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Plant Genetics   Research Article

Assessing non-additive effects in GBLUP model

Authors: I.C. Vieira, J.P. R. dos Santos, L.P.M. Pires, B.M. Lima, F.M.A. Gon�§alves and M. Balestre

Understanding non-additive effects in the expression of quantitative traits is very important in genotype selection, especially in species where the commercial products are clones or hybrids. The use of molecular markers has allowed the study of non-additive genetic effects on a genomic level, in addition to a better understan.. Read More»

Genet. Mol. Res. 16(2):
gmr16029632
DOI:
10.4238/gmr16029632
Plant Genetics   Research Article

Genetic diversity in three natural populations of Pitcairnia flammea (l.) John (Bromeliaceae) estimated by ISSR markers

Authors: F.B. Souza-Sobreira, G.B. Souza, C.C.G. Rosado, F.D. Miranda, T.C.B. Soares, A.B.P.L. Gontijo

Bromeliads are greatly represented in the Atlantic Forest, although many species are threatened with extinction owing to habitat fragmentation and intense extraction for ornamental purposes. Therefore, it is necessary to conduct studies generating knowledge about genetic diversity and the distribution of this diversity among a.. Read More»

Genet. Mol. Res. 14(4):
2015.December.3.1
DOI:
10.4238/2015.December.3.1
Plant Genetics   Research Article

A set of novel microsatellite markers developed for an economically important tree, Dracontomelon duperreanum, in China

Authors: Z.F. Wang, H.L. Cao, L.F. Wu, Y. Guo, Q.M. Mei, M. Li, Y. Wang and Z.M. Wang

Dracontomelon duperreanum, the most representative species of the family Anacardiaceae, is an important multipurpose tree in China and Vietnam. However, no genetic diversity studies have been reported on this species. In this study, we identified 11 microsatellite markers for D. duperreanum by using the restriction-site-associ.. Read More»

Genet. Mol. Res. 16(2):
gmr16029578
DOI:
10.4238/gmr16029578
Human Genetics   Review Article

Backcross assisted by microsatellite markers in common bean

Authors: L.K. Oliveira1,3, L.C. Melo2, C. Brondani3, M.J.D. Peloso2 and R.P.V. Brondani3

The objectives of the present study were to monitor the effect of backcrossing through microsatellite markers and to compare different marker assisted selection strategies. Four populations were developed using donor parents resistant to the bean golden mosaic virus and, for all crosses, only individuals resistant to the bean golden mosaic virus were back.. Read More»

Genet. Mol. Res. 7(4):
Plant Genetics   Research Article

Molecular characterization and expression analysis of purple acid phosphatase gene from pearl oyster Pinctada martensii

Authors: Q.H. Wang, Y. Jiao, X.D. Du, X.X. Zhao, R.L. Huang,Y.W. Deng and F. Yan

Purple acid phosphatases (PAPs), also known as type 5 acid phosphatases, are widely present in animals, plants, and fungi. In mammal, PAP was reported to participate in immune defense and bone resorption. In this study, the characteristics and potential functions of a PAP gene from pearl oyster Pinctada martensii (pm-PAP) were examined. The Pm-PAP cDNA wa.. Read More»

Genet. Mol. Res. 14(1):
2015.January.26.10
DOI:
10.4238/2015.January.26.10
Human Genetics   Research Article

Mechanism of pingyangmycin-induced apoptosis of cultured human umbilical vein endothelial cells

Authors: Y.D. Huang, X. Tong, Q. Chen, S.W. Xia, Y. Zhuo, Z.H. Kang and X.Y. Xue

In this study, we investigated the effects of pingyangmycin (PYM) on the growth inhibition and apoptosis of human umbilical vein endothelial cells (HUVEC). In this study, we aimed to explore the optimal concentration of PYM to induce the apoptosis of HUVEC and to determine its mechanism of action. After treatment of HUVEC with.. Read More»

Genet. Mol. Res. 16(2):
gmr16029298
DOI:
10.4238/gmr16029298
Cytogenomics   Research Article

Conjugated linoleic acid-induced milk fat reduction associated with depressed expression of lipogenic genes in lactating Holstein mammary glands

Authors: L.Q. Han K. Pang H.J. Li S.B. Zhu L.F. Wang Y.B. Wang G.Q. Yang G.Y. Yang

The efficacy of conjugated linoleic acid (CLA) in diet supplements for milk fat reduction is well documented in several species. However, the mechanisms by which fatty acids regulate mammary lipogenesis remain largely unknown, especially with regard to gene expression of enzyme and regulators. In this study, 8 Holstein dairy cows in their mid-lactation pe.. Read More»

Genet. Mol. Res. 11(4):
2012.September.17.2
DOI:
10.4238/2012.September.17.2
Plant Genetics   Research Article

Relationship between male sterility and �²-1,3-glucanase activity and callose deposition-related gene expression in wheat (Triticum aestivum L.)

Authors: H.Z. Liu, G.S. Zhang, W.W. Zhu, Q.S. Ba, N. Niu, J.W. Wang,S.C. Ma and J.S. Wang

In previous studies, we first isolated one different protein β-1,3-glucanase using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry from normal wheat (Triticum aestivum L.) and chemical hybridization agent-induced male sterility (CIMS) wheat. In this experiment, β-1,3-glucanase acti.. Read More»

Genet. Mol. Res. 14(1):
2015.January.26.12
DOI:
10.4238/2015.January.26.12
Human Genetics   Research Article

Characterization and development of 56 EST-SSR markers derived from the transcriptome of Odontobutis potamophila

Authors: P.P. Wang, M. Ren, S.Q. Chen, S.W. Yin, C. Zhao, H.Y. Zhang, X.R. Li, Q.Q. Cao and G.Q. Zhou

Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequen.. Read More»

Genet. Mol. Res. 16(2):
gmr16029129
DOI:
10.4238/gmr16029129
Microbial Genetics   Research Article

Isolation and in silico functional analysis of MtATP6, a 6-kDa subunit of mitochondrial F1F0-ATP synthase, in response to abiotic stress

Authors: A.A. Moghadam S.M. Taghavi A. Niazi M. Djavaheri E. Ebrahimie

Mitochondrial F1F0-ATP synthase is a key enzymatic complex of energy metabolism that provides ATP for the cell. Subunits of this enzyme over-express under stress conditions. Little is known about the structure and regulatory mechanism of the F0 portion of this enzyme. We isolated the full-length coding sequence of the RMtATP6 gene from rice and wheat, and pa.. Read More»

Genet. Mol. Res. 11(4):
2012.October.4.3
DOI:
10.4238/2012.October.4.3