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Blockage of cisplatin-induced autophagy sensitizes cervical cancer cells to cisplatin
Authors: W.M. Lin and Z.G. Li
Development of chemoresistance is a major obstacle that leads to the recurrence and progression of cervical cancer (CC). Autophagy, meaning, “eating of self”, has shown paradoxical functions in tumors. In this study, we first investigated the process of autophagy induction by cisplatin in CC cells. Next, we investigated the role of autophagy i.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.14.18
- DOI:
- 10.4238/2015.December.14.18
Target replacement strategy for selection of DNA aptamers against the Fc region of mouse IgG
Authors: J. Ma, M.G. Wang, A.H. Mao, J.Y. Zeng, Y.Q. Liu, X.Q. Wang, J. Ma, Y.J. Tian, N. Ma, N. Yang, L. Wang and S.Q. Liao
Aptamers that recognize the IgG Fc region are of great interest because of their wide application as an immunology probing tool, for diagnostics, and as affinity agents for antibody purification. We developed a target replacement strategy as a modification of conventional Systematic Evolution of Ligands by EXponential enrichment (SELEX) in order to effici.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.25.11
- DOI:
- 10.4238/2013.April.25.11
Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense
Authors: N.M.A. Rahman, H. Fu, H. Qiao, S. Jin, H. Bai, W. Zhang, F.W. Jiang, G. Liang, S. Sun, Y. Gong, F.F. Jiang, Y. Xiong and Y. Wu
Feminization-1 homolog b (Fem1b) is one of the genes essential for male development and play central roles in sex determination of Caenorhabditis elegans. In this study, we cloned and characterized the full-length Fem1b cDNA from the freshwater prawn Macrobrachium nipponense (MnFem1b) in different tissues and at different deve.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027950
- DOI:
- 10.4238/gmr.15027950
Differential expression of lipid metabolism genes in the liver and adipose tissue of mice treated with evodiamine
Authors: D.F. Jiang, X.G. Zhang, H.L. Yang and C. Sun
Evodiamine is the main active alkali of Wu Zhuyu, a widely distributed Chinese herb. It plays an important role in the regulation of body fat deposition. In this study, we aimed to investigate the effect of evodiamine administration on the expression of genes involved in lipid metabolism in the liver and adipose tissue. Fasted mice were subcutaneously inj.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.January.4.13
- DOI:
- 10.4238/2013.January.4.13
Selection in sugarcane based on inbreeding depression
Authors: A.A.C. de Azeredo, L.L. Bhering, B.P. Brasileiro, C.D. Cruz and M.H.P. Barbosa
This study aimed to evaluate the gene action associated with yield-related traits, including mean stalk weight (MSW), tons of sugarcane per hectare (TCH), and fiber content (FIB) in sugarcane. Moreover, the viability of individual reciprocal recurrent selection (RRSI-S1) was verified, and the effect of inbreeding depression on.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027965
- DOI:
- 10.4238/gmr.15027965
CYP2C19 polymorphisms in acute coronary syndrome patients undergoing clopidogrel therapy in Zhengzhou population
Authors: Y.M. Guo, Z.C. Zhao, L. Zhang, H.Z. Li, Z. Li and H.L. Sun
The goal of this study was to explore the polymorphisms of CYP2C19 (CYP2C19*2, CYP2C19*3) in patients with acute coronary syndrome (ACS) undergoing percutaneous coronary intervention (PCI) on clopidogrel therapy in Zhengzhou city for guidance on clinical medication and reduction in the incidence of thromboembolic events. Two h.. Read More»
- Genet. Mol. Res. 15(2):
Application of retrograde dissection method for isolation of bone marrow cells from rat femurs and tibiae
Authors: C.M. Li, B.M. Fu, L.C. Zhang, B. Tang, L. Zhu, Y. Zhao and J. Zhang
Currently, there is no practical and efficient method for the isolation of bone marrow cells (BMCs) from rat femurs and tibiae. Here, we attempted to develop a rapid, simple, effective, and non-contaminating method for the isolation of BMCs from rat femurs and tibiae. Rat femurs and tibiae were dissected from the ankle to the .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028178
- DOI:
- 10.4238/gmr.15028178
Relationship between TBX20 gene polymorphism and congenital heart disease
Authors: X.F. Yang, Y.F. Zhang, C.F. Zhao, M.M. Liu, J.P. Si, Y.F. Fang,W.W. Xing and F.L. Wang
Congenital heart disease in children is a type of birth defect. Previous studies have suggested that the transcription factor, TBX20, is involved in the occurrence and development of congenital heart disease in children; however, the specific regulatory mechanisms are yet to be evaluated. Hence, this study aimed to evaluate th.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027374
- DOI:
- 10.4238/gmr.15027374
Gene expression and enzyme activity of lipoprotein lipase correlate with intramuscular fat content in Guangxi san-huang and Arbor Acres chickens
Authors: Y.N. Huang, J. Wang, B.J. Chen, Q.Y. Jiang, Y.F. Guo, G.Q. Lan and H.S. Jiang
Lipoprotein lipase (LPL) is a key enzyme in lipid metabolism. This study investigated LPL gene expression, LPL enzyme activity, and the correlation of each with intramuscular fat (IMF) in Chinese Guangxi san-huang (GXSH) and Arbor Acres (AA) chickens. The results showed that age and breed had significant effects on LPL express.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027414
- DOI:
- 10.4238/gmr.15027414
Translation of foot-and-mouth disease virus RNA: factors influencing alternative AUG selection
Authors: J.L. Liu, Y.H. Ma, J.Y. Xu, Y.X. Chen, S.E. Chen and Z.R. Ma
The mechanism of alternative AUG usage in foot-and-mouth disease virus is not completely understood. Using simple computational approaches, we evaluated the contributions of overall codon bias, quantitative codon bias, and %GC of the region between the two alternative AUGs, Region-La, as well as the nucleotide bias of the sequence context flanking each AU.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.14.7
- DOI:
- 10.4238/2015.December.14.7