPD was conducted.
Isolation of microsatellite markers
First, we randomly selected 10 RAPD primers from the catalogue of Sangon Biotech (Shanghai) Co., Ltd. (Table 1) to amplify the target DNA fragments. The PCR amplification was performed in a 25-μL total volume containing 12.5 μL 2X Kod PCR MasterMix, 0.5 μM RAPD primer (10 μM), 11 μL sterilized distilled water and approximately 1 μL template DNA. After 5 min of denaturation at 94°C, amplification proceeded for 40 cycles (94°C for 30 s, 37°C for 40 s, 72°C for 45 s) and a final extension for 5 min at 72°C. Then, the PCR products were cloned using the pMD19-T vector (Takara). The connection system including 1 μL pMD19-T Vector, 4 μL of PCR product, and 5 μL solution was stored at 16°C for reaction for 3 h and then transferred to 4°C. Finally, 10 μL of fluid connection was transformed into Escherichia coli competent cells (TianGen) and cultivated in plate culture medium at 37°C for several hours. The cultivated competent cells were randomly selected for sequencing using the ABI Prism 3730 automated DNA sequencer (PE Corporation). Microsatellite sequences obtained were searched using the SSR Hunter 1.3 software (Qiang and Wan, 2005).
|Primer name||Primer sequence (5'-3')|
Table 1. Ten RAPD primers used for amplifying the target DNA fragments of Scomber japonicus (the primers were derived from the product catalogue of Sangon Biotechnology Company).
Characteristics and polymorphism assessment
Firstly, seven individuals of S. japonicus were randomly selected to assess the preliminary polymorphism of the microsatellite markers. Moreover, we used 30 individuals of S. japonicus to quantify the levels of polymorphism of the 10 loci. The PCR amplification was performed on a Peltier Thermal Cycler in a 25-μL total volume, which included 0.75 μL of each primer (10 μM), 2.0 μL dNTP (2.5 μM), 2.5 μL 10X PCR buffer (Mg2+ plus), 2.5 U Taq polymerase, 17.5 μL sterilized distilled water, and approximately 1 μL template DNA under the following conditions: one cycle of denaturation at 94°C for 5 min; 30 cycles of 30 s at 94°C, 45 s at a primer-specific annealing temperature, and 1.5 min at 72°C. Finally, the products were extended for 7 min at 72°C (Table 1). The PCR products were separated on a 6% polyacrylamide denaturing gel and visualized by silver staining using a 10-bp DNA ladder marker pBR322/MspI (TianGen).
Allele frequency, observed (HO) and expected (HE) heterozygosities, the chi-square test for HWE, and the linkage disequilibrium (LD) were performed using the Popgene 3.4 software (Raymond and Rousset, 1995). The null-allele frequency was estimated by the MICROCHECKER version 2.2.3 software (Oosterhout et al., 2004). Polymorphism information content (PIC) was calculated by CERVUS 3.0 (Marshall et al., 1998). Significance values for all multiple tests were corrected by sequential Bonferroni procedure (Rice, 1989).
Results and Discussion
A total of 1000 clones were randomly selected to be tested by PCR with M13-47 (5'-GGG ATC CTC TAG AGA TT-3') and M13-48 (5'-GCC TGC AGG TCG ACG ATT-3'). Then, 148 positive clones were identified and sequenced using an ABI Prism 3730 automated DNA sequencer. In comparison to other species, the positive clone rate in this study was lower than that in fish (Cynoglossus semilaevis) (Liu et al., 2008) and crab (Scylla paramamosain) (Ma et al., 2010). Moreover, 75 pairs of primers were designed by using Primer Premier 5.0 and synthesized. The preliminary polymorphism assessment of the 75 pairs of microsatellite primers was evaluated by seven individuals, which were randomly picked out from the 30 individuals collected from the North Pacific (145.383E, 41.083N). All primers could lead to successfully PCR amplification, but only 10 of them showed polymorphism. Moreover, the levels of polymorphism of the polymorphic loci were evaluated by 30 individuals collected from the North Pacific (145.383E, 41.083N). Finally, we identified 10 polymorphic microsatellite loci (Table 2).
|Loci||GenBank accession No.||Primer sequence (5'-3')||Repeat motif||Ta (°C)||Allele size range (bp)|
|SJ-32||KY042110||F: CTCCCCTAACACTCCTTCCC||(GA)5… (GA)7…||55||291-325|
Table 2. Characteristics of the 10 microsatellite loci in the chub mackerel Scomber japonicus.
The levels of polymorphism of the 10 loci were qualified in 30 individuals of S. japonicus collected from the North Pacific (145.383E, 41.083N). A total of 99 alleles with size ranging from 135 to 325 bp were identified in 30 individuals. The NA per locus ranged from 4 to 17, with an average of 9.9. The HO and HE per locus ranged from 0.2759 to 0.8621 and from 0.4307 to 0.9177, with an average of 0.6226 and 0.7250, respectively. The PIC of these molecular markers was calculated to access their usefulness (Botstein et al., 1980). One locus (SJ-45) showed moderately polymorphic (0.25 < PIC < 0.5) and nine loci were highly polymorphic (PIC > 0.5).
Two loci (SJ-31 and SJ-65) showed significant departure from HWE after Bonferroni corrections (P < 0.005) (Rice, 1989), which may result from the small sample size or the presence of null alleles. The MICRO-CHECKER analysis showed no evidence for scoring error or technical or statistical artifacts (Oosterhout et al., 2004). There was no significant genotypic LD between all pairs of these loci after Bonferroni correction (P > 0.005). These 10 sequences were further searched in GenBank using the BLASTn program, and no similar sequence was found. The information about these pairs of primers is listed in Table 2.
The applicability of these markers in a closely related species, S. australasicus, was evaluated. All 10 loci were successfully amplified, and nine showed polymorphisms. The HO and HE ranged from 0.4286 to 1.0000 and from 0.5792 to 0.9273, respectively. The NA per locus ranged from 5 to 16 with an average of 9.4, and the PIC was from 0.4853 to 0.9039. Two loci (SJ-31and SJ-78) showed to be moderately polymorphic (0.25 < PIC < 0.5) and seven loci were highly polymorphic (PIC > 0.5) (Table 3). Six of the nine loci were detected to deviate from HWE. These microsatellite markers might be useful for detecting population genetic diversity and structure of the cross-species.
|Locus||Scomber japonicus||Scomber australasicus|
|NA||HO||HE||HWE (P value)||PIC||NA||HO||HE||HWE (P value)||PIC|
Table 3. Results of the 10 microsatellite loci in the Scomber japonicus population and the congeneric species, Scomber australasicus.
In conclusion, 10 polymorphic microsatellite markers were developed for S. japonicus by the RAPD-PCR method and amplified successfully in the related species S. australasicus. Most of these loci show high polymorphism in S. japonicus and S. australasicus. This set of microsatellite markers should be an important supplement of former microsatellite database and should also be used for accessing population genetic diversity and individual identification of S. japonicus and closely related species.
Research supported by the National Basic Research Special Foundation of China (#2013FY110700), the National Science and Technology Support Plan (#2013BAD13B05), the Special Fund for Agro-Scientific Research in the Public Interest (#201303047), and the National Infrastructure of Fishery Germplasm Resources.
About the Authors
Key Laboratory of the East China Sea and Oceanic F, East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai, China
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Key Laboratory of the East China Sea and Oceanic F, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China
- [email protected]
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