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Comparative fusion expression of maize SINAT5 in two different strains of Escherichia coli
Authors: A. Gholizadeh
SINAT5 is a plant E3 ligase that regulates auxin signaling and root morphogenesis by ubiquitination of the NAC1 protein. Consequently, it may be a putative regulator of aspects of plant development cycles that are controlled by auxin. Efficient production, purification and correctly folded form of this protein are important requirements for functional stu.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.24.1
- DOI:
- 10.4238/2012.August.24.1
Myostatin (GDF8) single nucleotide polymorphisms in Nellore cattle
Authors: A.B. Grisolia1,2, G.T. D�Angelo1, L.R. Porto Neto1, F. Siqueira3 andJ.F. Garcia1
The myostatin gene, also known as GDF8 (growth differentiation factor 8), is located on bovine chromosome 2 (BTA2); it has three exons and two introns. Myostatin is specifically expressed during embryonic development and in adult skeletal muscle, functioning as a negative regulatory protein. Several cattle breeds (Piedmontese, Belgian Blue .. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr548
- DOI:
- 10.4238/vol8-3gmr548
New microsatellite markers for the abalone Haliotis midae developed by 454 pyrosequencing and in silico analyses
Authors: R. Slabbert J.-A. Hepple C. Rhode A.E. Bester-Van der Merwe R. Roodt-Wilding
Farming of Haliotis midae is the most lucrative aquaculture venture in South Africa. The genome of this species needs to be studied to assist in selective breeding programs aimed at increasing overall yield, and molecular markers will be required to attain this goal. We identified and characterized 82 polymorphic microsatellite loci by using repeat-enrich.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.24.2
- DOI:
- 10.4238/2012.August.24.2
Knockdown expression of Apc11 leads to cell-cycle distribution reduction in G2/M phase
Authors: Y.-J. Shi K.-K. Huo
Anaphase-promoting complex/cyclosome (APC/C) is a key E3 ubiquitin ligase in cell division, which catalyses ubiquitination of cell-cycle regulators. Studying this complex could reveal important information regarding its application in cancer research and therapy. In this study, 4 synthesized small interfering RNAs (siRNAs) were transfected into HEK293T ce.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.24.6
- DOI:
- 10.4238/2012.August.24.6
A method for extracting period~10 modulations from DNA sequence correlations applied to the Drosophila melanogaster genome
Authors: J.C.O. Guerra P. Licinio
Nucleosome DNA packaging and positioning within the Drosophila melanogaster genome imposes a weak modulation, with a period of about 10 bp in the genomic composition correlations. We present formalism for extracting such modulations from an irreducible set of six correlation functions calculated along the D. melanogaster genome. These modulations were see.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.24.8
- DOI:
- 10.4238/2012.August.24.8
Population genetic analysis and conservation strategies for redtail shrimp Fenneropenaeus penicillatus using ten microsatellite markers
Authors: Y.Y. Cao, J.B. Shangguan, Z.B. Li
Fenneropenaeus penicillatus, which is on the Red List of Endangered Species for China, is an important shrimp species. However, there is not enough genetic information on F. penicillatus for conservation and management purposes. Ten microsatellite markers were used to analyze the genetic diversity, genetic differentiation, and.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019068
- DOI:
- 10.4238/gmr16019068
Single nucleotide polymorphisms from Theobroma cacao expressed sequence tags associated with witches� broom disease in cacao
Authors: L.S. Lima1, K.P. Gramacho1, N. Carels2, R. Novais1, F.A. Gaiotto2,U.V. Lopes1, A.S. Gesteira2, H.A. Zaidan1,2, J.C.M. Cascardo2,J.L. Pires1 and F. Micheli2,3
In order to increase the efficiency of cacao tree resistance to witches’ broom disease, which is caused by Moniliophthora perniciosa (Tricholomataceae), we looked for molecular markers that could help in the selection of resistant cacao genotypes. Among the different markers useful for developing marker-assisted selection, single nucleotide polymorp.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr603
- DOI:
- 10.4238/vol8-3gmr603
Lack of association between CARD10/CARMA3 tag SNPs and psoriasis vulgaris in the southern Chinese population
Authors: G. Shi, M.F. Zhang, P.Y. Liao, T.T. Wang, S.J. Li, Y.M. Fan, K.J. Zhu
Previously, we determined that the CARD11 rs4722404 single nucleotide polymorphism (SNP) increases risk of early-onset psoriasis vulgaris (PsV). Moreover, the CARD14 gene polymorphism c.C2458T (p.Arg820Trp) is associated with clinical features of this disease. CARMA1/CARD11, CARMA2/CARD14, and CARMA3/CARD10 are conserved acros.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019025
- DOI:
- 10.4238/gmr16019025
Differences in mitochondrial gene expression profiles, enzyme activities and myosin heavy chain types in yak versus bovine skeletal muscles
Authors: Y.Q. Lin Y.O. Xu Y. Yue S.Y. Jin Y. Qu F. Dong Y.P. Li Y.C. Zheng
Hypoxia can affect energy metabolism. We examined gene expression and enzyme activity related to mitochondrial energy metabolism, as well as myosin heavy chain (MyHC) types in yaks (Bos grunniens) living at high altitudes. Real-time quantitative PCR assays indicated that the yak has significantly lower levels of carnitine palmitoyltransferase (CPT) mRNA i.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.22.3
- DOI:
- 10.4238/2012.May.22.3
Selecting black-spot resistant papaya genotypes derived from backcrossing and hybrids
Authors: T.P.S. Poltronieri, S.F. Silveira, M. Vivas, R. Santa Catarina,D.F.M. Cortes, A.O.N. Azevedo and M.G. Pereira
Papaya crop is important to Brazilian agribusiness. However, the expansion of papaya cultivation in the country is affected by the absence of commercial cultivars presenting good disease resistance. The black-spot caused by the fungus Asperisporium caricae is the most damaging foliar disease affecting Brazilian papaya crops. T.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019401
- DOI:
- 10.4238/gmr16019401