Animal Genetics
Preventive effect of tert-butylhydroquinone on scrotal heat-induced damage in mouse testes
Authors: Y.S. Li1, Y.G. Piao1, K. Nagaoka2,3, G. Watanabe2,3, K. Taya2,3 and C.M. Li1
To investigate the effect of tert-butylhydroquinone (tBHQ) on scrotal heat-induced damage in mice testes, 8-week-old mice were divided into 6 groups and administered with or without tBHQ through diet (10 mg/g), intraperitoneal injection (100 mg/kg body weight), or intratestis injection (12.5 mg/kg body weight), respectively. After single scrotal heat expo.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.11.5
- DOI:
- 10.4238/2013.November.11.5
Antibody-based detection of alkaline phosphatase in lepidopteran insects (Lepidoptera: Noctuidae)
Authors: R.A. Khan1, M. Rashid1, D. Wang2 and Y.L. Zhang1
Alkaline phosphatase (ALP) of Helicoverpa armigera Hub. (Lepidoptera; Noctuidae) (GenBank accession No. EU729322) was cloned and expressed. The target gene H.a-ALP, having an open reading frame of 1608 bp, was reverse-transcribed from cDNA by the polymerase chain reaction. The open reading frame of the target gene was cloned into the pET-32a expression ve.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.10.3
- DOI:
- 10.4238/2013.October.10.3
Genetic diversity and relationships among Chinese Eucommia ulmoides cultivars revealed by sequence-related amplified polymorphism, amplified fragment length polymorphism, and inter-simple sequence repeat markers
Authors: Y. Li, S.H. Wang, Z.Q. Li, C.F. Jin and M.H. Liu
Sequence-related amplified polymorphism (SRAP), amplified fragment length polymorphism (AFLP), and inter-simple sequence repeat (ISSR) markers were used to estimate the genetic diversity and relationships among Eucommia ulmoides cultivars in China. A total of 240, 192, and 150 DNA fragments were detected by 10 SRAP primer combinations, 10 AFLP primer comb.. Read More»
- Genet. Mol. Res. 13(4):
Expression of ADAMTS4 and ADAMTS5 in longissimus dorsi muscle related to meat tenderness in Nanyang cattle
Authors: X.H. Zhang, Y.X. Qi, X. Gao, J.Y. Li and S.Z. Xu
The ADAMTS4 and ADAMTS5 are secreted proteases, which can cleave aggrecan, brevican and versican to regulate rebuilding of the extracellular matrix. We analyzed the ADAMTS4 and ADAMTS5 gene expression patterns in longissimus dorsi muscle at intervals from 135 days fetal age to 30 months old by qRT-PCR in Nanyang cattle. Expression of ADAMTS4 was significa.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.2
- DOI:
- 10.4238/2013.October.18.2
Identification of spliced mRNA isoforms of retinoid X receptor (RXR) in the Oriental freshwater prawn Macrobrachium nipponense
Authors: Z. Li, W.Q. Wang, E.F. Zhang and G.F. Qiu
Retinoid X receptors (RXR) are members of the nuclear receptor family that are conserved from invertebrates to vertebrates, and they play an essential role in regulating reproductive maturation, molting, and embryo development. In this study, fi ve RXR isoforms, named RXRL2 (L, long form), RXRL3, RXRS1 (S, short form), RXRS2, .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.2
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.2
Characterization and cross-species amplification of microsatellite markers in African Silverbill (Lonchura cantans)
Authors: N.R. Parine1, D. Kumar2, A.A.K. Pathan1, M.S. Elrobh1, W. Khan1,3 and M. Alanazi1
We tested the cross-amplification of eight microsatellites developed for Bengalese finch in African Silverbill (Lonchura cantans). In order to develop resources for conservation genetic studies in the species L. cantans, we tested the amplification success and polymorphism in eight previously developed microsatellite loci, in L. cantans. All eight microsa.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.12
- DOI:
- 10.4238/2013.November.18.12
Polymorphic microsatellite loci isolated from Cervus unicolor (Cervidae) show inbreeding in a domesticated population of Taiwan Sambar deer
Authors: D.Y. Lin, T.Y. Chiang, C.C. Huang, H.D. Lin, S.J. Tzeng, S.R. Kang, H.M. Sung and M.C. Wu
Primers for eight microsatellites were developed; they successfully amplified DNA from 20 domesticated Formosan Sambar deer (Cervus unicolor swinhoei). All loci were polymorphic, with 10-19 alleles per locus. The average observed heterozygosity across loci and samples was 0.310, ranging from 0 to 0.750 at each locus. All loci .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.7
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.7
A new reliable reference gene UBA52 for quantitative real-time polymerase chain reaction studies in pyloric cecal tissues of the starfish Asterias rubens
Authors: A.F. Sadritdinova, A.A. Dmitriev, A.V. Snezhkina, M.S. Belenikin, G.S. Krasnov, O.G. Manylov, A.A. Kudryavtsev, N.V. Melnikova, A.S. Speranskaya, M.V. Darii, V.A. Lakunina, L.A. Uroshlev, A.O. Smurov, O.A. Stepanov and A.V. Kudryavtseva
The starfish Asterias rubens is one of the most abundant echinoderm species in the White, Barents, North, and Baltic Seas.This species is an important component of marine ecosystems and a model object for certain biological studies, in particular those requiring quantitative estimation of gene expression. As a rule, expression.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.8
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.8
Transcriptional responses in eastern honeybees (Apis cerana) infected with mites, Varroa destructor
Authors: Transcriptional responses in eastern honeybees (Apis cerana) infected with mites, Varroa destructor
The Varroa destructor mite has become the greatest threat to Apis mellifera health worldwide, but rarely causes serious damage to its native host Apis cerana. Understanding the resistance mechanisms of eastern bees against Varroa mites will help researchers determine how to protect other species from this organism. The A. cerana genome has not been previo.. Read More»
- Genet. Mol. Res. 13(4):
Association between metabolic syndrome and vascular endothelium dysfunction in children and adolescents
Authors: Y. Wei, G.L. Liu, J.Y. Yang, R.X. Zheng, L.H. Jiang, Y.P. Li and F.F. Gao
We aimed at investigating the association between metabolic syndrome (MS) and vascular endothelial cell dysfunction (ECD) in children and adolescents. Sixty children (30 obese children and 30 children with MS) were included in this retrospective analysis. Thirty healthy subjects were randomly selected as the control group. A series of indices/biomarkers k.. Read More»
- Genet. Mol. Res. 13(4):