Animal Genetics
Relative quantification of beta-casein expression in primary goat mammary epithelial cell lines
Authors: J. Ogorevc and P. Dov?
Primary mammary epithelial cell cultures were established from mammary tissue of lactating and non-lactating goats to assess the expression of beta-casein (CSN2) in vitro. Primary cell cultures were established by enzymatic digestion of mammary tissue and characterized using antibodies against cytokeratin 14, cytokeratin 18, and vimentin. The established .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.12
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.12
Verification of specific selection SNPs between broiler and layer chicken in Chinese indigenous chicken breeds
Authors: D. Lan, Y.D. Hu, Q. Zhu, D.Y. Li and Y.P. Liu
The direction of production for indigenous chicken breeds is currently unknown and this knowledge, combined with the development of chicken genome-wide association studies, led us to investigate differences in specific loci between broiler and layer chicken using bioinformatic methods. In addition, we analyzed the distribution of these seven identified lo.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.5
- DOI:
- 10.4238/2015.July.28.5
Genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China revealed by simple-sequence repeat markers
Authors: Y. Zhao, Y. Li, Y. Liu and Y.F. Yang
Simple-sequence repeat (SSR) markers were employed to assess the genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China. Fourteen SSR primer pairs generated a total of 94 alleles (90 were polymorphic, accounting for 95.74%), with a mean of 6.71 alleles per locus. The number of alleles detected at each locus ranged from 2 at BPPCT .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.7
- DOI:
- 10.4238/2015.July.28.7
Isolation and characterization of novel polymorphic microsatellite loci in Atrina vexillum Born (Pinnidae)
Authors: Y.S. Huang, Q.H. Li, Z.B. Li, J.B. Shangguan, Y.F. Ning and G. Dai
The pen shell, Atrina vexillum Born, is an edible shellfish that is widely consumed in the Asia-Pacific region. In this study, 11 polymorphic microsatellite loci were isolated from A. vexillum, and 30 wild individuals were used to evaluate the degree of polymorphism of these markers. The number of alleles per locus ranged from 3 to 8. The polymorphism inf.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.17.2
- DOI:
- http://dx.doi.org/10.4238/2015.April.17.2
DNA barcoding of Oryx leucoryx using the mitochondrial cytochrome C oxidase gene
Authors: K. Elmeer, A. Almalki, K.A. Mohran, K.N. AL-Qahtani and M. Almarri
The massive destruction and deterioration of the habitat of Oryx leucoryx and illegal hunting have decimated Oryx populations significantly, and now these animals are almost extinct in the wild. Molecular analyses can significantly contribute to captive breeding and reintroduction strategies for the conservation of this endang.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.8.2
- DOI:
- 10.4238/2012.March.8.2
Effect of Xin Mai Jia on atherosclerosis in rats
Authors: P. Li, G.P. Pan, M. Jia, Q.Q. Wang, Z.G. Guo, F.R. Zhao, G.L. Lei, G.R. Wan and G.M. Wan
We investigated the therapeutic effect of Xin Mai Jia (XMJ) on atherosclerosis (AS) in rats. Rat models of AS were established by peritoneally injecting vitamin D, feeding a high-fat diet, and inducing balloon injuries in rats. The stomachs of the rats were irrigated continuously for 10 weeks with XMJ. Blood lipid- and hemorheologyrelated indices of blood.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.19
- DOI:
- 10.4238/2015.June.1.19
Correlation analysis between single nucleotide polymorphism of DRD1 gene and stereotyped behavior of blue fox
Authors: Z.Y. Liu, E.J. Ren, H.X. Ba, Q. Wu, H.W. Zhu, X.M. Xing and F.H. Yang
This study was performed to investigate the correlation between stereotyped behavior of the blue fox and single nucleotide polymorphisms (SNPs) of the DRD1 gene. We choose the DRD1 gene as a major gene for investigating the correlation of gene polymorphism and self-biting disease by means of direct sequencing. Part of the DRD1 gene exon of the blue fox wa.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.8.1
- DOI:
- 10.4238/2015.June.8.1
DNA extraction from skins of wild (Hydrochoerus hydrochaeris and Pecari tajacu) and domestic (Sus scrofa domestica) species using a novel protocol
Authors: G.N. Ojeda, P.S. Amavet, E.C. Rueda and P.A. Siroski
Sometimes, commercial products obtained from wild animals are sold as if they were from domestic animals and vice versa. At this point of the productive chain, legal control of possible wildlife products is difficult. Common in the commerce of northern Argentina, skins of two wild species, the carpincho and the collared peccar.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.19.1
- DOI:
- 10.4238/2012.March.19.1
Association between polymorphisms in exons 4 and 10 of the BPI gene and immune indices in Sutai pigs
Authors: Z.C. Wu, Y. Liu, Q.H. Zhao, S.P. Zhu, Y.J. Huo, G.Q. Zhu, S.L. Wuand W.B. Bao
The bactericidal/permeability-increasing protein (BPI) gene has been identified as a candidate gene for disease-resistance breeding. We evaluated whether polymorphisms in exons 4 and 10 of the BPI gene are associated with immune indices [interleukin-2 (IL- 2), IL-4, IL-6, interferon-b (IFN-b), IL-10, and IL-12]. In this study, we identified one mutation (.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.8.2
- DOI:
- 10.4238/2015.June.8.2
Comparison between meat quality of Hanzhong White pigs and carcass weight
Authors: G.F. Wu, X.E. Shi, Y.F. Li, H.Z. Lu, C.C. Song, L. Jia and S.D. Sun
A total of 48 carcasses of crossbred Hanzhong White pigs were divided into 3 groups (I, 90-99 kg; II, 100-109 kg; III, 110- 119 kg) to investigate the influence of carcass weight on meat quality. The intramuscular fat content of the 3 groups increased from 1.90 to 4.90%; for meat color, Warner-Bratzler shear force, drip loss, and oxidation-type muscle fib.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.8.3
- DOI:
- 10.4238/2015.June.8.3