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Haplotype distribution of five nuclear genes based on network genealogies and Bayesian inference indicates that Trypanosoma cruzi hybrid strains are polyphyletic
Authors: L. Tomazi, S.Y. Kawashita, P.M. Pereira, B. Zingales andM.R.S. Briones
Chagas disease is still a major public health problem in Latin America. Its causative agent, Trypanosoma cruzi, can be typed into three major groups, T. cruzi I, T. cruzi II and hybrids. These groups each have specific genetic characteristics and epidemiological distributions. Several highly virulent strains are found in the h.. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2gmr591
- DOI:
- 10.4238/vol8-2gmr591
A rapid and efficient method for isolation of total RNA from Euglena gracilis (Euglenoidea)
Authors: Euglena gracilis; Sodium dodecyl sulfate-Tris;RNA isolation; Reverse transcription-polymerase chain reaction
RNA isolation is essential to the study of gene expression at the molecular level. However, it is difficult to isolate RNA from organisms that contain large amounts of polysaccharides or other compounds that bind or coprecipitate with RNA, such as the unicellular protist Euglena gracilis. Currently, there is no commercial kit .. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2gmr590
- DOI:
- 10.4238/vol8-2gmr590
�±-Thalassemia (3.7 kb deletion) in a population from the Brazilian Amazon region: Santar�©m, Par�¡ State
Authors: A.E.S. Souza, G.L. Cardoso, S.Y.L. Takanashi and J.F. Guerreiro
The ethnic composition of the Brazilian population favors high frequencies of the -α3.7 deletion, responsible for α-thalassemia, because this mutation is very common in African populations. In spite of its importance, this hemoglobinopathy has been poorly investigated in Brazil, especially at the molecular level. W.. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2gmr601
- DOI:
- 10.4238/vol8-2gmr601
Construction of a lentiviral vector encoding heme oxygenase 1 and its introduction into mouse adipose tissue-derived stem cells
Authors: C.H. Zhu, W. Lei and Z.R. Chen
Many studies exist concerning the use of stem cells as delivery vehicles in gene therapy, expressing genes such as vascular endothelial growth factor 165 and hepatocyte growth factor. However, few reports regarding adipose tissue-derived stem cells (ADSCs) and the heme oxygenase 1 (HO-1) gene have been published. Therefore, we established a lentiviral vec.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.9.10
- DOI:
- http://dx.doi.org/10.4238/2015.September.9.10
Mendelian inheritance, genetic linkage, and genotypic disequilibrium for nine microsatellite loci in Cariniana estrellensis (Raddi) Kuntze (Lecythidaceae)
Authors: T.Y.K. Kubota, A.M. Silva, J. Cambuim, A.A. Silva, S. Pupin, M.S. Silva, M.A. Moraes, M.L.T. Moraes and A.M. Sebbenn
Cariniana estrellensis is one of the largest trees found in Brazilian tropical forests. The species is typical of advanced stages of succession, characteristic of climax forests, and essential in genetic conservation and environmental restoration plans. In this study, we assessed Mendelian inheritance, genetic linkage, and gen.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029653
- DOI:
- 10.4238/gmr16029653
Screening of differentially expressed genes in pathological scar tissues using expression microarray
Authors: L.P. Huang, Z. Mao, L. Zhang, X.X. Liu, C. Huang and Z.S. Jia
Pathological scar tissues and normal skin tissues were differentiated by screening for differentially expressed genes in pathologic scar tissues via gene expression microarray. The differentially expressed gene data was analyzed by gene ontology and pathway analyses. There were 5001 up- or down-regulated genes in 2-fold differentially expressed genes, 956.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.9.13
- DOI:
- http://dx.doi.org/10.4238/2015.September.9.13
Association of vitamin D-binding protein variants with chronic obstructive pulmonary disease: a meta-analysis
Authors: Y.L. Wang, H. Kong, W.P. Xie and H. Wang
Gene polymorphism of vitamin D-binding protein (VDBP) correlates with chronic obstructive pulmonary disease (COPD), but the results remain inconclusive. We aimed to explore the association between VDBP gene polymorphism and COPD. We searched MEDLINE, Embase, Web of Science, and China National Knowledge Infrastructure for publications addressing the associ.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.9.16
- DOI:
- http://dx.doi.org/10.4238/2015.September.9.16
Movement patterns and genetic diversity of wild and reintroduced common dormice, Muscardinus avellanarius
Authors: D.M. Naim, S. Telfer, S. Tatman, S. Bird, S.J. Kemp and P.C. Watts
Movement is an important life history trait that can have an impact on local adaptation, and other evolutionary phenomena. We used a combination of nestbox survey data and genetic techniques (genotyping at 10 microsatellite loci) to quantify patterns of movement in common dormice Muscardinus avellanarius at two distinct sites .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.10.8
- DOI:
- 10.4238/2014.January.10.8
Contribution of morphoagronomic traits to grain yield and earliness in grain sorghum
Authors: K.J. da Silva, P.E. Teodoro, C.B. de Menezes, M.P.M. J�ºlio,V.F. de Souza, M.J. da Silva, L.D. Pimentel and A. Bor�©m
Given the importance of selecting lines to obtain hybrids, we aimed to verify the relationship between morphological traits that can be used as the criteria for the selection of sorghum lines with high grain yield and earliness. A total of 18 traits were evaluated in 160 sorghum elite lines, in an incomplete block design with .. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029649
- DOI:
- 10.4238/gmr16029649
Isolation and characterization of polymorphic microsatellite markers in Bagarius yarrelli using RNA-Seq
Authors: M. Du, Y.H. Liu and B.Z. Niu
The yellow sisorid catfish (Bagarius yarrelli) is a vulnerable fish species. In this study, seven polymorphic microsatellite DNA markers for yellow sisorid catfish were described, using RNA-Seq methodology. In B. yarrelli (N = 44) from a Hekou wild population, allelic frequency, and observed and expected heterozygosities per locus varied from two to six, .. Read More»
- Genet. Mol. Res. 14(4):
- 2015
- DOI:
- 10.4238/2015