Se
In silico identification and analysis of phytoene synthase genes in plants
Authors: Y. Han, Q.S. Zheng, Y.P. Wei, J. Chen, R. Liu and H.J. Wan
In this study, we examined phytoene synthetase (PSY), the first key limiting enzyme in the synthesis of carotenoids and catalyzing the formation of geranylgeranyl pyrophosphate in terpenoid biosynthesis. We used known amino acid sequences of the PSY gene in tomato plants to conduct a genome-wide search and identify putative candidates in 34 sequenced plan.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.5
- DOI:
- 10.4238/2015.August.14.5
Association of TIRAP (MAL) gene polymorhisms with susceptibility to tuberculosis in a Chinese population
Authors: Y.X. Zhang, Y. Xue, J.Y. Liu, M.Y. Zhao, F.J. Li, J.M. Zhou, H.J. Wang and J.C. Li
Toll-interleukin 1 receptor (TIR) domain containing adaptor protein (TIRAP; also known as MAL) is an essential adaptor molecule in Toll-like receptor signaling, involved in activating the innate immune response during infection. Genetic variations in the TIRAP gene may influence human susceptibility to infectious disease. To d.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr980
- DOI:
- 10.4238/vol10-1gmr980
Analysis of alterations to the transcriptome of Loquat (Eriobotrya japonica Lindl.) under low temperature stress via de novo sequencing
Authors: R.G. Gong, J. Lai, W. Yang, M.A. Liao, Z.H. Wang and G.L. Liang
Loquat (Eriobotrya japonica Lindl.), which originates from the cooler hill regions of southwestern China, is a typical subtropical evergreen tree. Loquat is one of the most important economic crops in China, but the available genomic information is very limited. Here, we present the first deep transcriptomic analysis of loquat. De novo assembly generated .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.6
- DOI:
- 10.4238/2015.August.14.6
Analysis of polymorphisms in the FUT1 and TAP1 genes and their influence on immune performance in Pudong White pigs
Authors: Y. Zhang, M. Wang, X.Q. Yu, C.R. Ye and J.G. Zhu
FUT1 and TAP1 have been identified as candidate genes that offer resistance against Escherichia coli F18 infection, with the AA genotype in FUT1 and the GG genotype in TAP1 conferring resistance. In order to confirm polymorphisms at FUT1 M307 and TAP1 G729, and evaluate their influence on immunity performance in Pudong White pigs, we performed polymerase .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.19
- DOI:
- 10.4238/2015.December.16.19
Investigation of ABCB1 gene polymorphism with colchicine response in Beh�§etâ��s disease
Authors: H. Saricaoglu, M. Yilmaz, M. Karkucak, H.Z.Y. Ozturk, T. Yakut, T. Gulten, E.B. Baskan, K. Aydogan and K. Dilek
Colchicine is commonly used in the treatment of Behçet’s disease. However, some patients are unresponsive to colchicine treatment. Adenosine triphosphate-binding cassette subfamily B member 1 (ABCB1) transports colchicine out of cells. We investigated a possible association of C3435T polymorphism of the ABCB1 (MDR.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr824
- DOI:
- 10.4238/vol10-1gmr824
Genetic variability of DNA repair mechanisms in chemotherapy treatment outcome of gastric cancer patients
Authors: G. Zhong, H.K. Li, T. Shan and N. Zhang
We investigate whether three common polymorphisms in ERCC1 and ERCC2 are predictor factors for the chemotherapy response, as well as the clinic outcome of patients with gastric cancer. Between May 2011 and May 2013, 263 patients with gastric cancer who were newly diagnosed by histopathology were enrolled in our study. Genotyping of the ERCC1 rs11615 and r.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.22
- DOI:
- 10.4238/2015.December.16.22
Amiloride, a urokinase-type plasminogen activator receptor (uTPA) inhibitor, reduces proteinurea in podocytes
Authors: L.B. Xu, N. Chi and W. Shi
This study examined the mechanism of action of amiloride, a urokinase-type plasminogen activator receptor inhibitor, in lowering proteinuria. Podocytes were resuscitated to allow for their proliferation and were observed for morphological changes. In the in vitro experiment, control, lipopolysaccharide, and lipopolysaccharide + amiloride groups were estab.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.15
- DOI:
- 10.4238/2015.August.14.15
An optimized preparation method to obtain high-quality RNA from dry sunflower seeds
Authors: X.B. Ma and J. Yang
In an attempt to isolate high-quality, intact total RNA from sunflower (Helianthus annuus) seeds for investigation of the molecular mechanisms of mutations, we tested various procedures, using kits, including RNAiso Plus, RNAiso Plus+RNAiso-mate for Plant Tissue, Trizol, and the Qi method, but no high-quality total RNA of high.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr979
- DOI:
- 10.4238/vol10-1gmr979
Single-primer PCR correction: a strategy for false-positive exclusion
Authors: J. Ma, P.W. Wang, D. Yao, Y.P. Wang, W. Yan and S.C. Guan
Polymerase chain reaction (PCR) technology plays an important role in molecular biology research, but false-positive and nonspecific PCR amplification have plagued many researchers. Currently, research on the optimization of the PCR system focuses on double-primer-based PCR products. This research has shown that PCR amplificat.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr988
- DOI:
- 10.4238/vol10-1gmr988
Associations between HIF-1�± polymorphisms C1772T and G1790A and susceptibility to chronic obstructive pulmonary disease
Authors: W.T. Wei, B. Li, M. Chen, H.R. Jia and H.X. Zhang
In the present study, we examined whether hypoxia-inducible factor-1α (HIF-1α) polymorphisms are associated with the susceptibility to chronic obstructive pulmonary disease (COPD). One hundred and twenty patients with COPD and 112 healthy controls were recruited from the Han population in Southern China. Polymerase chain reaction-restriction f.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.2
- DOI:
- 10.4238/2015.December.21.2