Animal Genetics
Effect of phytosterols on rumen fermentation in vitro
Authors: Y.M. Xi, Z.H. Jin, L.J. Lin and Z.Y. Han
We investigated the effect of phytosterols on rumen fermentation in vitro using gas syringes as incubators. Phytosterols were dissolved in ethyl acetate (8.3%) and added at various concentrations to the common diet in rumen fluid. In vitro gas production (GP) was recorded after 3, 6, 12, 18, and 24 h incubation. Incubation was.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.16.12
- DOI:
- http://dx.doi.org/10.4238/2014.May.16.12
Molecular and biochemical characterization of the effects of insecticidal toxin from meloidae beetles on Helicoverpa armigera (Hub.) (Lepidoptera: Noctuidae)
Authors: R.A. Khan1, M. Rashid1, D. Wang2 and Y.L. Zhang1
The molecular and biochemical effects of an insecticidal toxin extracted from Meloidae beetles were investigated on Helicoverpa armigera. The toxin was identified as cantharidin, a well-known natural compound produced by beetles of family Meloidae and Oedemeridae. Furthermore, the effect of the toxin on the metabolic enzymes alkaline phosphatase (ALP) and.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.10.5
- DOI:
- 10.4238/2013.October.10.5
Expression of heat shock protein 90 alpha (Hsp90�±) in primary neonatal rat myocardial cells exposed to various periods of heat stress in vitro
Authors: A. Islam, B. Rehana, M. Zhang, Z.J. Liu, S. Tang, J. Hartung and E.D. Bao
The objective of this study was to investigate the mechanism of heat shock protein 90 alpha (Hsp90α) protection against heart damage resulting from heat stress by detecting Hsp90α mRNA, Hsp90α protein, protein localization, and cell damage in primary myocardial cells of neonatal rats in response to heat stres.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.14.9
- DOI:
- 10.4238/2014.April.14.9
Genetic characterization of Mytilus coruscus and M. galloprovincialis using microsatellite markers
Authors: J.H. Kang1, J.M. Lee2, E.S. Noh1, J.Y. Park1 and C.M. An1
Korean (hard-shelled) mussels (Mytilus coruscus) are an economically important endemic marine bivalve mollusk of Korea; yet, the population has rapidly declined because of overharvesting and habitat competition from the invasive Mytilus galloprovincialis species. The population structures of M. coruscus and M. galloprovincialis were analyzed by next-gener.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.13.2
- DOI:
- 10.4238/2013.November.13.2
Development of microsatellite markers for the kelp grouper Epinephelus bruneus by 454 pyrosequencing and transfer to related species
Authors: J.-H. Kang1, S.G. Yang2, T.S. Moon2, J.-Y. Park1 and T.-J. Choi3
The kelp or longtooth grouper (Epinephelus bruneus), which inhabits Eastern Asia, is the most economically important of 11 grouper species that inhabit the Southern Sea near Jeju Island in Korea. This species is listed as vulnerable by the International Union for the Conservation of Nature and Natural Resources because of a rapid decrease in its resources.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.13.1
- DOI:
- 10.4238/2013.November.13.1
Evolution of rbcL among Lathyrus and Kupicha�s classification
Authors: S. Marghali, N. Zitouna, M. Gharbi, I. Fadhlaoui and N. Trifi-Farah
Phylogenetic relationships in the Lathyrus genus were examined using cpDNA data, particularly data attributed to the “barcode” rbcL gene to construct a possible evolutionary scenario. Plant barcoding can be used to differentiate between species within a genus and to conserve DNA within the same species. We assessed the phylogeny of 29 species .. Read More»
- Genet. Mol. Res. 13(4):
Genetic diversity of locally adapted sheep from Pantanal region of Mato Grosso do Sul
Authors: B.A. Crispim1, A.B. Grisolia1, L.O. Seno2, A.A. Egito3, F.M. Vargas Junior2 and M.R. Souza2
Sheep of the Pantaneiro breed and seven other breeds, raised in the State of Mato Grosso do Sul, Brazil, were genotyped using eight microsatellite loci. The aim of the present study was to determine the genetic variability, phylogenetic relationship, and patterns of gene introgression and miscegenation among the animals surveyed, to obtain information abo.. Read More»
- Genet. Mol. Res. 12(4):
Study on the ERCC1 gene polymorphism response to chemotherapy and prognosis of gastric cancer
Authors: L. Liu1, C.H. Li2, T.F. Jin2 and D.Y. Xu
We conducted a cohort study to investigate the role of 2 single-nucleotide polymorphisms of the excision repair cross-complimentary group 1 (ERCC1) gene polymorphism in response to chemotherapy and clinical outcomes of gastric cancer. A total of 231 patients with newly diagnosed and histopathologically confirmed primary gastric cancer participated in the .. Read More»
- Genet. Mol. Res. 13(4):
Changes of gene expression profiles across different phases of vascular calcification in rats
Authors: Z.M. Jiang, X.J. Wu, Y. Liu, X.H. Du, S.J. Shen, L.Y. Xu,W.X. Sun
This study investigated the alteration of gene expression profiles in order to gain a deeper understanding into the molecular mechanism involved in different processes of vascular calcification (VC). Sprague Dawley (SD) rats were injected with 300,000 μg/kg vitamin D3 and gavaged with 25 mg/kg nicotine for 8 or 16 weeks to .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.26.4
- DOI:
- 10.4238/2013.November.26.4
Limitation of high-resolution melting curve analysis for genotyping simple sequence repeats in sheep
Authors: M. Yang, Y.J. Yue, T.T. Guo, J.L. Han, J.B. Liu, J. Guo, X.P. Sun, R.L. Feng, Y.Y. Wu, C.F. Wang, L.P. Wang and B.H. Yang
Variation in microsatellite or simple sequence repeat (SSR) loci has, until recently, relied heavily on the use of gel-based methods that can be both time consuming and difficult to genotype. Non gel-based systems are therefore important to increase simplicity and improve turn-around time without compromising assay sensitivity.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.8.7
- DOI:
- 10.4238/2014.April.8.7