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Plant Genetics   Research Article

Diallel analysis of leaf disease resistance in inbred Brazilian popcorn cultivars

Authors: R.A. Vieira, C.A. Scapim, L.M. Moterle, D.J. Tessmann, T.V. Conrado and A.T. Amaral J�ºnior

We estimated general and specific combining abilities and examined resistance to northern leaf blight (Exserohilum turcicum) and to gray leaf spot (Cercospora zeae-maydis) in a set of nine inbred popcorn lines. These inbreds were crossed in a complete diallel scheme without reciprocals, which produced 36 F1 hybrids. Two experi.. Read More»

Genet. Mol. Res. 8(4):
vol8-4gmr655
DOI:
10.4238/vol8-4gmr655
Plant Genetics   Research Article

Molecular identification of the traditional herbal medicines, Arisaematis Rhizoma and Pinelliae Tuber, and common adulterants via universal DNA barcode sequences

Authors: B.C. Moon, W.J. Kim, Y. Ji, Y.M. Lee, Y.M. Kang and G. Choi

Methods to identify Pinelliae Tuber and Arisaematis Rhizoma are required because of frequent reciprocal substitution between these two herbal medicines and the existence of several closely related plant materials. As a result of the morphological similarity of dried tubers, correct discrimination of authentic herbal medicines is difficult by conventional .. Read More»

Genet. Mol. Res. 15(1):
gmr.15017064
DOI:
10.4238/gmr.15017064
Animal Genetics   Research Article

Evaluation of methods of DNA extraction from Staphylococcus aureus in milk for use in real-time PCR

Authors: A.G. Dibbern, B.G. Botaro, M.P. Viziack, L.F.P. Silva and M.V. Santos

The aim of this study was to evaluate the repeatability and performance of 4 methods of extracting DNA from Staphylococcus aureus (SAU) and the gene encoding bovine mitochondrial cytochrome B (BMCB) in milk samples from cows with subclinical mastitis for use in amplification by real-time polymerase chain reaction. Two milk samples were obtained from cows .. Read More»

Genet. Mol. Res. 14(1):
2015.January.16.6
DOI:
10.4238/2015.January.16.6
Bioinformatics   Short commentary

Molecular conservation of the mammalian leptin protein

Authors: J.E. Gabriel and K.C.F. Lidani

In this study, we comparatively assessed multiple sequences of the leptin protein from different animal species to establish new insights into conservation degree of biological sequences and evolutionary biology among mammals using computational biology tools. First, amino acid sequences of the leptin protein from Homo sapiens (human, P41159), Sus scrofa .. Read More»

Genet. Mol. Res. 14(1):
2015.January.16.9
DOI:
10.4238/2015.January.16.9
Plant Genetics   Research Article

Genetic control and transgressive segregation of zinc, iron, potassium, phosphorus, calcium, and sodium accumulation in cowpea (Vigna unguiculata) seeds

Authors: C.A. Fernandes Santos1 and L.S. Boiteux2

Cowpea crop, through combining a range of essential minerals with high quality proteins, plays an important role in providing nutritional security to human population living in semi-arid regions. Studies on genetics of biofortification with essential minerals are still quite scarce, and the major objective of the present study was to provide genetic informat.. Read More»

Genet. Mol. Res. 14(1):
2015.January.16.10
DOI:
10.4238/2015.January.16.10
Plant Genetics   Research Article

Genetic diversity of Saccharum officinarum accessions in Pakistan as revealed by random amplified polymorphic DNA

Authors: F.A. Khan, A. Khan, F.M. Azhar and S. Rauf

Genetic diversity of 20 sugarcane accessions in Pakistan was studied using 21 random amplified polymorphic DNA markers. The mean genetic distance between the cultivars was 39.03%, demonstrating that a large part of the genome is similar among the accessions. This probably arises from a lack of parental diversity, with few clon.. Read More»

Genet. Mol. Res. 8(4):
Microbial Genetics   Research Article

Microsatellite markers linked to the locus of the watermelon fruit stripe pattern

Authors: R.N.C.S. Gama, C.A.F. Santos, R.C.S. Dias, J.C.S.F. Alves and T.O. Nogueira

Agronomic performance and external and internal appearance of watermelon (Citrullus lanatus) fruit are important traits that should be taken into consideration during the development of a new cultivar, as well as being the principal identification elements used by the consumer, which are based on the external appearance and quality of the fruit. Externally, .. Read More»

Genet. Mol. Res. 14(1):
2015.January.16.11
DOI:
10.4238/2015.January.16.11
Human Genetics   Research Article

Effect of pregnancy on the proliferation of rat adipose-derived stem cells

Authors: J.N. Li, Y. Zhang, Y.F. Wang and J.Y. Chen

Stem cell therapy faces many problems including poor survival rates and low viability. Enhancing the biological functions of stem cells improves efficacy of therapies. Estrogen, whose levels are elevated during pregnancy, affects the properties of bone marrow mesenchymal stem cells. Evidence suggests that adipose-derived stem .. Read More»

Genet. Mol. Res. 16(1):
gmr16019059
DOI:
10.4238/gmr16019059
Human Genetics   Research Article

Association of single nucleotide polymorphisms with carcass traits in Nellore cattle

Authors: J.B.S. Ferraz, L.F.B. Pinto, F.V. Meirelles, J.P. Eler, F.M. de Rezende, E.C.M. Oliveira, H.B. Almeida, B. Woodward4 and D. Nkrumah

The association between two single nucleotide polymorphisms (SNPs), T945M and UCP1SNP1, with hot carcass weight (HCW, kg, N = 618), longissimus dorsi muscle area (REA, cm2, N = 633), and backfat thickness (BF, mm, N = 625), measured in Nellore cattle in Brazil, was evaluated. Likelihood ratio tests were used to evaluate reduce.. Read More»

Genet. Mol. Res. 8(4):
vol8-4gmr650
DOI:
10.4238/vol8-4gmr650
Human Genetics   Research Article

GRK2 desensitizes flow-induced responses in osteoblasts

Authors: Y. Xing, Y. Gu, X. Shan, L. Wang and J. You

Bone desensitization after mechanical loading is essential for bone to adapt to its mechanical environment. However, the desensitization mechanism is unknown. Previous studies suggest that G protein-coupled receptors (GPCRs), including P2Y and parathyroid hormone receptors, play important roles in osteoblast mechanobiology. Th.. Read More»

Genet. Mol. Res. 16(1):
gmr16019363
DOI:
10.4238/gmr16019363