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Analysis of allopolyploidy-induced rapid genetic and epigenetic changes and their relationship in wheat
Authors: T. Qiu, Y.Z. Dong, X.M. Yu, N. Zhao and Y.F. Yang
We used the conventional and methylation-sensitive randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analyses to assess genome-wide changes and explore the relationships between genetic and epigenetic variations among individuals of a newly synthesized allohexaploid wheat line whose genomic cons.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029303
- DOI:
- 10.4238/gmr16029303
Comparison of DNA extraction methods for polymerase chain reaction amplification of guanaco (Lama guanicoe) fecal DNA samples
Authors: M.I. Espinosa, A. Bertin, F.A. Squeo, A. Cort�©s and N. Gouin
Feces-based population genetic studies have become increasingly popular. However, polymerase chain reaction (PCR) amplification rates from fecal material vary depending on the species, populations, loci, and extraction protocols. Here, we assessed the PCR amplification success of three microsatellite markers and a segment of the mitochondrial control regi.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.13
- DOI:
- 10.4238/2015.January.23.13
Correlation of acetabular chondrocyte apoptosis with caspase-3 and Bcl-2 expression in developmental dislocations of the hip
Authors: L.J. Ding, Y.L. Liu, G. Ma, Y.Q. Jia, Y.S.H. Wei and X.M. Liu
This study aimed to determine whether abnormal apoptosis is present in acetabular cartilage in early developmental dislocations of the hip (DDH), and if so, whether it is correlated with the expression of caspase-3 and Bcl-2. DDH was induced in 24 4-week-old New Zealand white rabbits. Acetabular cartilage specimens from the experimental and control groups.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15036809
- DOI:
- 10.4238/gmr.15036809
Genomic characterization and phylogenetic analysis of Chinese sacbrood virus isolated from Loess Plateau, China
Authors: H. Yu, T.X. Liu and D. Wang
The complete genomic RNA of the Chinese sacbrood virus (CSBV) strain, which infects the honeybees in the Loess plateau, was sequenced and analyzed. The CSBV-SX strain contains 8705 nucleotides, which includes a single large open reading frame (99-8681 nucleotides) encoding 2860 amino acids. A novel efficient identification method was used to investigate t.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15036928
- DOI:
- 10.4238/gmr.15036928
Novel NPHS1 splice site mutations in a Chinese child with congenital nephrotic syndrome
Authors: R. Fu, M.F. Gou, W.H. Ma, J.J. He, Y. Luan and J. Liu
Congenital nephrotic syndrome (CNS) is defined as heavy proteinuria or nephrotic syndrome occurring before 3 months of age. It is characterized by early onset and progresses to end-stage renal disease. Recently, several genes associated with CNS have been identified, including NPHS1 and NPHS2. Mutations in the NPHS1 gene have been identified in patients w.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.17
- DOI:
- 10.4238/2015.January.23.17
Isolation and characterization of microsatellite loci in Branchiostoma belcheri Gray (Amphioxus)
Authors: Z.B. Li, Y.S. Huang, J.B. Shangguan, Y.F. Ning, Y. Yi and G. Dai
Branchiostoma belcheri Gray is a second-class, nationally protected protochordate in China. We developed 10 novel polymorphic sites in B. belcheri, which were examined using a population of 30 wild individuals from Xiamen, China. The polymorphism information content ranged between 0.141 and 0.681, and the number of alleles varied from 2 to 5. The expected.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.August.28.5
- DOI:
- http://dx.doi.org/10.4238/2015.August.28.5
Isolation and characterization of microsatellite loci for Cunninghamia lanceolata (Lamb.) Hook
Authors: Y.X. Li, Z.S. Wang, J.K. Sui, Y.F. Zeng, A.G. Duan and J.G. Zhang
As a result of human activities, wild populations of Cunninghamia lanceolata (Cupressaceae) have sharply declined in recent years. The development and implementation of a valid conservation strategy require a clear understanding of the genetic makeup of this species. Eleven polymorphic microsatellite loci were isolated and characterized from samples of 52.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.19
- DOI:
- 10.4238/2015.January.23.19
Duplication polymorphisms in exon 4 of �º-casein gene in yak breeds/populations
Authors: S. Pingcuo, J. Gao, Z.R. Jiang, S.Y. Jin, C.Y. Fu, X. Liu, L. Huang and Y.C. Zheng
The objective of this study was to compare 12 bp-duplication polymorphisms in exon 4 of the κ-casein gene among 3 breeds/populations of yak (Bos grunniens). Genomic DNA was extracted from yak blood or muscle samples (N = 211) and a partial sequence of exon 4 of κ-casein gene was amplified by polymerase chain reaction. A polyacrylamide gel elec.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.August.28.8
- DOI:
- http://dx.doi.org/10.4238/2015.August.28.8
Integrated microRNA-mRNA analysis of pancreatic ductal adenocarcinoma
Authors: P.F. Liu, W.H. Jiang, Y.T. Han, L.F. He, H.L. Zhang and H. Ren
The main aim of this study was to explore the underlying molecular mechanisms and potential target molecules of pancreatic adenocarcinoma. The miRNA (GSE32678) and mRNA (GSE32676) expression profiles of patients with pancreatic ductal adenocarcinoma and healthy controls were downloaded from the Gene Expression Omnibus database. Differentially expressed mi.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.August.28.14
- DOI:
- http://dx.doi.org/10.4238/2015.August.28.14
First transcriptional survey of the Malpighian tubules of giant mealworm, Zophobas morio (Coleoptera: Tenebrionidae)
Authors: J.R. Silva, R.A. Prado, D.T. Amaral and V.R. Viviani
The Malpighian tubules play a key role in insect osmoregulation. Although a transcriptional analysis has been done for the Malpighian tubules in Drosophila melanogaster (Diptera), no functional genomics analysis has yet been carried out for any Coleoptera species. Recently, we constructed a cDNA library from Malpighian tubules of larval Zophobas morio, a .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.21
- DOI:
- 10.4238/2015.January.23.21